M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A.
The ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogeni...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3601086?pdf=render |
| _version_ | 1818542897825316864 |
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| author | Lorena Itatí Ibañez Kenny Roose Marina De Filette Michael Schotsaert Jessica De Sloovere Stefan Roels Charlotte Pollard Bert Schepens Johan Grooten Walter Fiers Xavier Saelens |
| author_facet | Lorena Itatí Ibañez Kenny Roose Marina De Filette Michael Schotsaert Jessica De Sloovere Stefan Roels Charlotte Pollard Bert Schepens Johan Grooten Walter Fiers Xavier Saelens |
| author_sort | Lorena Itatí Ibañez |
| collection | DOAJ |
| description | The ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogenicity and protective efficacy. Immunization of BALB/c mice with M2e-VLPs containing nucleic acids induced a stronger, Th1-biased antibody response compared to particles lacking nucleic acids. The former also induced a stronger M2e-specific CD4(+) T cell response, as determined by ELISPOT. Mice vaccinated with alum-adjuvanted M2e-VLPs containing the nucleic acid-binding domain were better protected against influenza A virus challenge than mice vaccinated with similar particles lacking this domain, as deduced from the loss in body weight following challenge with X47 (H3N2) or PR/8 virus. Challenge of mice that had been immunized with M2e-VLPs with or without nucleic acids displayed significantly lower mortality, morbidity and lung virus titers than control-immunized groups. We conclude that nucleic acids present in M2e-VLPs correlate with improved immune protection. |
| first_indexed | 2024-12-11T22:28:12Z |
| format | Article |
| id | doaj.art-0f684242583f4809934234a2419d80f8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T22:28:12Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0f684242583f4809934234a2419d80f82022-12-22T00:48:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5908110.1371/journal.pone.0059081M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A.Lorena Itatí IbañezKenny RooseMarina De FiletteMichael SchotsaertJessica De SloovereStefan RoelsCharlotte PollardBert SchepensJohan GrootenWalter FiersXavier SaelensThe ectodomain of influenza A matrix protein 2 (M2e) is a candidate for a universal influenza A vaccine. We used recombinant Hepatitis B core antigen to produce virus-like particles presenting M2e (M2e-VLPs). We produced the VLPs with and without entrapped nucleic acids and compared their immunogenicity and protective efficacy. Immunization of BALB/c mice with M2e-VLPs containing nucleic acids induced a stronger, Th1-biased antibody response compared to particles lacking nucleic acids. The former also induced a stronger M2e-specific CD4(+) T cell response, as determined by ELISPOT. Mice vaccinated with alum-adjuvanted M2e-VLPs containing the nucleic acid-binding domain were better protected against influenza A virus challenge than mice vaccinated with similar particles lacking this domain, as deduced from the loss in body weight following challenge with X47 (H3N2) or PR/8 virus. Challenge of mice that had been immunized with M2e-VLPs with or without nucleic acids displayed significantly lower mortality, morbidity and lung virus titers than control-immunized groups. We conclude that nucleic acids present in M2e-VLPs correlate with improved immune protection.http://europepmc.org/articles/PMC3601086?pdf=render |
| spellingShingle | Lorena Itatí Ibañez Kenny Roose Marina De Filette Michael Schotsaert Jessica De Sloovere Stefan Roels Charlotte Pollard Bert Schepens Johan Grooten Walter Fiers Xavier Saelens M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A. PLoS ONE |
| title | M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A. |
| title_full | M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A. |
| title_fullStr | M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A. |
| title_full_unstemmed | M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A. |
| title_short | M2e-displaying virus-like particles with associated RNA promote T helper 1 type adaptive immunity against influenza A. |
| title_sort | m2e displaying virus like particles with associated rna promote t helper 1 type adaptive immunity against influenza a |
| url | http://europepmc.org/articles/PMC3601086?pdf=render |
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