Development of a Seed Treatment with <i>Pochonia chlamydosporia</i> for Biocontrol Application
Seed treatment is a powerful technique for adding beneficial ingredients to plants during the seed preparation process. Biopolymers as drying agents and delivery systems in seed treatments were investigated for their biocompatibility with blastospores of the nematophagous fungus <i>Pochonia ch...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/14/1/138 |
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| author | Jana Uthoff Desiree Jakobs-Schönwandt Karl-Josef Dietz Anant Patel |
| author_facet | Jana Uthoff Desiree Jakobs-Schönwandt Karl-Josef Dietz Anant Patel |
| author_sort | Jana Uthoff |
| collection | DOAJ |
| description | Seed treatment is a powerful technique for adding beneficial ingredients to plants during the seed preparation process. Biopolymers as drying agents and delivery systems in seed treatments were investigated for their biocompatibility with blastospores of the nematophagous fungus <i>Pochonia chlamydosporia</i>. To produce a novel seed treatment for the cover crop <i>Phacelia tanacetifolia</i>, xanthan gum TG and gellan gum were the most promising biopolymers in combination with potato starch and bentonite. The seed treatment process as well as the drying process were specially designed to be scalable, which make it suitable for applying the developed seed treatment in agriculture. Application of gellan gum in seed treatments led to 6.3% ± 1.6% of vital blastospores per seed compared to 3.8% ± 0.3% of vital blastospores when applying xanthan gum. Storage tests for seed treatments with 0.5% gellan gum indicated a higher stability at 4 °C compared to storage at 21 °C. After 42 days of storage at 4 °C, 54.1% ± 15.1% of the applied blastospores were viable compared to 0.3% ± 0.8% at 21 °C. This novel seed treatment application with <i>P. chlamydosporia</i> blastospores includes the seed treatment procedure, drying process, and storage tests and can easily be upscaled for application in agriculture. |
| first_indexed | 2024-03-08T09:59:31Z |
| format | Article |
| id | doaj.art-0f7366280af34fab8961ead0faf3071e |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-08T09:59:31Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-0f7366280af34fab8961ead0faf3071e2024-01-29T13:40:43ZengMDPI AGAgriculture2077-04722024-01-0114113810.3390/agriculture14010138Development of a Seed Treatment with <i>Pochonia chlamydosporia</i> for Biocontrol ApplicationJana Uthoff0Desiree Jakobs-Schönwandt1Karl-Josef Dietz2Anant Patel3Fermentation and Formulation of Biologicals and Chemicals, Hochschule Bielefeld-University of Applied Sciences and Arts (HSBI), Interaktion 1, 33619 Bielefeld, GermanyFermentation and Formulation of Biologicals and Chemicals, Hochschule Bielefeld-University of Applied Sciences and Arts (HSBI), Interaktion 1, 33619 Bielefeld, GermanyPlant Biochemistry and Physiology, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, GermanyFermentation and Formulation of Biologicals and Chemicals, Hochschule Bielefeld-University of Applied Sciences and Arts (HSBI), Interaktion 1, 33619 Bielefeld, GermanySeed treatment is a powerful technique for adding beneficial ingredients to plants during the seed preparation process. Biopolymers as drying agents and delivery systems in seed treatments were investigated for their biocompatibility with blastospores of the nematophagous fungus <i>Pochonia chlamydosporia</i>. To produce a novel seed treatment for the cover crop <i>Phacelia tanacetifolia</i>, xanthan gum TG and gellan gum were the most promising biopolymers in combination with potato starch and bentonite. The seed treatment process as well as the drying process were specially designed to be scalable, which make it suitable for applying the developed seed treatment in agriculture. Application of gellan gum in seed treatments led to 6.3% ± 1.6% of vital blastospores per seed compared to 3.8% ± 0.3% of vital blastospores when applying xanthan gum. Storage tests for seed treatments with 0.5% gellan gum indicated a higher stability at 4 °C compared to storage at 21 °C. After 42 days of storage at 4 °C, 54.1% ± 15.1% of the applied blastospores were viable compared to 0.3% ± 0.8% at 21 °C. This novel seed treatment application with <i>P. chlamydosporia</i> blastospores includes the seed treatment procedure, drying process, and storage tests and can easily be upscaled for application in agriculture.https://www.mdpi.com/2077-0472/14/1/138seed treatment<i>Pochonia chlamydosporia</i>biopolymersshelf life |
| spellingShingle | Jana Uthoff Desiree Jakobs-Schönwandt Karl-Josef Dietz Anant Patel Development of a Seed Treatment with <i>Pochonia chlamydosporia</i> for Biocontrol Application Agriculture seed treatment <i>Pochonia chlamydosporia</i> biopolymers shelf life |
| title | Development of a Seed Treatment with <i>Pochonia chlamydosporia</i> for Biocontrol Application |
| title_full | Development of a Seed Treatment with <i>Pochonia chlamydosporia</i> for Biocontrol Application |
| title_fullStr | Development of a Seed Treatment with <i>Pochonia chlamydosporia</i> for Biocontrol Application |
| title_full_unstemmed | Development of a Seed Treatment with <i>Pochonia chlamydosporia</i> for Biocontrol Application |
| title_short | Development of a Seed Treatment with <i>Pochonia chlamydosporia</i> for Biocontrol Application |
| title_sort | development of a seed treatment with i pochonia chlamydosporia i for biocontrol application |
| topic | seed treatment <i>Pochonia chlamydosporia</i> biopolymers shelf life |
| url | https://www.mdpi.com/2077-0472/14/1/138 |
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