Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines
Ejaculates and blood plasma were sampled from cocks of three laying lines: Barred Plymouth Rock (BPR), Sussex Light (SU), and Rhode Island Red (RIR). Ejaculates and blood plasma were sampled four times during the laying period of hens. The following ejaculate parameters were determined: sperm motili...
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Czech Academy of Agricultural Sciences
2012-08-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201208-0004_relationship-of-ejaculate-quality-and-selected-biochemical-parameters-of-blood-in-cockerels-of-three-laying-lin.php |
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| author | L. Jarinkovičová L. Máchal J. Máchal R. Filipčík E. Tůmová R. Horský |
| author_facet | L. Jarinkovičová L. Máchal J. Máchal R. Filipčík E. Tůmová R. Horský |
| author_sort | L. Jarinkovičová |
| collection | DOAJ |
| description | Ejaculates and blood plasma were sampled from cocks of three laying lines: Barred Plymouth Rock (BPR), Sussex Light (SU), and Rhode Island Red (RIR). Ejaculates and blood plasma were sampled four times during the laying period of hens. The following ejaculate parameters were determined: sperm motility, concentrations of sperm cells, ejaculate volume. Sperm morphology was examined. In the blood samples, concentrations of glucose, cholesterol, creatinine, calcium, phosphorus, and magnesium were analysed. The motility of spermatozoa of the cocks was 47.0% (BPR), 47.7% (RIR), and 48.3% (SU), respectively. The highest volume of ejaculate was found in BPR line (0.66 cm3), the lowest one in SU line (0.46 cm3, P ≤ 0.01); the highest sperm concentration was in SU line (2.46 × 106/mm3), the lowest one in RIR line (1.96 × 106/mm3, P ≤ 0.01). The number of morphologically abnormal sperm cells was similar in all lines - 47.0% BPR, 47.7% RIR, and 48.3% SU, respectively. In general, the occurrence of defective spermatozoa was high in all three lines; the most frequent were tail defects (from 20.3 to 29.7%), while sperm cells with developmental anomalies were less frequent (only 0.3 to 0.4%). Calculated phenotypic correlation between sperm motility on the one hand and the occurrence of defective sperm cells on the other was negative (rp = -0.28, P ≤ 0.01), as well as the correlation between sperm motility and sperm numbers, and between sperm motility and ejaculate volume (rp = -0.28, P ≤ 0.01 and rp = -0.31, P ≤ 0.01, respectively). Negative correlations were found between the level of magnesium in blood plasma and numbers of morphologically defective spermatozoa in the ejaculate, defective heads and defective connecting pieces (rp = -0.33, P ≤ 0.01; rp = -0.23, P ≤ 0.05; and rp = -0.26, P ≤ 0.05). Level of magnesium was positively correlated to sperm motility (rp = 0.26, P ≤ 0.05). However, positive correlations existed between concentration of glucose in blood plasma of cocks and numbers of morphologically defective spermatozoa in sampled ejaculates (rp = 0.27, P ≤ 0.01). Our finding is in accordance with the results found in mammals and other animals, and it shows an important role of magnesium as a key contributor to the quality of ejaculate in aviary species, in our case in laying lines of domestic fowl (Gallus domesticus). |
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| spelling | doaj.art-0f64b449d6fb41b8b19c264e6cff91f32023-02-23T03:33:00ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092012-08-0157837037610.17221/6272-CJAScjs-201208-0004Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying linesL. Jarinkovičová0L. Máchal1J. Máchal2R. Filipčík3E. Tůmová4R. Horský5Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicFaculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech RepublicFaculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicFaculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicFaculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicEjaculates and blood plasma were sampled from cocks of three laying lines: Barred Plymouth Rock (BPR), Sussex Light (SU), and Rhode Island Red (RIR). Ejaculates and blood plasma were sampled four times during the laying period of hens. The following ejaculate parameters were determined: sperm motility, concentrations of sperm cells, ejaculate volume. Sperm morphology was examined. In the blood samples, concentrations of glucose, cholesterol, creatinine, calcium, phosphorus, and magnesium were analysed. The motility of spermatozoa of the cocks was 47.0% (BPR), 47.7% (RIR), and 48.3% (SU), respectively. The highest volume of ejaculate was found in BPR line (0.66 cm3), the lowest one in SU line (0.46 cm3, P ≤ 0.01); the highest sperm concentration was in SU line (2.46 × 106/mm3), the lowest one in RIR line (1.96 × 106/mm3, P ≤ 0.01). The number of morphologically abnormal sperm cells was similar in all lines - 47.0% BPR, 47.7% RIR, and 48.3% SU, respectively. In general, the occurrence of defective spermatozoa was high in all three lines; the most frequent were tail defects (from 20.3 to 29.7%), while sperm cells with developmental anomalies were less frequent (only 0.3 to 0.4%). Calculated phenotypic correlation between sperm motility on the one hand and the occurrence of defective sperm cells on the other was negative (rp = -0.28, P ≤ 0.01), as well as the correlation between sperm motility and sperm numbers, and between sperm motility and ejaculate volume (rp = -0.28, P ≤ 0.01 and rp = -0.31, P ≤ 0.01, respectively). Negative correlations were found between the level of magnesium in blood plasma and numbers of morphologically defective spermatozoa in the ejaculate, defective heads and defective connecting pieces (rp = -0.33, P ≤ 0.01; rp = -0.23, P ≤ 0.05; and rp = -0.26, P ≤ 0.05). Level of magnesium was positively correlated to sperm motility (rp = 0.26, P ≤ 0.05). However, positive correlations existed between concentration of glucose in blood plasma of cocks and numbers of morphologically defective spermatozoa in sampled ejaculates (rp = 0.27, P ≤ 0.01). Our finding is in accordance with the results found in mammals and other animals, and it shows an important role of magnesium as a key contributor to the quality of ejaculate in aviary species, in our case in laying lines of domestic fowl (Gallus domesticus).https://cjas.agriculturejournals.cz/artkey/cjs-201208-0004_relationship-of-ejaculate-quality-and-selected-biochemical-parameters-of-blood-in-cockerels-of-three-laying-lin.phpcocksejaculateblood plasmapathological spermsglucosemagnesiumbiochemical criteria |
| spellingShingle | L. Jarinkovičová L. Máchal J. Máchal R. Filipčík E. Tůmová R. Horský Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines Czech Journal of Animal Science cocks ejaculate blood plasma pathological sperms glucose magnesium biochemical criteria |
| title | Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines |
| title_full | Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines |
| title_fullStr | Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines |
| title_full_unstemmed | Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines |
| title_short | Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines |
| title_sort | relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines |
| topic | cocks ejaculate blood plasma pathological sperms glucose magnesium biochemical criteria |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201208-0004_relationship-of-ejaculate-quality-and-selected-biochemical-parameters-of-blood-in-cockerels-of-three-laying-lin.php |
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