Binding of 99mTc to human serum albumin
The specificity of 99m Tc-MDP binding to HSA was examined , the results showed that the non specific binding was 21-23% of the total binding . The range of optimum pH for the binding was 7.4-7.6 . The most suitable amounts for HSA to give maximum binding was (70mg)per incubation medium , binding se...
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| Format: | Article |
| Language: | English |
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College of Pharmacy / Mustansiriyah University
2004-06-01
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| Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/449 |
| _version_ | 1797223835875934208 |
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| author | Abdulrahman K. Loay |
| author_facet | Abdulrahman K. Loay |
| author_sort | Abdulrahman K. Loay |
| collection | DOAJ |
| description |
The specificity of 99m Tc-MDP binding to HSA was examined , the results showed that the non specific binding was 21-23% of the total binding . The range of optimum pH for the binding was 7.4-7.6 . The most suitable amounts for HSA to give maximum binding was (70mg)per incubation medium , binding seemed to be affected by 99m Tc- MDP concentration and also mono and divalent metal chloride salts. The association constants (ka) and maximum binding capacity (Bmax) of 99m Tc-MDP binding to its binding sites of HSA was determined from scatchard and double reciprocal plots , the results revealed that (ka) and (Bmax) values were temperature dependant.
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| first_indexed | 2024-04-24T13:43:33Z |
| format | Article |
| id | doaj.art-0f86f3933b7f4c429a47139602cacc5e |
| institution | Directory Open Access Journal |
| issn | 1815-0993 2959-183X |
| language | English |
| last_indexed | 2024-04-24T13:43:33Z |
| publishDate | 2004-06-01 |
| publisher | College of Pharmacy / Mustansiriyah University |
| record_format | Article |
| series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| spelling | doaj.art-0f86f3933b7f4c429a47139602cacc5e2024-04-04T06:59:28ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2004-06-011110.32947/ajps.v1i1.449Binding of 99mTc to human serum albuminAbdulrahman K. Loay The specificity of 99m Tc-MDP binding to HSA was examined , the results showed that the non specific binding was 21-23% of the total binding . The range of optimum pH for the binding was 7.4-7.6 . The most suitable amounts for HSA to give maximum binding was (70mg)per incubation medium , binding seemed to be affected by 99m Tc- MDP concentration and also mono and divalent metal chloride salts. The association constants (ka) and maximum binding capacity (Bmax) of 99m Tc-MDP binding to its binding sites of HSA was determined from scatchard and double reciprocal plots , the results revealed that (ka) and (Bmax) values were temperature dependant. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/449 |
| spellingShingle | Abdulrahman K. Loay Binding of 99mTc to human serum albumin Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| title | Binding of 99mTc to human serum albumin |
| title_full | Binding of 99mTc to human serum albumin |
| title_fullStr | Binding of 99mTc to human serum albumin |
| title_full_unstemmed | Binding of 99mTc to human serum albumin |
| title_short | Binding of 99mTc to human serum albumin |
| title_sort | binding of 99mtc to human serum albumin |
| url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/449 |
| work_keys_str_mv | AT abdulrahmankloay bindingof99mtctohumanserumalbumin |