Results with the establishment of in vitro culture of Leucojum aestivum
Leucojum aestivum is a native, protected ornamental and medicinal plant in Hungary and in Ukraine too. The aim of our work was to establish in vitro cultures of this bulbous plant. Prior to surface sterilisation the old leaves and roots were dissected from the bulbs and they were stored in a refrige...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Debrecen
2007-05-01
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| Series: | International Journal of Horticultural Science |
| Subjects: | |
| Online Access: | https://ojs.lib.unideb.hu/IJHS/article/view/723 |
| _version_ | 1818146008689803264 |
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| author | E. Kohut M. Ördögh E. Jámbor-Benczúr Á. Máthé |
| author_facet | E. Kohut M. Ördögh E. Jámbor-Benczúr Á. Máthé |
| author_sort | E. Kohut |
| collection | DOAJ |
| description | Leucojum aestivum is a native, protected ornamental and medicinal plant in Hungary and in Ukraine too. The aim of our work was to establish in vitro cultures of this bulbous plant. Prior to surface sterilisation the old leaves and roots were dissected from the bulbs and they were stored in a refrigerator (2-3°C) for different periods (1 week for the first starting experiment and 5 weeks for the second one). After sterilisation, bulbs, bulb scales and leaves of the bulbs were placed on Murashige and Skoog's (1962) medium with 1 mg/1 benzyl-adenine (BA) and 0,1 mg/1 naphthalene acetic acid (NAA). At the first starting experiment 81,3%, and at the second one 92,3% of the explants turned to be sterile. Bulblets and roots were developed on the explants in the case of using bulb plates together with bulb scales and leaves as inoculua. The best result was achieved after 5 weeks chilling and it was possible to gain little bulbs from the bulb leaves too. |
| first_indexed | 2024-12-11T12:12:31Z |
| format | Article |
| id | doaj.art-0f74efc41674490c8e64f8c142a827fb |
| institution | Directory Open Access Journal |
| issn | 1585-0404 2676-931X |
| language | English |
| last_indexed | 2024-12-11T12:12:31Z |
| publishDate | 2007-05-01 |
| publisher | University of Debrecen |
| record_format | Article |
| series | International Journal of Horticultural Science |
| spelling | doaj.art-0f74efc41674490c8e64f8c142a827fb2022-12-22T01:07:45ZengUniversity of DebrecenInternational Journal of Horticultural Science1585-04042676-931X2007-05-0113210.31421/IJHS/13/2/723Results with the establishment of in vitro culture of Leucojum aestivumE. Kohut0M. Ördögh1E. Jámbor-Benczúr2Á. Máthé3Corvinus University of Budapest, Faculty of Horticultural Science, Department of Floriculture and DendrologyCorvinus University of Budapest, Faculty of Horticultural Science, Department of Floriculture and DendrologyCorvinus University of Budapest, Faculty of Horticultural Science, Department of Floriculture and DendrologyWest-Hungarian University, Faculty of Agriculture and Food Science, Department of BotanyLeucojum aestivum is a native, protected ornamental and medicinal plant in Hungary and in Ukraine too. The aim of our work was to establish in vitro cultures of this bulbous plant. Prior to surface sterilisation the old leaves and roots were dissected from the bulbs and they were stored in a refrigerator (2-3°C) for different periods (1 week for the first starting experiment and 5 weeks for the second one). After sterilisation, bulbs, bulb scales and leaves of the bulbs were placed on Murashige and Skoog's (1962) medium with 1 mg/1 benzyl-adenine (BA) and 0,1 mg/1 naphthalene acetic acid (NAA). At the first starting experiment 81,3%, and at the second one 92,3% of the explants turned to be sterile. Bulblets and roots were developed on the explants in the case of using bulb plates together with bulb scales and leaves as inoculua. The best result was achieved after 5 weeks chilling and it was possible to gain little bulbs from the bulb leaves too.https://ojs.lib.unideb.hu/IJHS/article/view/723Leucojum aestivumin vitro culturesterilisationchilling |
| spellingShingle | E. Kohut M. Ördögh E. Jámbor-Benczúr Á. Máthé Results with the establishment of in vitro culture of Leucojum aestivum International Journal of Horticultural Science Leucojum aestivum in vitro culture sterilisation chilling |
| title | Results with the establishment of in vitro culture of Leucojum aestivum |
| title_full | Results with the establishment of in vitro culture of Leucojum aestivum |
| title_fullStr | Results with the establishment of in vitro culture of Leucojum aestivum |
| title_full_unstemmed | Results with the establishment of in vitro culture of Leucojum aestivum |
| title_short | Results with the establishment of in vitro culture of Leucojum aestivum |
| title_sort | results with the establishment of in vitro culture of leucojum aestivum |
| topic | Leucojum aestivum in vitro culture sterilisation chilling |
| url | https://ojs.lib.unideb.hu/IJHS/article/view/723 |
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