OCT4 increases BIRC5 and CCND1 expression and promotes cancer progression in hepatocellular carcinoma
<p>Abstract</p> <p>Background</p> <p>OCT4 and BIRC5 are preferentially expressed in human cancer cells and mediate cancer cell survival and tumor maintenance. However, the molecular mechanism that regulates OCT4 and BIRC5 expression is not well characterized.</p>...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2013-02-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2407/13/82 |
| _version_ | 1818146673908514816 |
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| author | Cao Lu Li Chunguang Shen Shuwen Yan Yan Ji Weidan Wang Jinghan Qian Haihua Jiang Xiaoqing Li Zhigang Wu Mengchao Zhang Ying Su Changqing |
| author_facet | Cao Lu Li Chunguang Shen Shuwen Yan Yan Ji Weidan Wang Jinghan Qian Haihua Jiang Xiaoqing Li Zhigang Wu Mengchao Zhang Ying Su Changqing |
| author_sort | Cao Lu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>OCT4 and BIRC5 are preferentially expressed in human cancer cells and mediate cancer cell survival and tumor maintenance. However, the molecular mechanism that regulates OCT4 and BIRC5 expression is not well characterized.</p> <p>Methods</p> <p>By manipulating OCT4 and BIRC5 expression in hepatocellular carcinoma (HCC) cell lines, the regulatory mechanism of OCT4 on BIRC5 and CCND1 were investigated.</p> <p>Results</p> <p>Increasing or decreasing OCT4 expression could enhance or suppress BIRC5 expression, respectively, by regulating the activity of BIRC5 promoter. Because there is no binding site for OCT4 within BIRC5 promoter, the effect of OCT4 on BIRC5 promoter is indirect. An octamer motif for OCT4 in the CCND1 promoter has directly and partly participated in the regulation of CCND1 promoter activity, suggesting that OCT4 also could upregulated the expression of CCND1. Co-suppression of OCT4 and BIRC5 induced cancer cell apoptosis and cell cycle arrest, thereby efficiently inhibiting the proliferative activity of cancer cells and suppressing the growth of HCC xenogrfts in nude mice.</p> <p>Conclusion</p> <p>OCT4 can upregulate BIRC5 and CCND1 expression by increasing their promoter activity. These factors collusively promotes HCC cell proliferation, and co-suppression of OCT4 and BIRC5 is potentially beneficial for HCC treatment.</p> |
| first_indexed | 2024-12-11T12:23:06Z |
| format | Article |
| id | doaj.art-0f5fa028630b4202af087df6217d6d41 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-11T12:23:06Z |
| publishDate | 2013-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-0f5fa028630b4202af087df6217d6d412022-12-22T01:07:28ZengBMCBMC Cancer1471-24072013-02-011318210.1186/1471-2407-13-82OCT4 increases BIRC5 and CCND1 expression and promotes cancer progression in hepatocellular carcinomaCao LuLi ChunguangShen ShuwenYan YanJi WeidanWang JinghanQian HaihuaJiang XiaoqingLi ZhigangWu MengchaoZhang YingSu Changqing<p>Abstract</p> <p>Background</p> <p>OCT4 and BIRC5 are preferentially expressed in human cancer cells and mediate cancer cell survival and tumor maintenance. However, the molecular mechanism that regulates OCT4 and BIRC5 expression is not well characterized.</p> <p>Methods</p> <p>By manipulating OCT4 and BIRC5 expression in hepatocellular carcinoma (HCC) cell lines, the regulatory mechanism of OCT4 on BIRC5 and CCND1 were investigated.</p> <p>Results</p> <p>Increasing or decreasing OCT4 expression could enhance or suppress BIRC5 expression, respectively, by regulating the activity of BIRC5 promoter. Because there is no binding site for OCT4 within BIRC5 promoter, the effect of OCT4 on BIRC5 promoter is indirect. An octamer motif for OCT4 in the CCND1 promoter has directly and partly participated in the regulation of CCND1 promoter activity, suggesting that OCT4 also could upregulated the expression of CCND1. Co-suppression of OCT4 and BIRC5 induced cancer cell apoptosis and cell cycle arrest, thereby efficiently inhibiting the proliferative activity of cancer cells and suppressing the growth of HCC xenogrfts in nude mice.</p> <p>Conclusion</p> <p>OCT4 can upregulate BIRC5 and CCND1 expression by increasing their promoter activity. These factors collusively promotes HCC cell proliferation, and co-suppression of OCT4 and BIRC5 is potentially beneficial for HCC treatment.</p>http://www.biomedcentral.com/1471-2407/13/82Transcription factorCell cycleCell apoptosisCancer biotherapyHepatocellular carcinoma |
| spellingShingle | Cao Lu Li Chunguang Shen Shuwen Yan Yan Ji Weidan Wang Jinghan Qian Haihua Jiang Xiaoqing Li Zhigang Wu Mengchao Zhang Ying Su Changqing OCT4 increases BIRC5 and CCND1 expression and promotes cancer progression in hepatocellular carcinoma BMC Cancer Transcription factor Cell cycle Cell apoptosis Cancer biotherapy Hepatocellular carcinoma |
| title | OCT4 increases BIRC5 and CCND1 expression and promotes cancer progression in hepatocellular carcinoma |
| title_full | OCT4 increases BIRC5 and CCND1 expression and promotes cancer progression in hepatocellular carcinoma |
| title_fullStr | OCT4 increases BIRC5 and CCND1 expression and promotes cancer progression in hepatocellular carcinoma |
| title_full_unstemmed | OCT4 increases BIRC5 and CCND1 expression and promotes cancer progression in hepatocellular carcinoma |
| title_short | OCT4 increases BIRC5 and CCND1 expression and promotes cancer progression in hepatocellular carcinoma |
| title_sort | oct4 increases birc5 and ccnd1 expression and promotes cancer progression in hepatocellular carcinoma |
| topic | Transcription factor Cell cycle Cell apoptosis Cancer biotherapy Hepatocellular carcinoma |
| url | http://www.biomedcentral.com/1471-2407/13/82 |
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