Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy
Mechanotransduction is an essential mechanism of transforming external mechanical stimulus to biochemical response. In cardiomyocytes mechanotransduction plays an important role in contraction, stretch sensing and homeostasis regulation. One of the major mechanosensitive area in cardiomyocytes, the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2018.00684/full |
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| author | Anastasia Knyazeva Alexander Krutikov Alexey Golovkin Alexander Mishanin Georgii Pavlov Natalia Smolina Natalia Smolina Anastasia Hushkina Thomas Sejersen Gunnar Sjoberg Mikhail Galagudza Anna Kostareva Anna Kostareva |
| author_facet | Anastasia Knyazeva Alexander Krutikov Alexey Golovkin Alexander Mishanin Georgii Pavlov Natalia Smolina Natalia Smolina Anastasia Hushkina Thomas Sejersen Gunnar Sjoberg Mikhail Galagudza Anna Kostareva Anna Kostareva |
| author_sort | Anastasia Knyazeva |
| collection | DOAJ |
| description | Mechanotransduction is an essential mechanism of transforming external mechanical stimulus to biochemical response. In cardiomyocytes mechanotransduction plays an important role in contraction, stretch sensing and homeostasis regulation. One of the major mechanosensitive area in cardiomyocytes, the Z-disk, consists of numbers of structural and signaling proteins, that may undergo conformational or gene expression changes under pathological stress conditions. In present study we examined a rat model of pressure overload cardiac hypertrophy validated by echocardiographic and histopathological examinations. We revealed, that during hypertrophy progression expression of several genes encoding Z-disk proteins (Actn2, Ldb3, Cmya5, Nebl) is different at early and late points of cardiac remodeling. Moreover, expression patterns of several genes are opposite in myocardium of overloaded left ventricle and hemodynamically unaffected right ventricle, and expression profiles in interventricular septum are more similar to right ventricle. Additionally, we revealed inconsistencies between mRNA and protein level changes of Actn2, one of the major structural Z-disk element. All these findings point out, that investigated Z-disk proteins participate in pathological stress adaptation through undergoing the gene expression changes, and suggest the novel important role of hypertrophic response modulation during different stages of cardiac remodeling. |
| first_indexed | 2024-12-21T09:29:09Z |
| format | Article |
| id | doaj.art-0f698ef063d84f8bbfd3b7803c3a117c |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-21T09:29:09Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-0f698ef063d84f8bbfd3b7803c3a117c2022-12-21T19:08:48ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-01-01910.3389/fgene.2018.00684428843Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular HypertrophyAnastasia Knyazeva0Alexander Krutikov1Alexey Golovkin2Alexander Mishanin3Georgii Pavlov4Natalia Smolina5Natalia Smolina6Anastasia Hushkina7Thomas Sejersen8Gunnar Sjoberg9Mikhail Galagudza10Anna Kostareva11Anna Kostareva12Almazov National Medical Research Centre, Saint Petersburg, RussiaAlmazov National Medical Research Centre, Saint Petersburg, RussiaAlmazov National Medical Research Centre, Saint Petersburg, RussiaAlmazov National Medical Research Centre, Saint Petersburg, RussiaSaint Petersburg State Academy of Veterinary Medicine, Saint Petersburg, RussiaAlmazov National Medical Research Centre, Saint Petersburg, RussiaInformation Technologies and Programming Faculty, ITMO University, Saint Petersburg, RussiaAlmazov National Medical Research Centre, Saint Petersburg, RussiaDepartment of Women’s and Children’s Health, Center for Molecular Medicine, Karolinska Institute, Stockholm, SwedenDepartment of Women’s and Children’s Health, Center for Molecular Medicine, Karolinska Institute, Stockholm, SwedenAlmazov National Medical Research Centre, Saint Petersburg, RussiaAlmazov National Medical Research Centre, Saint Petersburg, RussiaInformation Technologies and Programming Faculty, ITMO University, Saint Petersburg, RussiaMechanotransduction is an essential mechanism of transforming external mechanical stimulus to biochemical response. In cardiomyocytes mechanotransduction plays an important role in contraction, stretch sensing and homeostasis regulation. One of the major mechanosensitive area in cardiomyocytes, the Z-disk, consists of numbers of structural and signaling proteins, that may undergo conformational or gene expression changes under pathological stress conditions. In present study we examined a rat model of pressure overload cardiac hypertrophy validated by echocardiographic and histopathological examinations. We revealed, that during hypertrophy progression expression of several genes encoding Z-disk proteins (Actn2, Ldb3, Cmya5, Nebl) is different at early and late points of cardiac remodeling. Moreover, expression patterns of several genes are opposite in myocardium of overloaded left ventricle and hemodynamically unaffected right ventricle, and expression profiles in interventricular septum are more similar to right ventricle. Additionally, we revealed inconsistencies between mRNA and protein level changes of Actn2, one of the major structural Z-disk element. All these findings point out, that investigated Z-disk proteins participate in pathological stress adaptation through undergoing the gene expression changes, and suggest the novel important role of hypertrophic response modulation during different stages of cardiac remodeling.https://www.frontiersin.org/article/10.3389/fgene.2018.00684/fullZ-diskcardiac hypertrophymechanotransductiongene expressionpressure overload |
| spellingShingle | Anastasia Knyazeva Alexander Krutikov Alexey Golovkin Alexander Mishanin Georgii Pavlov Natalia Smolina Natalia Smolina Anastasia Hushkina Thomas Sejersen Gunnar Sjoberg Mikhail Galagudza Anna Kostareva Anna Kostareva Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy Frontiers in Genetics Z-disk cardiac hypertrophy mechanotransduction gene expression pressure overload |
| title | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
| title_full | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
| title_fullStr | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
| title_full_unstemmed | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
| title_short | Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy |
| title_sort | time and ventricular specific expression profiles of genes encoding z disk proteins in pressure overload model of left ventricular hypertrophy |
| topic | Z-disk cardiac hypertrophy mechanotransduction gene expression pressure overload |
| url | https://www.frontiersin.org/article/10.3389/fgene.2018.00684/full |
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