Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells
Epifluorescence microscopy (EFM) was used to study the biofilm formation of Pseudomonas aeruginosa after 6, 24, 30, 48, 54, 72, 78, and 96 h growth in a chamber slide system. For this purpose, the biofilm was stained with the Live/Dead BacLight, wherein live and dead cells were visualised based on t...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2016-06-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201603-0003_biofilm-formation-by-pseudomonas-aeruginosa-and-disinfectant-susceptibility-of-planktonic-and-biofilm-cells.php |
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| author | Magdalena A. Olszewska Aleksandra M. Kocot Aleksandra Stanowicka Łucja Łaniewska-Trokenheim |
| author_facet | Magdalena A. Olszewska Aleksandra M. Kocot Aleksandra Stanowicka Łucja Łaniewska-Trokenheim |
| author_sort | Magdalena A. Olszewska |
| collection | DOAJ |
| description | Epifluorescence microscopy (EFM) was used to study the biofilm formation of Pseudomonas aeruginosa after 6, 24, 30, 48, 54, 72, 78, and 96 h growth in a chamber slide system. For this purpose, the biofilm was stained with the Live/Dead BacLight, wherein live and dead cells were visualised based on the cell membrane integrity. With the use of EFM we described 8- of 9-stage biofilm characteristics after 78 h of growth, since the majority of microscopic fields were fully covered with attached cells. However, the 96-h growth resulted in the cell detachment and revealed 30% of dead cells of all those cells that remained on the surface. The susceptibility testing of planktonic and biofilm cells to two disinfectants, chlorine-based and quaternary ammonium compound-based, revealed that biofilm cells were more tolerant to a chlorine-based sanitiser than planktonic counterparts. P. aeruginosa was inhibited by lower concentrations of the quaternary ammonium compound-based sanitiser than the chlorine-based sanitiser, which on the other hand was more effective in cell inactivation, as both the MIC/MBC (inhibitory/bactericidal) measurement and the CFDA/PI (carboxyfluorescein diacetate/propidium iodide) staining indicated. |
| first_indexed | 2024-04-10T08:32:17Z |
| format | Article |
| id | doaj.art-0f7c69d2f56a48a098de9edc01c2e7de |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:32:17Z |
| publishDate | 2016-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-0f7c69d2f56a48a098de9edc01c2e7de2023-02-23T03:28:19ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172016-06-0134320421010.17221/528/2015-CJFScjf-201603-0003Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cellsMagdalena A. Olszewska0Aleksandra M. Kocot1Aleksandra Stanowicka2Łucja Łaniewska-Trokenheim3Chair of Industrial and Food Microbiology, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandChair of Industrial and Food Microbiology, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandChair of Industrial and Food Microbiology, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandChair of Industrial and Food Microbiology, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandEpifluorescence microscopy (EFM) was used to study the biofilm formation of Pseudomonas aeruginosa after 6, 24, 30, 48, 54, 72, 78, and 96 h growth in a chamber slide system. For this purpose, the biofilm was stained with the Live/Dead BacLight, wherein live and dead cells were visualised based on the cell membrane integrity. With the use of EFM we described 8- of 9-stage biofilm characteristics after 78 h of growth, since the majority of microscopic fields were fully covered with attached cells. However, the 96-h growth resulted in the cell detachment and revealed 30% of dead cells of all those cells that remained on the surface. The susceptibility testing of planktonic and biofilm cells to two disinfectants, chlorine-based and quaternary ammonium compound-based, revealed that biofilm cells were more tolerant to a chlorine-based sanitiser than planktonic counterparts. P. aeruginosa was inhibited by lower concentrations of the quaternary ammonium compound-based sanitiser than the chlorine-based sanitiser, which on the other hand was more effective in cell inactivation, as both the MIC/MBC (inhibitory/bactericidal) measurement and the CFDA/PI (carboxyfluorescein diacetate/propidium iodide) staining indicated.https://cjfs.agriculturejournals.cz/artkey/cjf-201603-0003_biofilm-formation-by-pseudomonas-aeruginosa-and-disinfectant-susceptibility-of-planktonic-and-biofilm-cells.phpmode of existencedisinfectionfluorescence microscopy |
| spellingShingle | Magdalena A. Olszewska Aleksandra M. Kocot Aleksandra Stanowicka Łucja Łaniewska-Trokenheim Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells Czech Journal of Food Sciences mode of existence disinfection fluorescence microscopy |
| title | Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells |
| title_full | Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells |
| title_fullStr | Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells |
| title_full_unstemmed | Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells |
| title_short | Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells |
| title_sort | biofilm formation by pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells |
| topic | mode of existence disinfection fluorescence microscopy |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201603-0003_biofilm-formation-by-pseudomonas-aeruginosa-and-disinfectant-susceptibility-of-planktonic-and-biofilm-cells.php |
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