Multivariate modelling with <sup>1</sup>H NMR of pleural effusion in murine cerebral malaria
<p>Abstract</p> <p>Background</p> <p>Cerebral malaria is a clinical manifestation of <it>Plasmodium falciparum </it>infection. Although brain damage is the predominant pathophysiological complication of cerebral malaria (CM), respiratory distress, acute lung...
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BMC
2011-11-01
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| Series: | Malaria Journal |
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| Online Access: | http://www.malariajournal.com/content/10/1/330 |
| _version_ | 1818163659817353216 |
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| author | Ghosh Soumita Sengupta Arjun Sharma Shobhona Sonawat Haripalsingh M |
| author_facet | Ghosh Soumita Sengupta Arjun Sharma Shobhona Sonawat Haripalsingh M |
| author_sort | Ghosh Soumita |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cerebral malaria is a clinical manifestation of <it>Plasmodium falciparum </it>infection. Although brain damage is the predominant pathophysiological complication of cerebral malaria (CM), respiratory distress, acute lung injury, hydrothorax/pleural effusion are also observed in several cases. Immunological parameters have been assessed in pleural fluid in murine models; however there are no reports of characterization of metabolites present in pleural effusion.</p> <p>Methods</p> <p><sup>1</sup>H NMR of the sera and the pleural effusion of cerebral malaria infected mice were analyzed using principal component analysis, orthogonal partial least square analysis, multiway principal component analysis, and multivariate curve resolution.</p> <p>Results</p> <p>It has been observed that there was 100% occurrence of pleural effusion (PE) in the mice affected with CM, as opposed to those are non-cerebral and succumbing to hyperparasitaemia (NCM/HP). An analysis of <sup>1</sup>H NMR and SDS-PAGE profile of PE and serum samples of each of the CM mice exhibited a similar profile in terms of constituents. Multivariate analysis on these two classes of biofluids was performed and significant differences were detected in concentrations of metabolites. Glucose, creatine and glutamine contents were high in the PE and lipids being high in the sera. Multivariate curve resolution between sera and pleural effusion showed that changes in PE co-varied with that of serum in CM mice. The increase of glucose in PE is negatively correlated to the glucose in serum in CM as obtained from the result of multiway principal component analysis.</p> <p>Conclusions</p> <p>This study reports for the first time, the characterization of metabolites in pleural effusion formed during murine cerebral malaria. The study indicates that the origin of PE metabolites in murine CM may be the serum. The loss of the components like glucose, glutamine and creatine into the PE may worsen the situation of patients, in conjunction with the enhanced glycolysis, glutaminolysis and increased activity of creatine phophokinase which are already reported characteristic pathophysiological features of malaria.</p> |
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| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-11T16:53:05Z |
| publishDate | 2011-11-01 |
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| series | Malaria Journal |
| spelling | doaj.art-0f71c97c027d4110a460511550c717822022-12-22T00:58:03ZengBMCMalaria Journal1475-28752011-11-0110133010.1186/1475-2875-10-330Multivariate modelling with <sup>1</sup>H NMR of pleural effusion in murine cerebral malariaGhosh SoumitaSengupta ArjunSharma ShobhonaSonawat Haripalsingh M<p>Abstract</p> <p>Background</p> <p>Cerebral malaria is a clinical manifestation of <it>Plasmodium falciparum </it>infection. Although brain damage is the predominant pathophysiological complication of cerebral malaria (CM), respiratory distress, acute lung injury, hydrothorax/pleural effusion are also observed in several cases. Immunological parameters have been assessed in pleural fluid in murine models; however there are no reports of characterization of metabolites present in pleural effusion.</p> <p>Methods</p> <p><sup>1</sup>H NMR of the sera and the pleural effusion of cerebral malaria infected mice were analyzed using principal component analysis, orthogonal partial least square analysis, multiway principal component analysis, and multivariate curve resolution.</p> <p>Results</p> <p>It has been observed that there was 100% occurrence of pleural effusion (PE) in the mice affected with CM, as opposed to those are non-cerebral and succumbing to hyperparasitaemia (NCM/HP). An analysis of <sup>1</sup>H NMR and SDS-PAGE profile of PE and serum samples of each of the CM mice exhibited a similar profile in terms of constituents. Multivariate analysis on these two classes of biofluids was performed and significant differences were detected in concentrations of metabolites. Glucose, creatine and glutamine contents were high in the PE and lipids being high in the sera. Multivariate curve resolution between sera and pleural effusion showed that changes in PE co-varied with that of serum in CM mice. The increase of glucose in PE is negatively correlated to the glucose in serum in CM as obtained from the result of multiway principal component analysis.</p> <p>Conclusions</p> <p>This study reports for the first time, the characterization of metabolites in pleural effusion formed during murine cerebral malaria. The study indicates that the origin of PE metabolites in murine CM may be the serum. The loss of the components like glucose, glutamine and creatine into the PE may worsen the situation of patients, in conjunction with the enhanced glycolysis, glutaminolysis and increased activity of creatine phophokinase which are already reported characteristic pathophysiological features of malaria.</p>http://www.malariajournal.com/content/10/1/330Cerebral Malaria<it>Plasmodium berghei </it>ANKApleural effusionNMROrthogonal Partial Least Square Discriminant AnalysisMultiway Principal Component AnalysisMultivariate Curve Resolution |
| spellingShingle | Ghosh Soumita Sengupta Arjun Sharma Shobhona Sonawat Haripalsingh M Multivariate modelling with <sup>1</sup>H NMR of pleural effusion in murine cerebral malaria Malaria Journal Cerebral Malaria <it>Plasmodium berghei </it>ANKA pleural effusion NMR Orthogonal Partial Least Square Discriminant Analysis Multiway Principal Component Analysis Multivariate Curve Resolution |
| title | Multivariate modelling with <sup>1</sup>H NMR of pleural effusion in murine cerebral malaria |
| title_full | Multivariate modelling with <sup>1</sup>H NMR of pleural effusion in murine cerebral malaria |
| title_fullStr | Multivariate modelling with <sup>1</sup>H NMR of pleural effusion in murine cerebral malaria |
| title_full_unstemmed | Multivariate modelling with <sup>1</sup>H NMR of pleural effusion in murine cerebral malaria |
| title_short | Multivariate modelling with <sup>1</sup>H NMR of pleural effusion in murine cerebral malaria |
| title_sort | multivariate modelling with sup 1 sup h nmr of pleural effusion in murine cerebral malaria |
| topic | Cerebral Malaria <it>Plasmodium berghei </it>ANKA pleural effusion NMR Orthogonal Partial Least Square Discriminant Analysis Multiway Principal Component Analysis Multivariate Curve Resolution |
| url | http://www.malariajournal.com/content/10/1/330 |
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