Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity

Endothelial progenitor cells and circulating endothelial cells have been proposed as useful markers of severity and disease progression in certain vascular diseases, including pulmonary arterial hypertension. Our study focused on evaluating the levels of circulating endothelial progenitor cells and...

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Main Authors: Juan Calderón-Colmenero, Felipe Massó, Héctor González-Pacheco, Julio Sandoval, Carlos Guerrero, Jorge Cervantes-Salazar, José A. García-Montes, Araceli Paéz, Gabriela I. Pereira-López, Carlos Zabal-Cerdeira, Juan Pablo Sandoval
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-07-01
Series:Frontiers in Pediatrics
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fped.2023.1200395/full
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author Juan Calderón-Colmenero
Felipe Massó
Héctor González-Pacheco
Julio Sandoval
Carlos Guerrero
Jorge Cervantes-Salazar
José A. García-Montes
Araceli Paéz
Gabriela I. Pereira-López
Carlos Zabal-Cerdeira
Juan Pablo Sandoval
author_facet Juan Calderón-Colmenero
Felipe Massó
Héctor González-Pacheco
Julio Sandoval
Carlos Guerrero
Jorge Cervantes-Salazar
José A. García-Montes
Araceli Paéz
Gabriela I. Pereira-López
Carlos Zabal-Cerdeira
Juan Pablo Sandoval
author_sort Juan Calderón-Colmenero
collection DOAJ
description Endothelial progenitor cells and circulating endothelial cells have been proposed as useful markers of severity and disease progression in certain vascular diseases, including pulmonary arterial hypertension. Our study focused on evaluating the levels of circulating endothelial progenitor cells and circulating endothelial cells in patients with congenital left-to-right shunts and pulmonary hypertension undergoing definitive repair. Endothelial progenitor cells (identified by simultaneous co-expression of CD45dim, CD34 + and KDR2 + surface antibodies) and circulating endothelial cells (identified by simultaneous co-expression of inherent antibodies CD45-, CD31+, CD146 + and CD105+) were prospectively measured in seventy-four children (including children with Down syndrome), median age six years (2.75–10), with clinically significant left-to-right shunts undergoing transcatheter or surgical repair and compared to thirty healthy controls. Endothelial progenitor cells and, particularly, circulating endothelial cells were significantly higher in children with heart disease and pulmonary arterial hypertension when compared to controls. Endothelial progenitor cells showed significant correlation with pulmonary vascular resistance index when measured both systemically (r = 0.259; p = 0.026) and in the superior vena cava (r = 0.302; p = 0.009). Children with Down syndrome showed a stronger correlation between systemic cellularity and pulmonary vascular resistance index (r = 0.829; p = 0.002). Endothelial progenitor cells were reduced along their transit through the lung, whereas circulating endothelial cells did not suffer any modification across the pulmonary circulation. In children with yet to be repaired left-to-right shunts, endothelial progenitor cells and circulating endothelial cell counts are increased compared to healthy subjects.
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spelling doaj.art-0f6494df565347cca29675cf786e49632023-07-06T09:50:56ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602023-07-011110.3389/fped.2023.12003951200395Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severityJuan Calderón-Colmenero0Felipe Massó1Héctor González-Pacheco2Julio Sandoval3Carlos Guerrero4Jorge Cervantes-Salazar5José A. García-Montes6Araceli Paéz7Gabriela I. Pereira-López8Carlos Zabal-Cerdeira9Juan Pablo Sandoval10Department of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Molecular Biology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoCoronary Unit, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Cardiopulmonary Medicine, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Cardiovascular Surgery in Congenital Heart Disease, Instituto Nacional de Cardiologia Ignacio Chavez, MexicoCity, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Molecular Biology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoDepartment of Pediatric Cardiology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico City, MexicoEndothelial progenitor cells and circulating endothelial cells have been proposed as useful markers of severity and disease progression in certain vascular diseases, including pulmonary arterial hypertension. Our study focused on evaluating the levels of circulating endothelial progenitor cells and circulating endothelial cells in patients with congenital left-to-right shunts and pulmonary hypertension undergoing definitive repair. Endothelial progenitor cells (identified by simultaneous co-expression of CD45dim, CD34 + and KDR2 + surface antibodies) and circulating endothelial cells (identified by simultaneous co-expression of inherent antibodies CD45-, CD31+, CD146 + and CD105+) were prospectively measured in seventy-four children (including children with Down syndrome), median age six years (2.75–10), with clinically significant left-to-right shunts undergoing transcatheter or surgical repair and compared to thirty healthy controls. Endothelial progenitor cells and, particularly, circulating endothelial cells were significantly higher in children with heart disease and pulmonary arterial hypertension when compared to controls. Endothelial progenitor cells showed significant correlation with pulmonary vascular resistance index when measured both systemically (r = 0.259; p = 0.026) and in the superior vena cava (r = 0.302; p = 0.009). Children with Down syndrome showed a stronger correlation between systemic cellularity and pulmonary vascular resistance index (r = 0.829; p = 0.002). Endothelial progenitor cells were reduced along their transit through the lung, whereas circulating endothelial cells did not suffer any modification across the pulmonary circulation. In children with yet to be repaired left-to-right shunts, endothelial progenitor cells and circulating endothelial cell counts are increased compared to healthy subjects.https://www.frontiersin.org/articles/10.3389/fped.2023.1200395/fullcirculating endothelial cellsendothelial progenitor cellspulmonary arterial hypertensioncongenital heart diseasedown syndrome
spellingShingle Juan Calderón-Colmenero
Felipe Massó
Héctor González-Pacheco
Julio Sandoval
Carlos Guerrero
Jorge Cervantes-Salazar
José A. García-Montes
Araceli Paéz
Gabriela I. Pereira-López
Carlos Zabal-Cerdeira
Juan Pablo Sandoval
Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity
Frontiers in Pediatrics
circulating endothelial cells
endothelial progenitor cells
pulmonary arterial hypertension
congenital heart disease
down syndrome
title Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity
title_full Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity
title_fullStr Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity
title_full_unstemmed Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity
title_short Pulmonary arterial hypertension in children with congenital heart disease: a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity
title_sort pulmonary arterial hypertension in children with congenital heart disease a deeper look into the role of endothelial progenitor cells and circulating endothelial cells to assess disease severity
topic circulating endothelial cells
endothelial progenitor cells
pulmonary arterial hypertension
congenital heart disease
down syndrome
url https://www.frontiersin.org/articles/10.3389/fped.2023.1200395/full
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