In silico and experimental approaches for validating RNA editing events in transcriptomes
As a typical RNA virus, SARS-CoV-2 is subjected to RNA editing in host cells. While some researchers believe that a traditional variant calling pipeline retrieves all true-positive RNA editing events from the transcriptome, others argue that conventional methods identify many false-positive sites. H...
| मुख्य लेखक: | |
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| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
Taylor & Francis Group
2024-12-01
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| श्रृंखला: | RNA Biology |
| विषय: | |
| ऑनलाइन पहुंच: | https://www.tandfonline.com/doi/10.1080/15476286.2024.2432729 |
| सारांश: | As a typical RNA virus, SARS-CoV-2 is subjected to RNA editing in host cells. While some researchers believe that a traditional variant calling pipeline retrieves all true-positive RNA editing events from the transcriptome, others argue that conventional methods identify many false-positive sites. Here, I describe several additional in silico and experimental approaches to validate the authenticity of RNA editing in SARS-CoV-2. These approaches include requiring strand-specific sequencing, analysis of hyperedited reads, linkage analysis, orthogonal methods like mass spectrometry, and the use of ADAR-deficient host cells. These findings may improve future analyses on the identification of RNA editing, especially in RNA viruses. |
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| आईएसएसएन: | 1547-6286 1555-8584 |