The Effects of Vitex agnus castus Total Extract on Spermatogenesis of Balb/C Mice
ABSTRACT Introduction...
| Main Authors: | , , |
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| Format: | Article |
| Language: | fas |
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Yasuj University Of Medical Sciences
2008-12-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-381&slc_lang=en&sid=1 |
| _version_ | 1818024841195814912 |
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| author | M Ramezani S Nasri H Bahadoran |
| author_facet | M Ramezani S Nasri H Bahadoran |
| author_sort | M Ramezani |
| collection | DOAJ |
| description | ABSTRACT
Introduction & Objective: Vitex agnus castus (Verbenaceae) is a phytoestrogeic herb native to the Middle East and southern Europe. It has clinical usage in so many countries. In this research, the effects of Vitex agnus castus extract was investigated on spermatogenesis of male Balb/C mice.
Materials & Methods: This is an experimental study in which adult male mice were chosen and divided into 3 groups: control, vehicle, and experimental. Animals were daily injected (i.p.) with 65, 165 265, 365, and 465 mg/kg of seed extract for ten consecutive days. Then the animals were weighed and eventually killed by cervical dislocation 2 weeks after the last injection. The caudal part of the right epididymis was used for sperm counting. After macroscopic investigation (weight, diameter and volume of testes) tissues were fixed in Buin's fixative. Tissues were cut at 5 µm, stained with Hematoxylin and Eosin (H& E).Ccollected data was analyzed by the SPSS software by using one-way ANOVA.
Results: No significant differences in body weight, volume, weight and diameter of testes was seen. Light microscopic studies showed a significant reduction in germinal epithelium in doses of 265 and 365 mg/kg and increased of interstitial tissue area in doses of 265, 365, and 465 mg/kg of extract. There was no significant difference in epithelium thickness and of the diameter of the epididymis. Germinal cells contained pyknotic nuclei and several holes that were found scattered in the tubules. Testis also showed a general disarrangement in various germinal elements of seminiferous tubules. Result of sperms count indicated a significant decreasing of spermatozoa in animals which received 265 and 365 mg/kg of extract.
Conclusion: Vitex agnus castus contains essential oils, iridoid glycosides, flavonoids diterpenes, and essential fatty acids. The results suggest that its contraceptive effects is related to its flavonoids and essential fatty acids but further studies is needed to focus on the pharmacokinetics of this plant. |
| first_indexed | 2024-12-10T04:06:37Z |
| format | Article |
| id | doaj.art-0f68ca572c6d4361b0b4f64a0a84f3fb |
| institution | Directory Open Access Journal |
| issn | 1728-6506 1728-6514 |
| language | fas |
| last_indexed | 2024-12-10T04:06:37Z |
| publishDate | 2008-12-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-0f68ca572c6d4361b0b4f64a0a84f3fb2022-12-22T02:02:49ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65061728-65142008-12-011333544The Effects of Vitex agnus castus Total Extract on Spermatogenesis of Balb/C MiceM Ramezani0S Nasri1H Bahadoran2 ABSTRACT Introduction & Objective: Vitex agnus castus (Verbenaceae) is a phytoestrogeic herb native to the Middle East and southern Europe. It has clinical usage in so many countries. In this research, the effects of Vitex agnus castus extract was investigated on spermatogenesis of male Balb/C mice. Materials & Methods: This is an experimental study in which adult male mice were chosen and divided into 3 groups: control, vehicle, and experimental. Animals were daily injected (i.p.) with 65, 165 265, 365, and 465 mg/kg of seed extract for ten consecutive days. Then the animals were weighed and eventually killed by cervical dislocation 2 weeks after the last injection. The caudal part of the right epididymis was used for sperm counting. After macroscopic investigation (weight, diameter and volume of testes) tissues were fixed in Buin's fixative. Tissues were cut at 5 µm, stained with Hematoxylin and Eosin (H& E).Ccollected data was analyzed by the SPSS software by using one-way ANOVA. Results: No significant differences in body weight, volume, weight and diameter of testes was seen. Light microscopic studies showed a significant reduction in germinal epithelium in doses of 265 and 365 mg/kg and increased of interstitial tissue area in doses of 265, 365, and 465 mg/kg of extract. There was no significant difference in epithelium thickness and of the diameter of the epididymis. Germinal cells contained pyknotic nuclei and several holes that were found scattered in the tubules. Testis also showed a general disarrangement in various germinal elements of seminiferous tubules. Result of sperms count indicated a significant decreasing of spermatozoa in animals which received 265 and 365 mg/kg of extract. Conclusion: Vitex agnus castus contains essential oils, iridoid glycosides, flavonoids diterpenes, and essential fatty acids. The results suggest that its contraceptive effects is related to its flavonoids and essential fatty acids but further studies is needed to focus on the pharmacokinetics of this plant.http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-381&slc_lang=en&sid=1KEYWORDS: Vitex agnus castus Male Gonad Spermatogenesis |
| spellingShingle | M Ramezani S Nasri H Bahadoran The Effects of Vitex agnus castus Total Extract on Spermatogenesis of Balb/C Mice Armaghane Danesh Bimonthly Journal KEYWORDS: Vitex agnus castus Male Gonad Spermatogenesis |
| title | The Effects of Vitex agnus castus Total Extract on Spermatogenesis of Balb/C Mice |
| title_full | The Effects of Vitex agnus castus Total Extract on Spermatogenesis of Balb/C Mice |
| title_fullStr | The Effects of Vitex agnus castus Total Extract on Spermatogenesis of Balb/C Mice |
| title_full_unstemmed | The Effects of Vitex agnus castus Total Extract on Spermatogenesis of Balb/C Mice |
| title_short | The Effects of Vitex agnus castus Total Extract on Spermatogenesis of Balb/C Mice |
| title_sort | effects of vitex agnus castus total extract on spermatogenesis of balb c mice |
| topic | KEYWORDS: Vitex agnus castus Male Gonad Spermatogenesis |
| url | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-381&slc_lang=en&sid=1 |
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