An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas
We studied the acute toxicity of an imazamox-based herbicide at 12, 24 and 36 mg/kg body (bw) weight imazamox equivalent dose on the liver and pancreatic tissue in Sprague Dawley rats. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, glucose, calcium as well as creatin...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Toxicology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750018306875 |
| _version_ | 1818335395383869440 |
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| author | Çiğdem Sevim Selim Çomaklı Ali Taghizadehghalehjoughi Mustafa Özkaraca Robin Mesnage Leda Kovatsi Tatyana I. Burykina Alexandra Kalogeraki Michael N. Antoniou Aristidis Tsatsakis |
| author_facet | Çiğdem Sevim Selim Çomaklı Ali Taghizadehghalehjoughi Mustafa Özkaraca Robin Mesnage Leda Kovatsi Tatyana I. Burykina Alexandra Kalogeraki Michael N. Antoniou Aristidis Tsatsakis |
| author_sort | Çiğdem Sevim |
| collection | DOAJ |
| description | We studied the acute toxicity of an imazamox-based herbicide at 12, 24 and 36 mg/kg body (bw) weight imazamox equivalent dose on the liver and pancreatic tissue in Sprague Dawley rats. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, glucose, calcium as well as creatinine, were determined in blood samples, which were collected after 24, 48 and 72 h exposure. Caspase 3 and anti-insulin expression and immunopositivity were evaluated using in situ hybridization and immunohistochemistry, respectively. The imazamox-based herbicide evaluated in this study induced toxic effects even from the lowest dose tested (12 mg/kg bw). The two highest doses caused a statistically significant cytotoxicity on the Langerhans islet cells. Necrotic and degenerative changes were detected in hepatocytes at the two highest doses. Imazamox is considered to be poorly toxic to the liver. Nevertheless, the imazamox-based herbicide formulation tested here reduced the size of the β-islet cells, induced an elevation in serum glucose and calcium. Our data shows that commercial formulations of imazamox containing various co-formulants can have hepatic and pancreatic toxic effects. Keywords: Anti-insulin, Caspase 3, Imazamox, Immunohistochemistry, In situ hybridization |
| first_indexed | 2024-12-13T14:22:45Z |
| format | Article |
| id | doaj.art-0f72c0a29bd5437f9a2af82c96c3d543 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-13T14:22:45Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-0f72c0a29bd5437f9a2af82c96c3d5432022-12-21T23:42:02ZengElsevierToxicology Reports2214-75002019-01-0164250An imazamox-based herbicide causes apoptotic changes in rat liver and pancreasÇiğdem Sevim0Selim Çomaklı1Ali Taghizadehghalehjoughi2Mustafa Özkaraca3Robin Mesnage4Leda Kovatsi5Tatyana I. Burykina6Alexandra Kalogeraki7Michael N. Antoniou8Aristidis Tsatsakis9Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey; Correspondence author.Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, TurkeyDepartment of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, TurkeyDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, TurkeyDepartment of Medical and Molecular Genetics, King's College London, London, United KingdomLaboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, GreeceDepartment of Analytical and Forensic Medical Toxicology, Sechenov University, 2-4 Bolshaya Pirogovskaya st., 119991 Moscow, RussiaDepartment of Pathology–Cytopathology, Medical School, University of Crete, Heraklion, GreeceDepartment of Medical and Molecular Genetics, King's College London, London, United KingdomDepartment of Toxicology & Forensic Sciences, Faculty Medicine, University of Crete, Heraklion, GreeceWe studied the acute toxicity of an imazamox-based herbicide at 12, 24 and 36 mg/kg body (bw) weight imazamox equivalent dose on the liver and pancreatic tissue in Sprague Dawley rats. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, glucose, calcium as well as creatinine, were determined in blood samples, which were collected after 24, 48 and 72 h exposure. Caspase 3 and anti-insulin expression and immunopositivity were evaluated using in situ hybridization and immunohistochemistry, respectively. The imazamox-based herbicide evaluated in this study induced toxic effects even from the lowest dose tested (12 mg/kg bw). The two highest doses caused a statistically significant cytotoxicity on the Langerhans islet cells. Necrotic and degenerative changes were detected in hepatocytes at the two highest doses. Imazamox is considered to be poorly toxic to the liver. Nevertheless, the imazamox-based herbicide formulation tested here reduced the size of the β-islet cells, induced an elevation in serum glucose and calcium. Our data shows that commercial formulations of imazamox containing various co-formulants can have hepatic and pancreatic toxic effects. Keywords: Anti-insulin, Caspase 3, Imazamox, Immunohistochemistry, In situ hybridizationhttp://www.sciencedirect.com/science/article/pii/S2214750018306875 |
| spellingShingle | Çiğdem Sevim Selim Çomaklı Ali Taghizadehghalehjoughi Mustafa Özkaraca Robin Mesnage Leda Kovatsi Tatyana I. Burykina Alexandra Kalogeraki Michael N. Antoniou Aristidis Tsatsakis An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas Toxicology Reports |
| title | An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas |
| title_full | An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas |
| title_fullStr | An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas |
| title_full_unstemmed | An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas |
| title_short | An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas |
| title_sort | imazamox based herbicide causes apoptotic changes in rat liver and pancreas |
| url | http://www.sciencedirect.com/science/article/pii/S2214750018306875 |
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