Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in Dogs
Objective- To evaluate the effects of autologous platelet-rich plasma (PRP) on wound healing in dogs. Design- Experimental in-vivo study. Animals- 5 mix-breed male adult dogs. Procedures- Under general anesthesia, six 2 × 2-cm, full-thickness skin wounds were created on the back of dogs symmetricall...
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| Format: | Article |
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Iranian Veterinary Surgery Association (IVSA)
2008-12-01
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| Series: | Iranian Journal of Veterinary Surgery |
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| Online Access: | http://www.ivsajournals.com/article_3163_5dd04cf1f7259160e3204a09555c5946.pdf |
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| author | Hossein Kazemi Mehrjerdi Kamran Sardari Mohamad Reza Emami Ahmad Reza Movassaghi Amir Afkhami Goli Abbas Lotfi Sara Malekzadeh |
| author_facet | Hossein Kazemi Mehrjerdi Kamran Sardari Mohamad Reza Emami Ahmad Reza Movassaghi Amir Afkhami Goli Abbas Lotfi Sara Malekzadeh |
| author_sort | Hossein Kazemi Mehrjerdi |
| collection | DOAJ |
| description | Objective- To evaluate the effects of autologous platelet-rich plasma (PRP) on wound healing in dogs.
Design- Experimental in-vivo study.
Animals- 5 mix-breed male adult dogs.
Procedures- Under general anesthesia, six 2 × 2-cm, full-thickness skin wounds were created on the back of dogs symmetrically. In each dog, three right side wounds were treated topically with 1.5 ml PRP jelly, whereas left side wounds received no treatment. For macroscopic evaluation, at days 0, 3, 5, 7, 10, 13, 17, 20, and 24, digital photographs were taken from wounds. At days 10, 17 and 24 after wounding, skin biopsies were taken from the center and corner of each wound for hydroxyprolin measurement and histopathologic evaluation respectively.
Results- No statistically significant differences were found in percentage of wound contraction, epithelialization and healing between test and control group during study (P>0.05). There were no significant differences between median of hydroxyprolin levels, median of inflammatory cells infiltration, presence of dermal granulation tissue, fibroblast proliferation, arrangement of fibroblasts, collagen deposition and collagen bundle formation scores, in the specimens from left and right wounds (P>0.05).
Conclusion and Clinical Relevance- Topical application of platelet-rich plasma can not accelerate repair of the small granulating wounds of dogs. |
| first_indexed | 2024-12-16T11:49:38Z |
| format | Article |
| id | doaj.art-0f89abcd7efa46a0bd706e2b954d5362 |
| institution | Directory Open Access Journal |
| issn | 2008-3033 2008-3033 |
| language | English |
| last_indexed | 2024-12-16T11:49:38Z |
| publishDate | 2008-12-01 |
| publisher | Iranian Veterinary Surgery Association (IVSA) |
| record_format | Article |
| series | Iranian Journal of Veterinary Surgery |
| spelling | doaj.art-0f89abcd7efa46a0bd706e2b954d53622022-12-21T22:32:45ZengIranian Veterinary Surgery Association (IVSA)Iranian Journal of Veterinary Surgery2008-30332008-30332008-12-0103419303163Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in DogsHossein Kazemi Mehrjerdi0Kamran Sardari1Mohamad Reza Emami2Ahmad Reza Movassaghi3Amir Afkhami Goli4Abbas Lotfi5Sara Malekzadeh6Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Graduated Student, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Graduated Student, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Objective- To evaluate the effects of autologous platelet-rich plasma (PRP) on wound healing in dogs. Design- Experimental in-vivo study. Animals- 5 mix-breed male adult dogs. Procedures- Under general anesthesia, six 2 × 2-cm, full-thickness skin wounds were created on the back of dogs symmetrically. In each dog, three right side wounds were treated topically with 1.5 ml PRP jelly, whereas left side wounds received no treatment. For macroscopic evaluation, at days 0, 3, 5, 7, 10, 13, 17, 20, and 24, digital photographs were taken from wounds. At days 10, 17 and 24 after wounding, skin biopsies were taken from the center and corner of each wound for hydroxyprolin measurement and histopathologic evaluation respectively. Results- No statistically significant differences were found in percentage of wound contraction, epithelialization and healing between test and control group during study (P>0.05). There were no significant differences between median of hydroxyprolin levels, median of inflammatory cells infiltration, presence of dermal granulation tissue, fibroblast proliferation, arrangement of fibroblasts, collagen deposition and collagen bundle formation scores, in the specimens from left and right wounds (P>0.05). Conclusion and Clinical Relevance- Topical application of platelet-rich plasma can not accelerate repair of the small granulating wounds of dogs.http://www.ivsajournals.com/article_3163_5dd04cf1f7259160e3204a09555c5946.pdfDogwound healingPlatelet- rich plasmaGrowth Factors |
| spellingShingle | Hossein Kazemi Mehrjerdi Kamran Sardari Mohamad Reza Emami Ahmad Reza Movassaghi Amir Afkhami Goli Abbas Lotfi Sara Malekzadeh Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in Dogs Iranian Journal of Veterinary Surgery Dog wound healing Platelet- rich plasma Growth Factors |
| title | Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in Dogs |
| title_full | Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in Dogs |
| title_fullStr | Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in Dogs |
| title_full_unstemmed | Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in Dogs |
| title_short | Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in Dogs |
| title_sort | efficacy of autologous platelet rich plasma prp activated by thromboplastin d on the repair and regeneration of wounds in dogs |
| topic | Dog wound healing Platelet- rich plasma Growth Factors |
| url | http://www.ivsajournals.com/article_3163_5dd04cf1f7259160e3204a09555c5946.pdf |
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