Gas Chromatography/Mass Spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from Clusia minor L.
Context: The search of new substances with analgesic properties has grown in the last years. Brazil and Cuba have a big biodiversity allowing the study of several plants with potential pharmacological activities. Aims: To evaluate the chemical composition and potential antinociceptive effect of t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
GarVal Editorial Ltda.
2019-01-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol7/jppres18.432_7.1.21.pdf |
| _version_ | 1811322021493080064 |
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| author | Raisa Mangas Gledys Reynaldo Maria T. Dalla Vecchia Kendely Aver Leonardo G. Piovesan Adonis Bello Idania Rodeiro Angela Malheiros Marcia M. de Souza Roberto Menéndez |
| author_facet | Raisa Mangas Gledys Reynaldo Maria T. Dalla Vecchia Kendely Aver Leonardo G. Piovesan Adonis Bello Idania Rodeiro Angela Malheiros Marcia M. de Souza Roberto Menéndez |
| author_sort | Raisa Mangas |
| collection | DOAJ |
| description | Context: The search of new substances with analgesic properties has grown in the last years. Brazil and Cuba have a big biodiversity allowing the study of several plants with potential pharmacological activities.
Aims: To evaluate the chemical composition and potential antinociceptive effect of the ethanolic extract from Clusia minor L. leaves (Clusiaceae) in mice.
Methods: Phytochemical characterization was performed by Gas Chromatography/Mass Spectrometry. Antinociceptive effect was evaluated using acetic acid, formalin, hot plate, and capsaicin models. Mechanical hypernociception was induced by intraplantar carrageenan, tumor necrosis factor α (TNFα) and prostagladin E2 (PGE2) and responses were measured after 3 h of injection.
Results: Mass Spectrometry analysis allowed the identification of 16 compounds. Fatty acid derivatives, steroids, triterpenoids, and vitamin E were the main findings. The most abundant sterol was β-sitosterol (14.04%); followed by the triterpenes α-amyrin (11.94%), and β-amyrin (7.82%). Vitamin E represented the 8.44% of the total identified compounds. The evaluation of the acetic acid-induced nociception model showed that the extract was effective in reducing pain in a dose-dependent manner. This resulted in a maximal inhibition of 53 ± 4%. The extract was also effective in other pain models. Additionally, the extract presented a considerable inhibition of paw mechanical hypernociception.
Conclusions: The data suggest that the antinociceptive effect of Clusia minor occurs by interaction of various mechanisms; which probably take places via central and peripheral pathway. Therefore, modulating the inflammatory and neurogenic pain. |
| first_indexed | 2024-04-13T13:27:52Z |
| format | Article |
| id | doaj.art-0f84d4a69542482aba9b4f8ed816f24c |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-04-13T13:27:52Z |
| publishDate | 2019-01-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-0f84d4a69542482aba9b4f8ed816f24c2022-12-22T02:45:04ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502019-01-01712130Gas Chromatography/Mass Spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from Clusia minor L.Raisa Mangas0Gledys Reynaldo1Maria T. Dalla Vecchia2Kendely Aver3Leonardo G. Piovesan4Adonis Bello5Idania Rodeiro6Angela Malheiros7Marcia M. de Souza8Roberto Menéndez9Department of Pharmacy, Institute of Pharmacy and Food (IFAL), University of Havana, 23 Ave., 21425, La Coronela, La Lisa, Havana, Cuba.Department of Pharmacy, Institute of Pharmacy and Food (IFAL), University of Havana, 23 Ave., 21425, La Coronela, La Lisa, Havana, Cuba.Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, Brazil.Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, Brazil.Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, Brazil.Faculty of Chemical Sciences, University of Guayaquil, Ciudadela Universitaria Dr. Salvador Allende, Guayaquil, Ecuador.Department of Pharmacology, Institute of Marine Sciences (ICIMAR), Loma and 37, Vedado, Havana, Cuba.Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, Brazil.Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, Brazil.Cuban Neurosciences Center, 25 Ave., 5202, Cubanacan, Playa, Havana, Cuba.Context: The search of new substances with analgesic properties has grown in the last years. Brazil and Cuba have a big biodiversity allowing the study of several plants with potential pharmacological activities. Aims: To evaluate the chemical composition and potential antinociceptive effect of the ethanolic extract from Clusia minor L. leaves (Clusiaceae) in mice. Methods: Phytochemical characterization was performed by Gas Chromatography/Mass Spectrometry. Antinociceptive effect was evaluated using acetic acid, formalin, hot plate, and capsaicin models. Mechanical hypernociception was induced by intraplantar carrageenan, tumor necrosis factor α (TNFα) and prostagladin E2 (PGE2) and responses were measured after 3 h of injection. Results: Mass Spectrometry analysis allowed the identification of 16 compounds. Fatty acid derivatives, steroids, triterpenoids, and vitamin E were the main findings. The most abundant sterol was β-sitosterol (14.04%); followed by the triterpenes α-amyrin (11.94%), and β-amyrin (7.82%). Vitamin E represented the 8.44% of the total identified compounds. The evaluation of the acetic acid-induced nociception model showed that the extract was effective in reducing pain in a dose-dependent manner. This resulted in a maximal inhibition of 53 ± 4%. The extract was also effective in other pain models. Additionally, the extract presented a considerable inhibition of paw mechanical hypernociception. Conclusions: The data suggest that the antinociceptive effect of Clusia minor occurs by interaction of various mechanisms; which probably take places via central and peripheral pathway. Therefore, modulating the inflammatory and neurogenic pain.http://jppres.com/jppres/pdf/vol7/jppres18.432_7.1.21.pdfacute mechanical hypernociceptionacute nociceptive modelsantinociceptive effectClusia minor L.GC/MStriterpenoids |
| spellingShingle | Raisa Mangas Gledys Reynaldo Maria T. Dalla Vecchia Kendely Aver Leonardo G. Piovesan Adonis Bello Idania Rodeiro Angela Malheiros Marcia M. de Souza Roberto Menéndez Gas Chromatography/Mass Spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from Clusia minor L. Journal of Pharmacy & Pharmacognosy Research acute mechanical hypernociception acute nociceptive models antinociceptive effect Clusia minor L. GC/MS triterpenoids |
| title | Gas Chromatography/Mass Spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from Clusia minor L. |
| title_full | Gas Chromatography/Mass Spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from Clusia minor L. |
| title_fullStr | Gas Chromatography/Mass Spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from Clusia minor L. |
| title_full_unstemmed | Gas Chromatography/Mass Spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from Clusia minor L. |
| title_short | Gas Chromatography/Mass Spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from Clusia minor L. |
| title_sort | gas chromatography mass spectrometry characterization and antinociceptive effects of the ethanolic extract of the leaves from clusia minor l |
| topic | acute mechanical hypernociception acute nociceptive models antinociceptive effect Clusia minor L. GC/MS triterpenoids |
| url | http://jppres.com/jppres/pdf/vol7/jppres18.432_7.1.21.pdf |
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