Deoiledjatropha seed cake is a useful nutrient for pullulan production
<p>Abstract</p> <p>Background</p> <p>Ever increasing demand for fossil fuels is a major factor for rapid depletion of these non-renewable energy resources, which has enhanced the interest of finding out alternative sources of energy. In recent years jatropha seed oil ha...
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| Format: | Article |
| Language: | English |
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BMC
2012-03-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://www.microbialcellfactories.com/content/11/1/39 |
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| author | Choudhury Anirban Sharma Nishat Prasad GS |
| author_facet | Choudhury Anirban Sharma Nishat Prasad GS |
| author_sort | Choudhury Anirban |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Ever increasing demand for fossil fuels is a major factor for rapid depletion of these non-renewable energy resources, which has enhanced the interest of finding out alternative sources of energy. In recent years jatropha seed oil has been used extensively for production of bio-diesel and has shown significant potential to replace petroleum fuels at least partially. De-oiled jatropha seed cake (DOJSC) which comprises of approximately 55 to 65% of the biomass is a byproduct of bio-diesel industry. DOJSC contains toxic components like phorbol esters which restricts its utilization as animal feed. Thus along with the enhancement of biodiesel production from jatropha, there is an associated problem of handling this toxic byproduct. Utilization of DOJSC as a feed stock for production of biochemicals may be an attractive solution to the problem.</p> <p>Pullulan is an industrially important polysaccharide with several potential applications in food, pharmaceuticals and cosmetic industries. However, the major bottleneck for commercial utilization of pullulan is its high cost. A cost effective process for pullulan production may be developed using DOJSC as sole nutrient source which will in turn also help in utilization of the byproduct of bio-diesel industry.</p> <p>Results</p> <p>In the present study, DOJSC has been used as a nutrient for production of pullulan, in place of conventional nutrients like yeast extract and peptone. Process optimization was done in shake flasks, and under optimized conditions (8% DOJSC, 15% dextrose, 28°C temperature, 200 rpm, 5% inoculum, 6.0 pH) 83.98 g/L pullulan was obtained. The process was further validated in a 5 L laboratory scale fermenter.</p> <p>Conclusion</p> <p>This is the first report of using DOJSC as nutrient for production of an exopolysaccharide. Successful use of DOJSC as nutrient will help in finding significant application of this toxic byproduct of biodiesel industry. This in turn also have a significant impact on cost reduction and may lead to development of a cost effective green technology for pullulan production.</p> |
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| id | doaj.art-0f6adff2a3864b4c87e522b4d3f0160b |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-13T02:42:47Z |
| publishDate | 2012-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-0f6adff2a3864b4c87e522b4d3f0160b2022-12-22T03:06:09ZengBMCMicrobial Cell Factories1475-28592012-03-011113910.1186/1475-2859-11-39Deoiledjatropha seed cake is a useful nutrient for pullulan productionChoudhury AnirbanSharma NishatPrasad GS<p>Abstract</p> <p>Background</p> <p>Ever increasing demand for fossil fuels is a major factor for rapid depletion of these non-renewable energy resources, which has enhanced the interest of finding out alternative sources of energy. In recent years jatropha seed oil has been used extensively for production of bio-diesel and has shown significant potential to replace petroleum fuels at least partially. De-oiled jatropha seed cake (DOJSC) which comprises of approximately 55 to 65% of the biomass is a byproduct of bio-diesel industry. DOJSC contains toxic components like phorbol esters which restricts its utilization as animal feed. Thus along with the enhancement of biodiesel production from jatropha, there is an associated problem of handling this toxic byproduct. Utilization of DOJSC as a feed stock for production of biochemicals may be an attractive solution to the problem.</p> <p>Pullulan is an industrially important polysaccharide with several potential applications in food, pharmaceuticals and cosmetic industries. However, the major bottleneck for commercial utilization of pullulan is its high cost. A cost effective process for pullulan production may be developed using DOJSC as sole nutrient source which will in turn also help in utilization of the byproduct of bio-diesel industry.</p> <p>Results</p> <p>In the present study, DOJSC has been used as a nutrient for production of pullulan, in place of conventional nutrients like yeast extract and peptone. Process optimization was done in shake flasks, and under optimized conditions (8% DOJSC, 15% dextrose, 28°C temperature, 200 rpm, 5% inoculum, 6.0 pH) 83.98 g/L pullulan was obtained. The process was further validated in a 5 L laboratory scale fermenter.</p> <p>Conclusion</p> <p>This is the first report of using DOJSC as nutrient for production of an exopolysaccharide. Successful use of DOJSC as nutrient will help in finding significant application of this toxic byproduct of biodiesel industry. This in turn also have a significant impact on cost reduction and may lead to development of a cost effective green technology for pullulan production.</p>http://www.microbialcellfactories.com/content/11/1/39JatrophaValue addition to waste<it>Aureobasidium pullulans</it>FermentationExopolysaccharidePullulan |
| spellingShingle | Choudhury Anirban Sharma Nishat Prasad GS Deoiledjatropha seed cake is a useful nutrient for pullulan production Microbial Cell Factories Jatropha Value addition to waste <it>Aureobasidium pullulans</it> Fermentation Exopolysaccharide Pullulan |
| title | Deoiledjatropha seed cake is a useful nutrient for pullulan production |
| title_full | Deoiledjatropha seed cake is a useful nutrient for pullulan production |
| title_fullStr | Deoiledjatropha seed cake is a useful nutrient for pullulan production |
| title_full_unstemmed | Deoiledjatropha seed cake is a useful nutrient for pullulan production |
| title_short | Deoiledjatropha seed cake is a useful nutrient for pullulan production |
| title_sort | deoiledjatropha seed cake is a useful nutrient for pullulan production |
| topic | Jatropha Value addition to waste <it>Aureobasidium pullulans</it> Fermentation Exopolysaccharide Pullulan |
| url | http://www.microbialcellfactories.com/content/11/1/39 |
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