Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases
In this study, we focus on a detailed bioinformatics analysis of <i>hyBpox</i> genes, mainly within the genomes of <i>Sclerotiniaceae</i> (Ascomycota, Leotiomycetes), which is a specifically evolved fungal family of necrotrophic host generalists and saprophytic or biotrophic...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/3/459 |
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| author | Marcel Zámocký Miloš Musil Maksym Danchenko Peter Ferianc Katarína Chovanová Peter Baráth Andrej Poljovka David Bednář |
| author_facet | Marcel Zámocký Miloš Musil Maksym Danchenko Peter Ferianc Katarína Chovanová Peter Baráth Andrej Poljovka David Bednář |
| author_sort | Marcel Zámocký |
| collection | DOAJ |
| description | In this study, we focus on a detailed bioinformatics analysis of <i>hyBpox</i> genes, mainly within the genomes of <i>Sclerotiniaceae</i> (Ascomycota, Leotiomycetes), which is a specifically evolved fungal family of necrotrophic host generalists and saprophytic or biotrophic host specialists. Members of the genus <i>Sclerotium</i> produce only sclerotia and no fruiting bodies or spores. Thus, their physiological role for peroxidases remains open. A representative species, <i>S. cepivorum,</i> is a dangerous plant pathogen causing white rot in <i>Allium</i> species, particularly in onions, leeks, and garlic. On a worldwide basis, the white rot caused by this soil-borne fungus is apparently the most serious threat to <i>Allium</i>-crop production. We have also found very similar peroxidase sequences in the related fungus <i>S. sclerotiorum</i>, although with minor yet important modifications in the architecture of its active centre. The presence of <i>ScephyBpox1-</i>specific mRNA was confirmed by transcriptomic analysis. The presence of Hybrid B peroxidase at the protein level as the sole extracellular peroxidase of this fungus was confirmed in the secretome of <i>S. cepivorum</i> through detailed proteomic analyses. This prompted us to systematically search for all available genes coding for Hybrid B heme peroxidases in the whole fungal family of <i>Sclerotiniaceae</i>. We present here a reconstruction of their molecular phylogeny and analyse the unique aspects of their conserved-sequence features and structural folds in corresponding ancestral sequences. |
| first_indexed | 2024-03-09T13:48:45Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T13:48:45Z |
| publishDate | 2022-03-01 |
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| series | Biology |
| spelling | doaj.art-0f69423f27c14a7bbc4d175d6b9e47ec2023-11-30T20:52:30ZengMDPI AGBiology2079-77372022-03-0111345910.3390/biology11030459Deep Insights into the Specific Evolution of Fungal Hybrid B Heme PeroxidasesMarcel Zámocký0Miloš Musil1Maksym Danchenko2Peter Ferianc3Katarína Chovanová4Peter Baráth5Andrej Poljovka6David Bednář7Laboratory for Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551 Bratislava, SlovakiaLoschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, CZ-61137 Brno, Czech RepublicDepartment of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, SlovakiaLaboratory for Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551 Bratislava, SlovakiaLaboratory for Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551 Bratislava, SlovakiaDepartment of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, SlovakiaLaboratory for Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551 Bratislava, SlovakiaLoschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, CZ-61137 Brno, Czech RepublicIn this study, we focus on a detailed bioinformatics analysis of <i>hyBpox</i> genes, mainly within the genomes of <i>Sclerotiniaceae</i> (Ascomycota, Leotiomycetes), which is a specifically evolved fungal family of necrotrophic host generalists and saprophytic or biotrophic host specialists. Members of the genus <i>Sclerotium</i> produce only sclerotia and no fruiting bodies or spores. Thus, their physiological role for peroxidases remains open. A representative species, <i>S. cepivorum,</i> is a dangerous plant pathogen causing white rot in <i>Allium</i> species, particularly in onions, leeks, and garlic. On a worldwide basis, the white rot caused by this soil-borne fungus is apparently the most serious threat to <i>Allium</i>-crop production. We have also found very similar peroxidase sequences in the related fungus <i>S. sclerotiorum</i>, although with minor yet important modifications in the architecture of its active centre. The presence of <i>ScephyBpox1-</i>specific mRNA was confirmed by transcriptomic analysis. The presence of Hybrid B peroxidase at the protein level as the sole extracellular peroxidase of this fungus was confirmed in the secretome of <i>S. cepivorum</i> through detailed proteomic analyses. This prompted us to systematically search for all available genes coding for Hybrid B heme peroxidases in the whole fungal family of <i>Sclerotiniaceae</i>. We present here a reconstruction of their molecular phylogeny and analyse the unique aspects of their conserved-sequence features and structural folds in corresponding ancestral sequences.https://www.mdpi.com/2079-7737/11/3/459hybrid B heme peroxidaseperoxidase–catalase superfamilyoxidative stressenzymatic antioxidantancestral sequence reconstruction |
| spellingShingle | Marcel Zámocký Miloš Musil Maksym Danchenko Peter Ferianc Katarína Chovanová Peter Baráth Andrej Poljovka David Bednář Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases Biology hybrid B heme peroxidase peroxidase–catalase superfamily oxidative stress enzymatic antioxidant ancestral sequence reconstruction |
| title | Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases |
| title_full | Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases |
| title_fullStr | Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases |
| title_full_unstemmed | Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases |
| title_short | Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases |
| title_sort | deep insights into the specific evolution of fungal hybrid b heme peroxidases |
| topic | hybrid B heme peroxidase peroxidase–catalase superfamily oxidative stress enzymatic antioxidant ancestral sequence reconstruction |
| url | https://www.mdpi.com/2079-7737/11/3/459 |
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