Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology
Abstract To improve the fermentation efficiency of Propionibacterium acidipropionici, a semi‐continuous coupled fermentation process was established to achieve co‐production of propionic acid (PA) and succinic acid (SA). First, the optimal proportion of glucose (Glc) and glycerol (Gl) as a mixed car...
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| Format: | Article |
| Sprog: | English |
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Wiley-VCH
2021-06-01
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| Serier: | Engineering in Life Sciences |
| Fag: | |
| Online adgang: | https://doi.org/10.1002/elsc.202000103 |
| _version_ | 1830308245869166592 |
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| author | Xiaolian Li Liquan Wei Ziqiang Wang Yunshan Wang Zhiguo Su |
| author_facet | Xiaolian Li Liquan Wei Ziqiang Wang Yunshan Wang Zhiguo Su |
| author_sort | Xiaolian Li |
| collection | DOAJ |
| description | Abstract To improve the fermentation efficiency of Propionibacterium acidipropionici, a semi‐continuous coupled fermentation process was established to achieve co‐production of propionic acid (PA) and succinic acid (SA). First, the optimal proportion of glucose (Glc) and glycerol (Gl) as a mixed carbon source was determined, and the feeding procedure of Gl was optimized to make more energy flow in the direction of product synthesis. Then, ZGD630 anion exchange resin was used for efficient adsorption of PA, thereby eliminating the feedback inhibition effect of PA. Finally, an efficient, coupled fermentation process of P. acidipropionici characterized by membrane separation and chromatography technology was developed. The concentrations of PA and SA reached 62.22 ± 2.32 and 20.45 ± 1.34 g L−1, with corresponding productivity of 0.43 and 0.14 g L−1 h−1, increased by 65.38% and 48.54%, respectively. Membrane separation coupled fermentation of PA and SA could significantly improve the process economics of P. acidipropionici, and has good prospects for industrial application. |
| first_indexed | 2024-12-19T10:46:38Z |
| format | Article |
| id | doaj.art-0f800c7db10343fd9d1d3edcb1bac7ba |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-12-19T10:46:38Z |
| publishDate | 2021-06-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-0f800c7db10343fd9d1d3edcb1bac7ba2022-12-21T20:25:15ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632021-06-0121642943710.1002/elsc.202000103Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technologyXiaolian Li0Liquan Wei1Ziqiang Wang2Yunshan Wang3Zhiguo Su4State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. ChinaState Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. ChinaState Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. ChinaState Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. ChinaState Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. ChinaAbstract To improve the fermentation efficiency of Propionibacterium acidipropionici, a semi‐continuous coupled fermentation process was established to achieve co‐production of propionic acid (PA) and succinic acid (SA). First, the optimal proportion of glucose (Glc) and glycerol (Gl) as a mixed carbon source was determined, and the feeding procedure of Gl was optimized to make more energy flow in the direction of product synthesis. Then, ZGD630 anion exchange resin was used for efficient adsorption of PA, thereby eliminating the feedback inhibition effect of PA. Finally, an efficient, coupled fermentation process of P. acidipropionici characterized by membrane separation and chromatography technology was developed. The concentrations of PA and SA reached 62.22 ± 2.32 and 20.45 ± 1.34 g L−1, with corresponding productivity of 0.43 and 0.14 g L−1 h−1, increased by 65.38% and 48.54%, respectively. Membrane separation coupled fermentation of PA and SA could significantly improve the process economics of P. acidipropionici, and has good prospects for industrial application.https://doi.org/10.1002/elsc.202000103combination of glucose and glycerolmembrane separation coupled fermentationPropionibacterium acidipropionicipropionic acidsuccinic acid |
| spellingShingle | Xiaolian Li Liquan Wei Ziqiang Wang Yunshan Wang Zhiguo Su Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology Engineering in Life Sciences combination of glucose and glycerol membrane separation coupled fermentation Propionibacterium acidipropionici propionic acid succinic acid |
| title | Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology |
| title_full | Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology |
| title_fullStr | Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology |
| title_full_unstemmed | Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology |
| title_short | Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology |
| title_sort | efficient co production of propionic acid and succinic acid by propionibacterium acidipropionici using membrane separation coupled technology |
| topic | combination of glucose and glycerol membrane separation coupled fermentation Propionibacterium acidipropionici propionic acid succinic acid |
| url | https://doi.org/10.1002/elsc.202000103 |
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