Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.)
Abstract Cold temperatures can be detrimental to crop survival and productivity. Breeding progress can be improved by understanding the molecular basis of low temperature tolerance. We investigated the key routes and critical metabolites related to low temperature resistance in cold-tolerant and -se...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04094-1 |
| _version_ | 1811165975855235072 |
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| author | Xiaoxu Yang Chang Liu Mengdi Li Yanmei Li Zhishan Yan Guojun Feng Dajun Liu |
| author_facet | Xiaoxu Yang Chang Liu Mengdi Li Yanmei Li Zhishan Yan Guojun Feng Dajun Liu |
| author_sort | Xiaoxu Yang |
| collection | DOAJ |
| description | Abstract Cold temperatures can be detrimental to crop survival and productivity. Breeding progress can be improved by understanding the molecular basis of low temperature tolerance. We investigated the key routes and critical metabolites related to low temperature resistance in cold-tolerant and -sensitive common bean cultivars 120 and 093, respectively. Many potential genes and metabolites implicated in major metabolic pathways during the chilling stress response were identified through transcriptomics and metabolomics research. Under chilling stress, the expression of many genes involved in lipid, amino acid, and flavonoid metabolism, as well as metabolite accumulation increased in the two bean types. Malondialdehyde (MDA) content was lower in 120 than in 093. Regarding amino acid metabolism, 120 had a higher concentration of acidic amino acids than 093, whereas 093 had a higher concentration of basic amino acids. Methionine accumulation was clearly higher in 120 than in 093. In addition, 120 had a higher concentration of many types of flavonoids than 093. Flavonoids, methionine and malondialdehyde could be used as biomarkers of plant chilling injury. Transcriptome analysis of hormone metabolism revealed considerably greater, expression of abscisic acid (ABA), gibberellin (GA), and jasmonic acid (JA) in 093 than in 120 during chilling stress, indicating that hormone regulation modes in 093 and 120 were different. Thus, chilling stress tolerance is different between 093 and 120 possibly due to transcriptional and metabolic regulation. |
| first_indexed | 2024-04-10T15:44:55Z |
| format | Article |
| id | doaj.art-0f65adb1ce3e4a209a56e4c51294ffd8 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-10T15:44:55Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-0f65adb1ce3e4a209a56e4c51294ffd82023-02-12T12:08:21ZengBMCBMC Plant Biology1471-22292023-02-0123111810.1186/s12870-023-04094-1Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.)Xiaoxu Yang0Chang Liu1Mengdi Li2Yanmei Li3Zhishan Yan4Guojun Feng5Dajun Liu6Horticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Life Sciences, Heilongjiang UniversityHorticulture Department, College of Life Sciences, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityAbstract Cold temperatures can be detrimental to crop survival and productivity. Breeding progress can be improved by understanding the molecular basis of low temperature tolerance. We investigated the key routes and critical metabolites related to low temperature resistance in cold-tolerant and -sensitive common bean cultivars 120 and 093, respectively. Many potential genes and metabolites implicated in major metabolic pathways during the chilling stress response were identified through transcriptomics and metabolomics research. Under chilling stress, the expression of many genes involved in lipid, amino acid, and flavonoid metabolism, as well as metabolite accumulation increased in the two bean types. Malondialdehyde (MDA) content was lower in 120 than in 093. Regarding amino acid metabolism, 120 had a higher concentration of acidic amino acids than 093, whereas 093 had a higher concentration of basic amino acids. Methionine accumulation was clearly higher in 120 than in 093. In addition, 120 had a higher concentration of many types of flavonoids than 093. Flavonoids, methionine and malondialdehyde could be used as biomarkers of plant chilling injury. Transcriptome analysis of hormone metabolism revealed considerably greater, expression of abscisic acid (ABA), gibberellin (GA), and jasmonic acid (JA) in 093 than in 120 during chilling stress, indicating that hormone regulation modes in 093 and 120 were different. Thus, chilling stress tolerance is different between 093 and 120 possibly due to transcriptional and metabolic regulation.https://doi.org/10.1186/s12870-023-04094-1Common beanCold stressTranscriptomicsMetabolomicsRegulatory network |
| spellingShingle | Xiaoxu Yang Chang Liu Mengdi Li Yanmei Li Zhishan Yan Guojun Feng Dajun Liu Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.) BMC Plant Biology Common bean Cold stress Transcriptomics Metabolomics Regulatory network |
| title | Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.) |
| title_full | Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.) |
| title_fullStr | Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.) |
| title_full_unstemmed | Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.) |
| title_short | Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.) |
| title_sort | integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common bean phaseolus vulgaris l |
| topic | Common bean Cold stress Transcriptomics Metabolomics Regulatory network |
| url | https://doi.org/10.1186/s12870-023-04094-1 |
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