Total HIV DNA: a global marker of HIV persistence
Abstract Among the different markers of HIV persistence in infected cells, total HIV DNA is to date the most widely used. It allows an overall quantification of all viral forms of HIV DNA in infected cells, each playing a different role in HIV replication and pathophysiology. The real-time PCR techn...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Retrovirology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12977-018-0412-7 |
| _version_ | 1818766536956968960 |
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| author | Christine Rouzioux Véronique Avettand-Fenoël |
| author_facet | Christine Rouzioux Véronique Avettand-Fenoël |
| author_sort | Christine Rouzioux |
| collection | DOAJ |
| description | Abstract Among the different markers of HIV persistence in infected cells, total HIV DNA is to date the most widely used. It allows an overall quantification of all viral forms of HIV DNA in infected cells, each playing a different role in HIV replication and pathophysiology. The real-time PCR technology is to date, a precise, sensitive and reproducible technology that allows the description of the distribution of HIV infected cells in blood and tissues. The objective of this review is to present some examples which show the interest to quantify total HIV DNA levels. This marker brought an undeniable and considerable contribution to reservoir studies. Many results, both in clinical and basic research, allowed to get a large overview of the distribution of infected cells in the body, at all stages of HIV disease and during therapy. Future clinical studies aiming at reducing HIV reservoirs will benefit from HIV DNA quantification in blood and tissues, in association with other markers of HIV reservoir activity. |
| first_indexed | 2024-12-18T08:35:33Z |
| format | Article |
| id | doaj.art-0f6874f1b618469c9c55aa6b46530b9b |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-12-18T08:35:33Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-0f6874f1b618469c9c55aa6b46530b9b2022-12-21T21:14:19ZengBMCRetrovirology1742-46902018-04-011511710.1186/s12977-018-0412-7Total HIV DNA: a global marker of HIV persistenceChristine Rouzioux0Véronique Avettand-Fenoël1Laboratoire de Virologie, APHP Hôpital Necker Enfants MaladesLaboratoire de Virologie, APHP Hôpital Necker Enfants MaladesAbstract Among the different markers of HIV persistence in infected cells, total HIV DNA is to date the most widely used. It allows an overall quantification of all viral forms of HIV DNA in infected cells, each playing a different role in HIV replication and pathophysiology. The real-time PCR technology is to date, a precise, sensitive and reproducible technology that allows the description of the distribution of HIV infected cells in blood and tissues. The objective of this review is to present some examples which show the interest to quantify total HIV DNA levels. This marker brought an undeniable and considerable contribution to reservoir studies. Many results, both in clinical and basic research, allowed to get a large overview of the distribution of infected cells in the body, at all stages of HIV disease and during therapy. Future clinical studies aiming at reducing HIV reservoirs will benefit from HIV DNA quantification in blood and tissues, in association with other markers of HIV reservoir activity.http://link.springer.com/article/10.1186/s12977-018-0412-7Total HIV DNAReal time PCRReservoirsCD4 + T cell subsets |
| spellingShingle | Christine Rouzioux Véronique Avettand-Fenoël Total HIV DNA: a global marker of HIV persistence Retrovirology Total HIV DNA Real time PCR Reservoirs CD4 + T cell subsets |
| title | Total HIV DNA: a global marker of HIV persistence |
| title_full | Total HIV DNA: a global marker of HIV persistence |
| title_fullStr | Total HIV DNA: a global marker of HIV persistence |
| title_full_unstemmed | Total HIV DNA: a global marker of HIV persistence |
| title_short | Total HIV DNA: a global marker of HIV persistence |
| title_sort | total hiv dna a global marker of hiv persistence |
| topic | Total HIV DNA Real time PCR Reservoirs CD4 + T cell subsets |
| url | http://link.springer.com/article/10.1186/s12977-018-0412-7 |
| work_keys_str_mv | AT christinerouzioux totalhivdnaaglobalmarkerofhivpersistence AT veroniqueavettandfenoel totalhivdnaaglobalmarkerofhivpersistence |