Synovial mast cells from knee and hip osteoarthritis: histological study and clinical correlations
Abstract Purpose The aim of this study was to investigate the presence of synovial mast cells (MCs) in hip and knee tissue from osteoarthritis (OA) patients and to correlate them with clinical and radiological data. Methods Synovial tissue was obtained during arthroplasty from 60 patients, 30 with k...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Experimental Orthopaedics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40634-022-00446-2 |
| _version_ | 1827356322075508736 |
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| author | L. Farinelli A. Aquili M. Mattioli‐Belmonte S. Manzotti F. D’Angelo C. Ciccullo A. Gigante |
| author_facet | L. Farinelli A. Aquili M. Mattioli‐Belmonte S. Manzotti F. D’Angelo C. Ciccullo A. Gigante |
| author_sort | L. Farinelli |
| collection | DOAJ |
| description | Abstract Purpose The aim of this study was to investigate the presence of synovial mast cells (MCs) in hip and knee tissue from osteoarthritis (OA) patients and to correlate them with clinical and radiological data. Methods Synovial tissue was obtained during arthroplasty from 60 patients, 30 with knee OA and 30 with hip OA. Control synovial tissue was obtained from 30 patients without OA, 15 undergoing above‐knee amputation and 15 receiving a hip replacement for fracture. Before surgery, the radiographic findings were graded according to the Kellgren‐Lawrence system and clinical data including pain (VAS) and functional information (KOOS and HOOS) was collected. The tissue was stained with hematoxylin–eosin and toluidine blue for histochemistry and incubated with CD117 and CD31 antibodies for immunohistochemistry. MC and vessel number and synovitis score were determined in all samples. Results Mean MC number, synovitis score and vessel number were significantly higher in the OA samples (p < 0.05) than in control tissue. MC number correlated with the synovitis score and disease severity in both patient groups. Conclusions The prevalence of MCs in synovium from OA patients and their association with synovial inflammation and pain suggest a role for them in OA pathophysiology. |
| first_indexed | 2024-03-08T04:59:04Z |
| format | Article |
| id | doaj.art-0f5e83920ab24dbc9f65ae9a3cd47b80 |
| institution | Directory Open Access Journal |
| issn | 2197-1153 |
| language | English |
| last_indexed | 2024-03-08T04:59:04Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Experimental Orthopaedics |
| spelling | doaj.art-0f5e83920ab24dbc9f65ae9a3cd47b802024-02-07T14:50:36ZengWileyJournal of Experimental Orthopaedics2197-11532022-01-0191n/an/a10.1186/s40634-022-00446-2Synovial mast cells from knee and hip osteoarthritis: histological study and clinical correlationsL. Farinelli0A. Aquili1M. Mattioli‐Belmonte2S. Manzotti3F. D’Angelo4C. Ciccullo5A. Gigante6Clinical OrthopaedicsDepartment of Clinical and Molecular Science DISCLIMOUniversità Politecnica Delle MarcheAnconaItalyClinical OrthopaedicsDepartment of Clinical and Molecular Science DISCLIMOUniversità Politecnica Delle MarcheAnconaItalyDepartment of Clinical and Molecular Science DISCLIMOUniversità Politecnica Delle MarcheAnconaItalyClinical OrthopaedicsDepartment of Clinical and Molecular Science DISCLIMOUniversità Politecnica Delle MarcheAnconaItalyClinical OrthopaedicsDepartment of Clinical and Molecular Science DISCLIMOUniversità Politecnica Delle MarcheAnconaItalyClinical OrthopaedicsDepartment of Clinical and Molecular Science DISCLIMOUniversità Politecnica Delle MarcheAnconaItalyClinical OrthopaedicsDepartment of Clinical and Molecular Science DISCLIMOUniversità Politecnica Delle MarcheAnconaItalyAbstract Purpose The aim of this study was to investigate the presence of synovial mast cells (MCs) in hip and knee tissue from osteoarthritis (OA) patients and to correlate them with clinical and radiological data. Methods Synovial tissue was obtained during arthroplasty from 60 patients, 30 with knee OA and 30 with hip OA. Control synovial tissue was obtained from 30 patients without OA, 15 undergoing above‐knee amputation and 15 receiving a hip replacement for fracture. Before surgery, the radiographic findings were graded according to the Kellgren‐Lawrence system and clinical data including pain (VAS) and functional information (KOOS and HOOS) was collected. The tissue was stained with hematoxylin–eosin and toluidine blue for histochemistry and incubated with CD117 and CD31 antibodies for immunohistochemistry. MC and vessel number and synovitis score were determined in all samples. Results Mean MC number, synovitis score and vessel number were significantly higher in the OA samples (p < 0.05) than in control tissue. MC number correlated with the synovitis score and disease severity in both patient groups. Conclusions The prevalence of MCs in synovium from OA patients and their association with synovial inflammation and pain suggest a role for them in OA pathophysiology.https://doi.org/10.1186/s40634-022-00446-2OsteoarthritisSynovial mast cellsSynoviumInflammationLevel of evidenceBasic science study |
| spellingShingle | L. Farinelli A. Aquili M. Mattioli‐Belmonte S. Manzotti F. D’Angelo C. Ciccullo A. Gigante Synovial mast cells from knee and hip osteoarthritis: histological study and clinical correlations Journal of Experimental Orthopaedics Osteoarthritis Synovial mast cells Synovium Inflammation Level of evidence Basic science study |
| title | Synovial mast cells from knee and hip osteoarthritis: histological study and clinical correlations |
| title_full | Synovial mast cells from knee and hip osteoarthritis: histological study and clinical correlations |
| title_fullStr | Synovial mast cells from knee and hip osteoarthritis: histological study and clinical correlations |
| title_full_unstemmed | Synovial mast cells from knee and hip osteoarthritis: histological study and clinical correlations |
| title_short | Synovial mast cells from knee and hip osteoarthritis: histological study and clinical correlations |
| title_sort | synovial mast cells from knee and hip osteoarthritis histological study and clinical correlations |
| topic | Osteoarthritis Synovial mast cells Synovium Inflammation Level of evidence Basic science study |
| url | https://doi.org/10.1186/s40634-022-00446-2 |
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