Identification of ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone and isolates harboring a novel mosaic penA gene in Chengdu in 2019–2020
Abstract Background Antimicrobial resistance in gonorrhea has become a growing global public health burden. Neisseria gonorrhoeae isolates with resistance to ceftriaxone, the last remaining first-line option, represent an emerging threat of untreatable gonorrhea. Methods A total of ten ceftriaxone-r...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12941-023-00614-x |
| _version_ | 1797453590592225280 |
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| author | Di Wang Youwei Wang Yamei Li Leshan Xiu Gang Yong Yang Yang Weiming Gu Junping Peng |
| author_facet | Di Wang Youwei Wang Yamei Li Leshan Xiu Gang Yong Yang Yang Weiming Gu Junping Peng |
| author_sort | Di Wang |
| collection | DOAJ |
| description | Abstract Background Antimicrobial resistance in gonorrhea has become a growing global public health burden. Neisseria gonorrhoeae isolates with resistance to ceftriaxone, the last remaining first-line option, represent an emerging threat of untreatable gonorrhea. Methods A total of ten ceftriaxone-resistant N. gonorrhoeae FC428 isolates and two isolates harboring a novel mosaic penA-232.001 allele from 160 gonococcal isolates in Chengdu in 2019–2020 was described in the present study. Multilocus sequence typing (MLST) and N. gonorrhoeae sequence typing for antimicrobial resistance (NG-STAR) were performed to characterize the isolates. Whole genome sequencing and maximum-likelihood method were performed to infer how the genetic phylogenetic tree of these isolates looks like. Recombination analysis was performed using the RDP4 software. This study was registered in the Chinese Clinical Trial Registry (ChiCTR2100048771, registration date: 20210716). Results The genetic phylogeny showed that the ten FC428 isolates sporadically clustered into different phylogenetic clades, suggesting different introductions and local transmission of FC428. Two isolates showed close genetic relatedness to ceftriaxone-resistant clone A8806, which was only reported from Australia in 2013. Homologous recombination events were detected in penA between Neisseria gonorrhoeae and commensal Neisseria species (N. perflava and N. polysaccharea), providing evidence of commensal Neisseria species might serve as reservoirs of ceftriaxone resistance-mediating penA sequences in clinical gonococcal strains. Conclusions Our results demonstrate further dissemination of FC428 in China and resurgence risks of sporadic ceftriaxone-resistant A8806 to become the next clone to spread. |
| first_indexed | 2024-03-09T15:25:01Z |
| format | Article |
| id | doaj.art-0f7d62aad08a4919bf286a23768df973 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-03-09T15:25:01Z |
| publishDate | 2023-08-01 |
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| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-0f7d62aad08a4919bf286a23768df9732023-11-26T12:34:41ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112023-08-012211610.1186/s12941-023-00614-xIdentification of ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone and isolates harboring a novel mosaic penA gene in Chengdu in 2019–2020Di Wang0Youwei Wang1Yamei Li2Leshan Xiu3Gang Yong4Yang Yang5Weiming Gu6Junping Peng7NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical CollegeInstitute of Dermatology and Venereology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s HospitalNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical CollegeNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical CollegeInstitute of Dermatology and Venereology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s HospitalShanghai Skin Disease Hospital, Tongji University School of MedicineShanghai Skin Disease Hospital, Tongji University School of MedicineNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical CollegeAbstract Background Antimicrobial resistance in gonorrhea has become a growing global public health burden. Neisseria gonorrhoeae isolates with resistance to ceftriaxone, the last remaining first-line option, represent an emerging threat of untreatable gonorrhea. Methods A total of ten ceftriaxone-resistant N. gonorrhoeae FC428 isolates and two isolates harboring a novel mosaic penA-232.001 allele from 160 gonococcal isolates in Chengdu in 2019–2020 was described in the present study. Multilocus sequence typing (MLST) and N. gonorrhoeae sequence typing for antimicrobial resistance (NG-STAR) were performed to characterize the isolates. Whole genome sequencing and maximum-likelihood method were performed to infer how the genetic phylogenetic tree of these isolates looks like. Recombination analysis was performed using the RDP4 software. This study was registered in the Chinese Clinical Trial Registry (ChiCTR2100048771, registration date: 20210716). Results The genetic phylogeny showed that the ten FC428 isolates sporadically clustered into different phylogenetic clades, suggesting different introductions and local transmission of FC428. Two isolates showed close genetic relatedness to ceftriaxone-resistant clone A8806, which was only reported from Australia in 2013. Homologous recombination events were detected in penA between Neisseria gonorrhoeae and commensal Neisseria species (N. perflava and N. polysaccharea), providing evidence of commensal Neisseria species might serve as reservoirs of ceftriaxone resistance-mediating penA sequences in clinical gonococcal strains. Conclusions Our results demonstrate further dissemination of FC428 in China and resurgence risks of sporadic ceftriaxone-resistant A8806 to become the next clone to spread.https://doi.org/10.1186/s12941-023-00614-xNeisseria gonorrhoeaeCeftriaxone resistanceFC428A8806Homologous recombination |
| spellingShingle | Di Wang Youwei Wang Yamei Li Leshan Xiu Gang Yong Yang Yang Weiming Gu Junping Peng Identification of ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone and isolates harboring a novel mosaic penA gene in Chengdu in 2019–2020 Annals of Clinical Microbiology and Antimicrobials Neisseria gonorrhoeae Ceftriaxone resistance FC428 A8806 Homologous recombination |
| title | Identification of ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone and isolates harboring a novel mosaic penA gene in Chengdu in 2019–2020 |
| title_full | Identification of ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone and isolates harboring a novel mosaic penA gene in Chengdu in 2019–2020 |
| title_fullStr | Identification of ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone and isolates harboring a novel mosaic penA gene in Chengdu in 2019–2020 |
| title_full_unstemmed | Identification of ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone and isolates harboring a novel mosaic penA gene in Chengdu in 2019–2020 |
| title_short | Identification of ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone and isolates harboring a novel mosaic penA gene in Chengdu in 2019–2020 |
| title_sort | identification of ceftriaxone resistant neisseria gonorrhoeae fc428 clone and isolates harboring a novel mosaic pena gene in chengdu in 2019 2020 |
| topic | Neisseria gonorrhoeae Ceftriaxone resistance FC428 A8806 Homologous recombination |
| url | https://doi.org/10.1186/s12941-023-00614-x |
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