The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress
The effect of 24-epibrassinolide and 28-homobrassinolide on seed germination and seedling growth of radish (Raphanus sativus L.) was studied under cadmium toxicity. The impact of brassinosteroids (BRs) on free proline levels and the activity of antioxidant enzymes catalase (CAT; EC 1.11.1.6), peroxi...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2007-11-01
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| Series: | Plant, Soil and Environment |
| Subjects: | |
| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-200711-0001_the-effect-of-brassinosteroids-on-radish-raphanus-sativus-l-seedlings-growing-under-cadmium-stress.php |
| _version_ | 1828007526045581312 |
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| author | S. Anuradha S.S.R. Rao |
| author_facet | S. Anuradha S.S.R. Rao |
| author_sort | S. Anuradha |
| collection | DOAJ |
| description | The effect of 24-epibrassinolide and 28-homobrassinolide on seed germination and seedling growth of radish (Raphanus sativus L.) was studied under cadmium toxicity. The impact of brassinosteroids (BRs) on free proline levels and the activity of antioxidant enzymes catalase (CAT; EC 1.11.1.6), peroxidase (POD; EC 1.11.1.7), superoxide dismutase (SOD; EC 1.15.1.1), ascorbic peroxidase (APOX; EC 1.11.1.11) and guaiacol peroxidase (GPX; EC 1.11.1.7) in radish seedlings under Cd toxicity was evaluated. The effect of BRs on the activity of ascorbic acid oxidase (AAO; EC 1.10.3.3) and lipid peroxidation in radish seedlings challenged with Cd stress was also investigated. BRs supplementation alleviated the toxic effect of the heavy metal and increased the percentage of seed germination and seedling growth. Out of the two substances, HBL was found to be more effective than EBL in stress alleviation. HBL (3µM) alleviated the toxic effect of the heavy metal and increased the percentage of seed germination by 57% over Cd and 20% over unstressed control. Similarly supplementation of HBL (3µM) caused an increase of 156%, 78% and 91% in length, fresh weight and dry weight of seedling, respectively, over Cd treatment alone. The amelioration of seedling growth by BRs under metal toxicity was associated with enhanced levels of free proline. The activities of antioxidant enzymes CAT, SOD, APOX and GPX were increased in the seedlings from treatments with Cd along with BRs. Brassinosteroid treatment reduced the activity of POD and AAO in heavy metal stressed seedlings. Lipid peroxidation induced by Cd was found reduced with the supplementation of BRs. The results obtained in the study clearly indicated the ameliorative influence of brassinosteroids on the inhibitory effect of Cd toxicity. |
| first_indexed | 2024-04-10T08:11:35Z |
| format | Article |
| id | doaj.art-0f5b44877dda4593b217e5503c474f26 |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:11:35Z |
| publishDate | 2007-11-01 |
| publisher | Czech Academy of Agricultural Sciences |
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| series | Plant, Soil and Environment |
| spelling | doaj.art-0f5b44877dda4593b217e5503c474f262023-02-23T03:45:19ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682007-11-01531146547210.17221/2307-PSEpse-200711-0001The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stressS. Anuradha0S.S.R. Rao1Department of Botany, Osmania University, Hyderabad, IndiaDepartment of Botany, Osmania University, Hyderabad, IndiaThe effect of 24-epibrassinolide and 28-homobrassinolide on seed germination and seedling growth of radish (Raphanus sativus L.) was studied under cadmium toxicity. The impact of brassinosteroids (BRs) on free proline levels and the activity of antioxidant enzymes catalase (CAT; EC 1.11.1.6), peroxidase (POD; EC 1.11.1.7), superoxide dismutase (SOD; EC 1.15.1.1), ascorbic peroxidase (APOX; EC 1.11.1.11) and guaiacol peroxidase (GPX; EC 1.11.1.7) in radish seedlings under Cd toxicity was evaluated. The effect of BRs on the activity of ascorbic acid oxidase (AAO; EC 1.10.3.3) and lipid peroxidation in radish seedlings challenged with Cd stress was also investigated. BRs supplementation alleviated the toxic effect of the heavy metal and increased the percentage of seed germination and seedling growth. Out of the two substances, HBL was found to be more effective than EBL in stress alleviation. HBL (3µM) alleviated the toxic effect of the heavy metal and increased the percentage of seed germination by 57% over Cd and 20% over unstressed control. Similarly supplementation of HBL (3µM) caused an increase of 156%, 78% and 91% in length, fresh weight and dry weight of seedling, respectively, over Cd treatment alone. The amelioration of seedling growth by BRs under metal toxicity was associated with enhanced levels of free proline. The activities of antioxidant enzymes CAT, SOD, APOX and GPX were increased in the seedlings from treatments with Cd along with BRs. Brassinosteroid treatment reduced the activity of POD and AAO in heavy metal stressed seedlings. Lipid peroxidation induced by Cd was found reduced with the supplementation of BRs. The results obtained in the study clearly indicated the ameliorative influence of brassinosteroids on the inhibitory effect of Cd toxicity.https://pse.agriculturejournals.cz/artkey/pse-200711-0001_the-effect-of-brassinosteroids-on-radish-raphanus-sativus-l-seedlings-growing-under-cadmium-stress.phpbrassinosteroidsradishcadmiumseedling growthcatalaseperoxidaseprolinesuperoxide dismutase |
| spellingShingle | S. Anuradha S.S.R. Rao The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress Plant, Soil and Environment brassinosteroids radish cadmium seedling growth catalase peroxidase proline superoxide dismutase |
| title | The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress |
| title_full | The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress |
| title_fullStr | The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress |
| title_full_unstemmed | The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress |
| title_short | The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress |
| title_sort | effect of brassinosteroids on radish raphanus sativus l seedlings growing under cadmium stress |
| topic | brassinosteroids radish cadmium seedling growth catalase peroxidase proline superoxide dismutase |
| url | https://pse.agriculturejournals.cz/artkey/pse-200711-0001_the-effect-of-brassinosteroids-on-radish-raphanus-sativus-l-seedlings-growing-under-cadmium-stress.php |
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