Investigation of the Initial Host Response of Naïve Atlantic Salmon (<i>Salmo salar</i>) Inoculated with <i>Paramoeba perurans</i>
Amoebic Gill Disease (AGD), caused by the ectoparasite <i>Paramoeba perurans</i> is characterised by hyperplasia of the gill epithelium and lamellar fusion. In this study, the initial host response of naïve Atlantic salmon (<i>Salmo salar</i>) inoculated with <i>P. peru...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/4/746 |
| _version_ | 1797539008114327552 |
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| author | Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy |
| author_facet | Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy |
| author_sort | Michelle McCormack |
| collection | DOAJ |
| description | Amoebic Gill Disease (AGD), caused by the ectoparasite <i>Paramoeba perurans</i> is characterised by hyperplasia of the gill epithelium and lamellar fusion. In this study, the initial host response of naïve Atlantic salmon (<i>Salmo salar</i>) inoculated with <i>P. perurans</i> was investigated. Using gel-free proteomic techniques and mass spectrometry gill and serum samples were analysed at 7 timepoints (2, 3, 4, 7, 9, 11 and 14 days) post-inoculation with <i>P. perurans</i>. Differential expression of immune related proteins was assessed by comparison of protein expression from each time point against naïve controls. Few host immune molecules associated with innate immunity showed increased expression in response to gill colonisation by amoebae. Furthermore, many proteins with roles in immune signalling, phagocytosis and T-cell proliferation were found to be inhibited upon disease progression. Initially, various immune factors demonstrated the anticipated increase in expression in response to infection in the serum while some immune inhibition became apparent at the later stages of disease progression. Taken together, the pro-immune trend observed in serum, the lack of a robust early immune response in the gill and the diversity of those proteins in the gill whose altered expression negatively impact the immune response, support the concept of a pathogen-derived suppression of the host response. |
| first_indexed | 2024-03-10T12:39:17Z |
| format | Article |
| id | doaj.art-0f6188812d2446eba9af34f11e8e1792 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T12:39:17Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0f6188812d2446eba9af34f11e8e17922023-11-21T13:58:30ZengMDPI AGMicroorganisms2076-26072021-04-019474610.3390/microorganisms9040746Investigation of the Initial Host Response of Naïve Atlantic Salmon (<i>Salmo salar</i>) Inoculated with <i>Paramoeba perurans</i>Michelle McCormack0Eugene Dillon1Ian O’Connor2Eugene MacCarthy3Marine and Freshwater Research Centre, Galway Mayo Institute of Technology, Dublin Road, H91 TRNW Galway, IrelandConway Institute of Biomolecular & Biomedical Research, University College Dublin, D04 V1W8 Dublin, IrelandMarine and Freshwater Research Centre, Galway Mayo Institute of Technology, Dublin Road, H91 TRNW Galway, IrelandMarine and Freshwater Research Centre, Galway Mayo Institute of Technology, Dublin Road, H91 TRNW Galway, IrelandAmoebic Gill Disease (AGD), caused by the ectoparasite <i>Paramoeba perurans</i> is characterised by hyperplasia of the gill epithelium and lamellar fusion. In this study, the initial host response of naïve Atlantic salmon (<i>Salmo salar</i>) inoculated with <i>P. perurans</i> was investigated. Using gel-free proteomic techniques and mass spectrometry gill and serum samples were analysed at 7 timepoints (2, 3, 4, 7, 9, 11 and 14 days) post-inoculation with <i>P. perurans</i>. Differential expression of immune related proteins was assessed by comparison of protein expression from each time point against naïve controls. Few host immune molecules associated with innate immunity showed increased expression in response to gill colonisation by amoebae. Furthermore, many proteins with roles in immune signalling, phagocytosis and T-cell proliferation were found to be inhibited upon disease progression. Initially, various immune factors demonstrated the anticipated increase in expression in response to infection in the serum while some immune inhibition became apparent at the later stages of disease progression. Taken together, the pro-immune trend observed in serum, the lack of a robust early immune response in the gill and the diversity of those proteins in the gill whose altered expression negatively impact the immune response, support the concept of a pathogen-derived suppression of the host response.https://www.mdpi.com/2076-2607/9/4/746amoebic gill diseaseAtlantic salmongel-free proteomicsgill proteomeserum proteome |
| spellingShingle | Michelle McCormack Eugene Dillon Ian O’Connor Eugene MacCarthy Investigation of the Initial Host Response of Naïve Atlantic Salmon (<i>Salmo salar</i>) Inoculated with <i>Paramoeba perurans</i> Microorganisms amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome |
| title | Investigation of the Initial Host Response of Naïve Atlantic Salmon (<i>Salmo salar</i>) Inoculated with <i>Paramoeba perurans</i> |
| title_full | Investigation of the Initial Host Response of Naïve Atlantic Salmon (<i>Salmo salar</i>) Inoculated with <i>Paramoeba perurans</i> |
| title_fullStr | Investigation of the Initial Host Response of Naïve Atlantic Salmon (<i>Salmo salar</i>) Inoculated with <i>Paramoeba perurans</i> |
| title_full_unstemmed | Investigation of the Initial Host Response of Naïve Atlantic Salmon (<i>Salmo salar</i>) Inoculated with <i>Paramoeba perurans</i> |
| title_short | Investigation of the Initial Host Response of Naïve Atlantic Salmon (<i>Salmo salar</i>) Inoculated with <i>Paramoeba perurans</i> |
| title_sort | investigation of the initial host response of naive atlantic salmon i salmo salar i inoculated with i paramoeba perurans i |
| topic | amoebic gill disease Atlantic salmon gel-free proteomics gill proteome serum proteome |
| url | https://www.mdpi.com/2076-2607/9/4/746 |
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