Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of <i>Macrobrachium nipponense</i>
A TMT-based (Tandem Mass Tag) liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs) and KEGG pathways in hepatopancreas of 5 ovary stages. In total, 17,999 peptides were detected, among which 3395 proteins were...
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MDPI AG
2023-03-01
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| author | Sufei Jiang Hui Qiao Hongtuo Fu Zemao Gu |
| author_facet | Sufei Jiang Hui Qiao Hongtuo Fu Zemao Gu |
| author_sort | Sufei Jiang |
| collection | DOAJ |
| description | A TMT-based (Tandem Mass Tag) liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs) and KEGG pathways in hepatopancreas of 5 ovary stages. In total, 17,999 peptides were detected, among which 3395 proteins were identified. Further analysis revealed 26, 24, 37, and 308 DEPs in HE-I versus HE-II, HE-II versus HE-Ⅲ, HE-Ⅲ versus HE-Ⅳ, and HE-Ⅳ versus HE-Ⅴ, respectively (HE-I, HE-II, HE-III, HE-IV, and HE-V means hepatopancreas sampled from ovary stage I to V.). Gene ontology (GO) analysis indicated that DEPs were significantly enriched in “catalytic activity”, “metabolic process”, and “cell” of 4 comparison groups in turn. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results showed that in hepatopancreas, as the ovaries developed to maturation, carbohydrate metabolism, lipid metabolism, amino acid metabolism, and lysosome played important roles in turn. The mRNA expression of 15 selected DEPs were consistent with proteome results by qPCR analysis. Further mRNA expression investigation results suggested 4 proteins (fatty acid-binding protein, NPC intracellular cholesterol transporter 1, Serine hydroxymethyltransferase, and Crustapin) were involved in ovary maturation. These results enhance the understanding of the regulatory role of hepatopancreas in <i>M. nipponense</i> ovary maturation and provide new insights for understanding the crustacean regulation mechanisms. |
| first_indexed | 2024-03-11T07:02:11Z |
| format | Article |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T07:02:11Z |
| publishDate | 2023-03-01 |
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| series | Animals |
| spelling | doaj.art-0f5a34cd78d341ada90842b597b404642023-11-17T09:10:32ZengMDPI AGAnimals2076-26152023-03-0113697710.3390/ani13060977Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of <i>Macrobrachium nipponense</i>Sufei Jiang0Hui Qiao1Hongtuo Fu2Zemao Gu3College of Fisheries, Shuangshui Shuanglü Institute, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaCollege of Fisheries, Shuangshui Shuanglü Institute, Huazhong Agricultural University, Wuhan 430070, ChinaA TMT-based (Tandem Mass Tag) liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics approach was employed to explore differentially expressed proteins (DEPs) and KEGG pathways in hepatopancreas of 5 ovary stages. In total, 17,999 peptides were detected, among which 3395 proteins were identified. Further analysis revealed 26, 24, 37, and 308 DEPs in HE-I versus HE-II, HE-II versus HE-Ⅲ, HE-Ⅲ versus HE-Ⅳ, and HE-Ⅳ versus HE-Ⅴ, respectively (HE-I, HE-II, HE-III, HE-IV, and HE-V means hepatopancreas sampled from ovary stage I to V.). Gene ontology (GO) analysis indicated that DEPs were significantly enriched in “catalytic activity”, “metabolic process”, and “cell” of 4 comparison groups in turn. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results showed that in hepatopancreas, as the ovaries developed to maturation, carbohydrate metabolism, lipid metabolism, amino acid metabolism, and lysosome played important roles in turn. The mRNA expression of 15 selected DEPs were consistent with proteome results by qPCR analysis. Further mRNA expression investigation results suggested 4 proteins (fatty acid-binding protein, NPC intracellular cholesterol transporter 1, Serine hydroxymethyltransferase, and Crustapin) were involved in ovary maturation. These results enhance the understanding of the regulatory role of hepatopancreas in <i>M. nipponense</i> ovary maturation and provide new insights for understanding the crustacean regulation mechanisms.https://www.mdpi.com/2076-2615/13/6/977<i>Macrobrachium nipponense</i>comparative proteomicshepatopancreasovary maturation |
| spellingShingle | Sufei Jiang Hui Qiao Hongtuo Fu Zemao Gu Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of <i>Macrobrachium nipponense</i> Animals <i>Macrobrachium nipponense</i> comparative proteomics hepatopancreas ovary maturation |
| title | Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of <i>Macrobrachium nipponense</i> |
| title_full | Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of <i>Macrobrachium nipponense</i> |
| title_fullStr | Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of <i>Macrobrachium nipponense</i> |
| title_full_unstemmed | Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of <i>Macrobrachium nipponense</i> |
| title_short | Hepatopancreas Proteomic Analysis Reveals Key Proteins and Pathways in Regulatory of Ovary Maturation of <i>Macrobrachium nipponense</i> |
| title_sort | hepatopancreas proteomic analysis reveals key proteins and pathways in regulatory of ovary maturation of i macrobrachium nipponense i |
| topic | <i>Macrobrachium nipponense</i> comparative proteomics hepatopancreas ovary maturation |
| url | https://www.mdpi.com/2076-2615/13/6/977 |
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