Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity
Endothelial progenitor cells and circulating endothelial cells have been proposed as useful markers of severity and disease progression in certain vascular diseases, including pulmonary arterial hypertension. Our study focused on evaluating the levels of circulating endothelial progenitor cells and...
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Frontiers Media S.A.
2023-07-01
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Series: | Frontiers in Pediatrics |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fped.2023.1200395/full |
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author | Juan Calderón-Colmenero Felipe Massó Héctor González-Pacheco Julio Sandoval Carlos Guerrero Jorge Cervantes-Salazar José A. García-Montes Araceli Paéz Gabriela I. Pereira-López Carlos Zabal-Cerdeira Juan Pablo Sandoval |
author_facet | Juan Calderón-Colmenero Felipe Massó Héctor González-Pacheco Julio Sandoval Carlos Guerrero Jorge Cervantes-Salazar José A. García-Montes Araceli Paéz Gabriela I. Pereira-López Carlos Zabal-Cerdeira Juan Pablo Sandoval |
author_sort | Juan Calderón-Colmenero |
collection | DOAJ |
description | Endothelial progenitor cells and circulating endothelial cells have been proposed as useful markers of severity and disease progression in certain vascular diseases, including pulmonary arterial hypertension. Our study focused on evaluating the levels of circulating endothelial progenitor cells and circulating endothelial cells in patients with congenital left-to-right shunts and pulmonary hypertension undergoing definitive repair. Endothelial progenitor cells (identified by simultaneous co-expression of CD45dim, CD34 + and KDR2 + surface antibodies) and circulating endothelial cells (identified by simultaneous co-expression of inherent antibodies CD45-, CD31+, CD146 + and CD105+) were prospectively measured in seventy-four children (including children with Down syndrome), median age six years (2.75–10), with clinically significant left-to-right shunts undergoing transcatheter or surgical repair and compared to thirty healthy controls. Endothelial progenitor cells and, particularly, circulating endothelial cells were significantly higher in children with heart disease and pulmonary arterial hypertension when compared to controls. Endothelial progenitor cells showed significant correlation with pulmonary vascular resistance index when measured both systemically (r = 0.259; p = 0.026) and in the superior vena cava (r = 0.302; p = 0.009). Children with Down syndrome showed a stronger correlation between systemic cellularity and pulmonary vascular resistance index (r = 0.829; p = 0.002). Endothelial progenitor cells were reduced along their transit through the lung, whereas circulating endothelial cells did not suffer any modification across the pulmonary circulation. In children with yet to be repaired left-to-right shunts, endothelial progenitor cells and circulating endothelial cell counts are increased compared to healthy subjects. |
first_indexed | 2024-03-13T01:02:39Z |
format | Article |
id | doaj.art-0f6494df565347cca29675cf786e4963 |
institution | Directory Open Access Journal |
issn | 2296-2360 |
language | English |
last_indexed | 2024-03-13T01:02:39Z |
publishDate | 2023-07-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Pediatrics |
spelling | doaj.art-0f6494df565347cca29675cf786e49632023-07-06T09:50:56ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602023-07-011110.3389/fped.2023.12003951200395Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severityJuan Calderón-Colmenero0Felipe Massó1Héctor González-Pacheco2Julio Sandoval3Carlos Guerrero4Jorge Cervantes-Salazar5José A. García-Montes6Araceli Paéz7Gabriela I. Pereira-López8Carlos Zabal-Cerdeira9Juan Pablo Sandoval10Department of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Molecular Biology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoCoronary Unit, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Cardiopulmonary Medicine, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Cardiovascular Surgery in Congenital Heart Disease, Instituto Nacional de Cardiologia Ignacio Chavez, MexicoCity, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Molecular Biology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoEndothelial progenitor cells and circulating endothelial cells have been proposed as useful markers of severity and disease progression in certain vascular diseases, including pulmonary arterial hypertension. Our study focused on evaluating the levels of circulating endothelial progenitor cells and circulating endothelial cells in patients with congenital left-to-right shunts and pulmonary hypertension undergoing definitive repair. Endothelial progenitor cells (identified by simultaneous co-expression of CD45dim, CD34 + and KDR2 + surface antibodies) and circulating endothelial cells (identified by simultaneous co-expression of inherent antibodies CD45-, CD31+, CD146 + and CD105+) were prospectively measured in seventy-four children (including children with Down syndrome), median age six years (2.75–10), with clinically significant left-to-right shunts undergoing transcatheter or surgical repair and compared to thirty healthy controls. Endothelial progenitor cells and, particularly, circulating endothelial cells were significantly higher in children with heart disease and pulmonary arterial hypertension when compared to controls. Endothelial progenitor cells showed significant correlation with pulmonary vascular resistance index when measured both systemically (r = 0.259; p = 0.026) and in the superior vena cava (r = 0.302; p = 0.009). Children with Down syndrome showed a stronger correlation between systemic cellularity and pulmonary vascular resistance index (r = 0.829; p = 0.002). Endothelial progenitor cells were reduced along their transit through the lung, whereas circulating endothelial cells did not suffer any modification across the pulmonary circulation. In children with yet to be repaired left-to-right shunts, endothelial progenitor cells and circulating endothelial cell counts are increased compared to healthy subjects.https://www.frontiersin.org/articles/10.3389/fped.2023.1200395/fullcirculating endothelial cellsendothelial progenitor cellspulmonary arterial hypertensioncongenital heart diseasedown syndrome |
spellingShingle | Juan Calderón-Colmenero Felipe Massó Héctor González-Pacheco Julio Sandoval Carlos Guerrero Jorge Cervantes-Salazar José A. García-Montes Araceli Paéz Gabriela I. Pereira-López Carlos Zabal-Cerdeira Juan Pablo Sandoval Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity Frontiers in Pediatrics circulating endothelial cells endothelial progenitor cells pulmonary arterial hypertension congenital heart disease down syndrome |
title | Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity |
title_full | Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity |
title_fullStr | Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity |
title_full_unstemmed | Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity |
title_short | Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity |
title_sort | pulmonary arterial hypertension in children with congenital heart disease a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity |
topic | circulating endothelial cells endothelial progenitor cells pulmonary arterial hypertension congenital heart disease down syndrome |
url | https://www.frontiersin.org/articles/10.3389/fped.2023.1200395/full |
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