Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle
Aim: The study aimed to identify fatty acid synthase (FASN), LOC514211, and fat mass and obesity-associated (FTO) gene polymorphisms and to investigate their associations with milk traits in an Indonesian-Holstein dairy cow population. Materials and Methods: A total of 100 Indonesian-Holstein cow...
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| Format: | Article |
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Veterinary World
2019-07-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.12/July-2019/35.pdf |
| _version_ | 1828952496860561408 |
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| author | Amalia Puji Rahayu Tety Hartatik Agung Purnomoadi Edy Kurnianto |
| author_facet | Amalia Puji Rahayu Tety Hartatik Agung Purnomoadi Edy Kurnianto |
| author_sort | Amalia Puji Rahayu |
| collection | DOAJ |
| description | Aim: The study aimed to identify fatty acid synthase (FASN), LOC514211, and fat mass and obesity-associated (FTO) gene polymorphisms and to investigate their associations with milk traits in an Indonesian-Holstein dairy cow population.
Materials and Methods: A total of 100 Indonesian-Holstein cows consisting of 50 heads (0th generation; G0) and 50 heads of their daughters (1st generation; G1) were used. Polymerase chain reaction-restriction fragment length polymorphism was performed to genotype three single nucleotide polymorphisms: rs41919985 in the FASN gene, rs42688595 in the LOC514211 gene, and g.1371T>A in the FTO gene.
Results: FASN rs41919985 was associated with milk protein percentage (p<0.05), FTO g.1371T>A was associated with milk fat percentage (p<0.05), and LOC514211 rs42688595 was not associated with any trait (p>0.05). Heterozygote variants showed a higher protein percentage for FASN and the highest fat percentage for FTO. These associations were consistent in the G0 and G1 populations.
Conclusion: Our results indicate that the milk protein and fat percentages can be improved by increasing the frequency of the AG genotype of FASN and the AT genotype of FTO, respectively. |
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| format | Article |
| id | doaj.art-0f70df4cb0064c1a9e46dff29a8045b0 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-14T06:53:30Z |
| publishDate | 2019-07-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-0f70df4cb0064c1a9e46dff29a8045b02022-12-21T23:12:48ZengVeterinary WorldVeterinary World0972-89882231-09162019-07-011271160116610.14202/vetworld.2019.1160-1166Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattleAmalia Puji Rahayu0Tety Hartatik1Agung Purnomoadi2Edy Kurnianto3Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, Indonesia; Agriculture, Fisheries and Food Center of Semarang Regency, Ungaran 50514, Indonesia.Department of Animal Breeding and Reproduction, Faculty of Animal Sciences, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, Indonesia.Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, Indonesia.Aim: The study aimed to identify fatty acid synthase (FASN), LOC514211, and fat mass and obesity-associated (FTO) gene polymorphisms and to investigate their associations with milk traits in an Indonesian-Holstein dairy cow population. Materials and Methods: A total of 100 Indonesian-Holstein cows consisting of 50 heads (0th generation; G0) and 50 heads of their daughters (1st generation; G1) were used. Polymerase chain reaction-restriction fragment length polymorphism was performed to genotype three single nucleotide polymorphisms: rs41919985 in the FASN gene, rs42688595 in the LOC514211 gene, and g.1371T>A in the FTO gene. Results: FASN rs41919985 was associated with milk protein percentage (p<0.05), FTO g.1371T>A was associated with milk fat percentage (p<0.05), and LOC514211 rs42688595 was not associated with any trait (p>0.05). Heterozygote variants showed a higher protein percentage for FASN and the highest fat percentage for FTO. These associations were consistent in the G0 and G1 populations. Conclusion: Our results indicate that the milk protein and fat percentages can be improved by increasing the frequency of the AG genotype of FASN and the AT genotype of FTO, respectively.http://www.veterinaryworld.org/Vol.12/July-2019/35.pdffat mass and obesity-associatedfatty acid synthaseIndonesian-Holstein cattleLOC514211milk traits |
| spellingShingle | Amalia Puji Rahayu Tety Hartatik Agung Purnomoadi Edy Kurnianto Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle Veterinary World fat mass and obesity-associated fatty acid synthase Indonesian-Holstein cattle LOC514211 milk traits |
| title | Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle |
| title_full | Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle |
| title_fullStr | Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle |
| title_full_unstemmed | Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle |
| title_short | Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle |
| title_sort | association of single nucleotide polymorphisms in the fatty acid synthase loc514211 and fat mass and obesity associated genes with milk traits in indonesian holstein dairy cattle |
| topic | fat mass and obesity-associated fatty acid synthase Indonesian-Holstein cattle LOC514211 milk traits |
| url | http://www.veterinaryworld.org/Vol.12/July-2019/35.pdf |
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