PSMB2 plays an oncogenic role in glioma and correlates to the immune microenvironment
Abstract There has been an upward trend in the incidence of glioma, with high recurrence and high mortality. The beta subunits of the 20S proteasome are encoded by the proteasome beta (PSMB) genes and may affect the proteasome’s function in glioma, assembly and inhibitor binding. This study attempte...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-03-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-56493-5 |
| _version_ | 1827315950777532416 |
|---|---|
| author | Wei He Zhe Zhang ZiLong Tan XinXian Liu ZeKun Wang Bo Xiong XiaoLi Shen XinGen Zhu |
| author_facet | Wei He Zhe Zhang ZiLong Tan XinXian Liu ZeKun Wang Bo Xiong XiaoLi Shen XinGen Zhu |
| author_sort | Wei He |
| collection | DOAJ |
| description | Abstract There has been an upward trend in the incidence of glioma, with high recurrence and high mortality. The beta subunits of the 20S proteasome are encoded by the proteasome beta (PSMB) genes and may affect the proteasome’s function in glioma, assembly and inhibitor binding. This study attempted to reveal the function of the proliferation and invasion of glioma cells, which is affected by proteasome 20S subunit beta 2 (PSMB2). We subjected the data downloaded from the TCGA database to ROC, survival, and enrichment analyses. After establishing the stable PSMB2 knockdown glioma cell line. We detect the changes in the proliferation, invasion and migration of glioma cells by plate colony formation assay, transwell assay, wound healing assay and flow cytometry and PSMB2 expression was verified by quantitative PCR and Western blotting to identify the mRNA and protein levels. PSMB2 expression was higher in glioma tissues, and its expression positively correlated with poor prognosis and high tumor grade and after PSMB2 knockdown, the proliferation, invasion and migration of glioma cells were weakened. |
| first_indexed | 2024-04-24T23:06:39Z |
| format | Article |
| id | doaj.art-0f70f12e191e4eaaa80c7b0302856be5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T23:06:39Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0f70f12e191e4eaaa80c7b0302856be52024-03-17T12:26:29ZengNature PortfolioScientific Reports2045-23222024-03-0114111210.1038/s41598-024-56493-5PSMB2 plays an oncogenic role in glioma and correlates to the immune microenvironmentWei He0Zhe Zhang1ZiLong Tan2XinXian Liu3ZeKun Wang4Bo Xiong5XiaoLi Shen6XinGen Zhu7Department of Neurosurgery, The Second affiliated hospital, Jiangxi Medical College, Nanchang UniversityDepartment of Neurosurgery, The Second affiliated hospital, Jiangxi Medical College, Nanchang UniversityDepartment of Neurosurgery, The Second affiliated hospital, Jiangxi Medical College, Nanchang UniversityDepartment of Neurosurgery, The Second affiliated hospital, Jiangxi Medical College, Nanchang UniversityDepartment of Neurosurgery, The Second affiliated hospital, Jiangxi Medical College, Nanchang UniversityDepartment of Neurosurgery, The People’s Hospital of Gao anDepartment of Neurosurgery, The Second affiliated hospital, Jiangxi Medical College, Nanchang UniversityDepartment of Neurosurgery, The Second affiliated hospital, Jiangxi Medical College, Nanchang UniversityAbstract There has been an upward trend in the incidence of glioma, with high recurrence and high mortality. The beta subunits of the 20S proteasome are encoded by the proteasome beta (PSMB) genes and may affect the proteasome’s function in glioma, assembly and inhibitor binding. This study attempted to reveal the function of the proliferation and invasion of glioma cells, which is affected by proteasome 20S subunit beta 2 (PSMB2). We subjected the data downloaded from the TCGA database to ROC, survival, and enrichment analyses. After establishing the stable PSMB2 knockdown glioma cell line. We detect the changes in the proliferation, invasion and migration of glioma cells by plate colony formation assay, transwell assay, wound healing assay and flow cytometry and PSMB2 expression was verified by quantitative PCR and Western blotting to identify the mRNA and protein levels. PSMB2 expression was higher in glioma tissues, and its expression positively correlated with poor prognosis and high tumor grade and after PSMB2 knockdown, the proliferation, invasion and migration of glioma cells were weakened.https://doi.org/10.1038/s41598-024-56493-5GliomaPSMB2ProliferationInvasion |
| spellingShingle | Wei He Zhe Zhang ZiLong Tan XinXian Liu ZeKun Wang Bo Xiong XiaoLi Shen XinGen Zhu PSMB2 plays an oncogenic role in glioma and correlates to the immune microenvironment Scientific Reports Glioma PSMB2 Proliferation Invasion |
| title | PSMB2 plays an oncogenic role in glioma and correlates to the immune microenvironment |
| title_full | PSMB2 plays an oncogenic role in glioma and correlates to the immune microenvironment |
| title_fullStr | PSMB2 plays an oncogenic role in glioma and correlates to the immune microenvironment |
| title_full_unstemmed | PSMB2 plays an oncogenic role in glioma and correlates to the immune microenvironment |
| title_short | PSMB2 plays an oncogenic role in glioma and correlates to the immune microenvironment |
| title_sort | psmb2 plays an oncogenic role in glioma and correlates to the immune microenvironment |
| topic | Glioma PSMB2 Proliferation Invasion |
| url | https://doi.org/10.1038/s41598-024-56493-5 |
| work_keys_str_mv | AT weihe psmb2playsanoncogenicroleingliomaandcorrelatestotheimmunemicroenvironment AT zhezhang psmb2playsanoncogenicroleingliomaandcorrelatestotheimmunemicroenvironment AT zilongtan psmb2playsanoncogenicroleingliomaandcorrelatestotheimmunemicroenvironment AT xinxianliu psmb2playsanoncogenicroleingliomaandcorrelatestotheimmunemicroenvironment AT zekunwang psmb2playsanoncogenicroleingliomaandcorrelatestotheimmunemicroenvironment AT boxiong psmb2playsanoncogenicroleingliomaandcorrelatestotheimmunemicroenvironment AT xiaolishen psmb2playsanoncogenicroleingliomaandcorrelatestotheimmunemicroenvironment AT xingenzhu psmb2playsanoncogenicroleingliomaandcorrelatestotheimmunemicroenvironment |