Unveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activities
Abstract The quest for eco-friendly and biocompatible nanoparticles (NPs) is an urgent issue in the agenda of the scientific community and applied technology, which compressing synthesis routes. For the first time, a simple route for the biosynthesis of functionalized CuFe-hybrid nanocomposites (FCF...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-87363-z |
| _version_ | 1828958841757237248 |
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| author | Marwa Eltarahony Marwa Abu-Serie Hesham Hamad Sahar Zaki Desouky Abd-El-Haleem |
| author_facet | Marwa Eltarahony Marwa Abu-Serie Hesham Hamad Sahar Zaki Desouky Abd-El-Haleem |
| author_sort | Marwa Eltarahony |
| collection | DOAJ |
| description | Abstract The quest for eco-friendly and biocompatible nanoparticles (NPs) is an urgent issue in the agenda of the scientific community and applied technology, which compressing synthesis routes. For the first time, a simple route for the biosynthesis of functionalized CuFe-hybrid nanocomposites (FCFNCs) was achieved using Streptomyces cyaneofuscatus through a simultaneous bioreduction strategy of Cu and Fe salts. The suitability of FCFNCs was evaluated medically and environmentally as an anticancer agent, antimicrobial agent and dye bio-sorbent. The physicochemical characteristics of FCFNCs using XRD, EDX, elemental mapping, FTIR, UV–Vis., TEM and ζ-potential confirmed the formation of spheres agglomerated into chains (37 ± 2.2 nm), self-functionalized nanocomposite by proteinaceous moieties with considerable stability (− 26.2 mV). As an anticancer agent, FCFNCs displayed the highest apoptotic impact (> 77.7%) on Caco-2, HepG-2, MCF-7 and PC-3 cancer cells at IC50 ≤ 17.21 μg/mL with the maximum up regulation of p53 and caspase 3 expression and the lowest Ki-67 level, relative to both functionalized CuNPs (FCNPs) and FeNPs (FFNPs). Meanwhile, it maintained the viability of normal human cells by EC100 up to 1999.7 μg/mL. Regarding the antimicrobial activity, FCFNCs offered > 70% growth reduction among wide spectrum prokaryotic and eukaryotic pathogens. Additionally, the synergistic feature of FCFNCs disintegrated the pre-established biofilm and algal growth in a dose-dependent manner. However, as a bio-sorbent, FCFNCs decolorized > 68% of malachite green and congo red dyes (200 mg/L), reflecting considerable remediation efficiency, confirmed by FTIR of FCFNCs- adsorbed dyes and microtoxicity/cytotoxicity of solutions after remediation. This study offers new insights into promising CuFe-hybrid nanocomposites for recruitment in several applications. |
| first_indexed | 2024-12-14T08:56:33Z |
| format | Article |
| id | doaj.art-0f593892e22a43b1a35270acafcec14a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T08:56:33Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0f593892e22a43b1a35270acafcec14a2022-12-21T23:08:55ZengNature PortfolioScientific Reports2045-23222021-04-0111112210.1038/s41598-021-87363-zUnveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activitiesMarwa Eltarahony0Marwa Abu-Serie1Hesham Hamad2Sahar Zaki3Desouky Abd-El-Haleem4Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City)Medical Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, (GEBRI), City of Scientific Research and Technological Applications (SRTA-City)Fabrication Technology Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City)Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City)Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City)Abstract The quest for eco-friendly and biocompatible nanoparticles (NPs) is an urgent issue in the agenda of the scientific community and applied technology, which compressing synthesis routes. For the first time, a simple route for the biosynthesis of functionalized CuFe-hybrid nanocomposites (FCFNCs) was achieved using Streptomyces cyaneofuscatus through a simultaneous bioreduction strategy of Cu and Fe salts. The suitability of FCFNCs was evaluated medically and environmentally as an anticancer agent, antimicrobial agent and dye bio-sorbent. The physicochemical characteristics of FCFNCs using XRD, EDX, elemental mapping, FTIR, UV–Vis., TEM and ζ-potential confirmed the formation of spheres agglomerated into chains (37 ± 2.2 nm), self-functionalized nanocomposite by proteinaceous moieties with considerable stability (− 26.2 mV). As an anticancer agent, FCFNCs displayed the highest apoptotic impact (> 77.7%) on Caco-2, HepG-2, MCF-7 and PC-3 cancer cells at IC50 ≤ 17.21 μg/mL with the maximum up regulation of p53 and caspase 3 expression and the lowest Ki-67 level, relative to both functionalized CuNPs (FCNPs) and FeNPs (FFNPs). Meanwhile, it maintained the viability of normal human cells by EC100 up to 1999.7 μg/mL. Regarding the antimicrobial activity, FCFNCs offered > 70% growth reduction among wide spectrum prokaryotic and eukaryotic pathogens. Additionally, the synergistic feature of FCFNCs disintegrated the pre-established biofilm and algal growth in a dose-dependent manner. However, as a bio-sorbent, FCFNCs decolorized > 68% of malachite green and congo red dyes (200 mg/L), reflecting considerable remediation efficiency, confirmed by FTIR of FCFNCs- adsorbed dyes and microtoxicity/cytotoxicity of solutions after remediation. This study offers new insights into promising CuFe-hybrid nanocomposites for recruitment in several applications.https://doi.org/10.1038/s41598-021-87363-z |
| spellingShingle | Marwa Eltarahony Marwa Abu-Serie Hesham Hamad Sahar Zaki Desouky Abd-El-Haleem Unveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activities Scientific Reports |
| title | Unveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activities |
| title_full | Unveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activities |
| title_fullStr | Unveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activities |
| title_full_unstemmed | Unveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activities |
| title_short | Unveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activities |
| title_sort | unveiling the role of novel biogenic functionalized cufe hybrid nanocomposites in boosting anticancer antimicrobial and biosorption activities |
| url | https://doi.org/10.1038/s41598-021-87363-z |
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