Salamander retina phospholipids and their localization by MALDI imaging mass spectrometry at cellular size resolution[S]
Salamander large cells facilitated identification and localization of lipids by MALDI imaging mass spectrometry. Salamander retina lipid extract showed similarity with rodent retina lipid extract in phospholipid content and composition. Like rodent retina section, distinct layer distributions of pho...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2011-03-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520409265 |
| _version_ | 1819077214944100352 |
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| author | Michael C. Roy Hiroki Nakanishi Kazuteru Takahashi Setsuko Nakanishi Shigeki Kajihara Takahiro Hayasaka Mitsutoshi Setou Kiyoshi Ogawa Ryo Taguchi Takayuki Naito |
| author_facet | Michael C. Roy Hiroki Nakanishi Kazuteru Takahashi Setsuko Nakanishi Shigeki Kajihara Takahiro Hayasaka Mitsutoshi Setou Kiyoshi Ogawa Ryo Taguchi Takayuki Naito |
| author_sort | Michael C. Roy |
| collection | DOAJ |
| description | Salamander large cells facilitated identification and localization of lipids by MALDI imaging mass spectrometry. Salamander retina lipid extract showed similarity with rodent retina lipid extract in phospholipid content and composition. Like rodent retina section, distinct layer distributions of phospholipids were observed in the salamander retina section. Phosphatidylcholines (PCs) composing saturated and monounsaturated fatty acids (PC 32:0, PC 32:1, and PC 34:1) were detected mainly in the outer and inner plexiform layers (OPL and IPL), whereas PCs containing polyunsaturated fatty acids (PC 36:4, PC 38:6, and PC 40:6) composed the inner segment (IS) and outer segment (OS). The presence of PCs containing polyunsaturated fatty acids in the OS layer implied that these phospholipids form flexible lipid bilayers, which facilitate phototransduction process occurring in the rhodopsin rich OS layer. Distinct distributions and relative signal intensities of phospholipids also indicated their relative abundance in a particular cell or a cell part. Using salamander large cells, a single cell level localization and identification of biomolecules could be achieved by MALDI imaging mass spectrometry. |
| first_indexed | 2024-12-21T18:53:39Z |
| format | Article |
| id | doaj.art-0f7b26aa3039448e8de3977fbe76bc0a |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T18:53:39Z |
| publishDate | 2011-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-0f7b26aa3039448e8de3977fbe76bc0a2022-12-21T18:53:41ZengElsevierJournal of Lipid Research0022-22752011-03-01523463470Salamander retina phospholipids and their localization by MALDI imaging mass spectrometry at cellular size resolution[S]Michael C. Roy0Hiroki Nakanishi1Kazuteru Takahashi2Setsuko Nakanishi3Shigeki Kajihara4Takahiro Hayasaka5Mitsutoshi Setou6Kiyoshi Ogawa7Ryo Taguchi8Takayuki Naito9To whom correspondence should be addressed. mcroy@oist.jp; Molecular Neuroscience Unit, Okinawa Institute of Science and Technology (OIST), 12-22 Suzaki, Uruma, Okinawa 904-2234, Japan; Department of Metabolome, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, JapanCore Research for Evolutional Science and Technology, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, JapanTechnology Research Laboratory, Shimadzu Corporation, 3-9-4 Soraku-gun, Seika-cho, Kyoto 619-0237, JapanMolecular Neuroscience Unit, Okinawa Institute of Science and Technology (OIST), 12-22 Suzaki, Uruma, Okinawa 904-2234, JapanTechnology Research Laboratory, Shimadzu Corporation, 3-9-4 Soraku-gun, Seika-cho, Kyoto 619-0237, JapanDepartment of Molecular Anatomy, Molecular Imaging Frontier Research Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Molecular Anatomy, Molecular Imaging Frontier Research Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanTechnology Research Laboratory, Shimadzu Corporation, 3-9-4 Soraku-gun, Seika-cho, Kyoto 619-0237, JapanCore Research for Evolutional Science and Technology, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, JapanMolecular Neuroscience Unit, Okinawa Institute of Science and Technology (OIST), 12-22 Suzaki, Uruma, Okinawa 904-2234, JapanSalamander large cells facilitated identification and localization of lipids by MALDI imaging mass spectrometry. Salamander retina lipid extract showed similarity with rodent retina lipid extract in phospholipid content and composition. Like rodent retina section, distinct layer distributions of phospholipids were observed in the salamander retina section. Phosphatidylcholines (PCs) composing saturated and monounsaturated fatty acids (PC 32:0, PC 32:1, and PC 34:1) were detected mainly in the outer and inner plexiform layers (OPL and IPL), whereas PCs containing polyunsaturated fatty acids (PC 36:4, PC 38:6, and PC 40:6) composed the inner segment (IS) and outer segment (OS). The presence of PCs containing polyunsaturated fatty acids in the OS layer implied that these phospholipids form flexible lipid bilayers, which facilitate phototransduction process occurring in the rhodopsin rich OS layer. Distinct distributions and relative signal intensities of phospholipids also indicated their relative abundance in a particular cell or a cell part. Using salamander large cells, a single cell level localization and identification of biomolecules could be achieved by MALDI imaging mass spectrometry.http://www.sciencedirect.com/science/article/pii/S0022227520409265matrix-assisted laser desorption/ionizationAmbystoma mexicanumlipidomicsphospholipid |
| spellingShingle | Michael C. Roy Hiroki Nakanishi Kazuteru Takahashi Setsuko Nakanishi Shigeki Kajihara Takahiro Hayasaka Mitsutoshi Setou Kiyoshi Ogawa Ryo Taguchi Takayuki Naito Salamander retina phospholipids and their localization by MALDI imaging mass spectrometry at cellular size resolution[S] Journal of Lipid Research matrix-assisted laser desorption/ionization Ambystoma mexicanum lipidomics phospholipid |
| title | Salamander retina phospholipids and their localization by MALDI imaging mass spectrometry at cellular size resolution[S] |
| title_full | Salamander retina phospholipids and their localization by MALDI imaging mass spectrometry at cellular size resolution[S] |
| title_fullStr | Salamander retina phospholipids and their localization by MALDI imaging mass spectrometry at cellular size resolution[S] |
| title_full_unstemmed | Salamander retina phospholipids and their localization by MALDI imaging mass spectrometry at cellular size resolution[S] |
| title_short | Salamander retina phospholipids and their localization by MALDI imaging mass spectrometry at cellular size resolution[S] |
| title_sort | salamander retina phospholipids and their localization by maldi imaging mass spectrometry at cellular size resolution s |
| topic | matrix-assisted laser desorption/ionization Ambystoma mexicanum lipidomics phospholipid |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520409265 |
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