A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1
Here, the authors report the discovery of a redox switch for AcrIIC1, which regulates AcrIIC1’s monomer-dimer interconversion and inhibitory activity on Cas9. By reporting the structures of different redox states of NmeAcrIIC1, they identify a pair of conserved cysteines which mediate the redox swit...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34551-8 |
| _version_ | 1798016800584105984 |
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| author | Yanan Zhao Jiaojiao Hu Shan-Shan Yang Jing Zhong Jianping Liu Shuo Wang Yuzhuo Jiao Fang Jiang Ruiyang Zhai Bingnan Ren Hua Cong Yuwei Zhu Fengtong Han Jixian Zhang Yue Xu Zhiwei Huang Shengnan Zhang Fan Yang |
| author_facet | Yanan Zhao Jiaojiao Hu Shan-Shan Yang Jing Zhong Jianping Liu Shuo Wang Yuzhuo Jiao Fang Jiang Ruiyang Zhai Bingnan Ren Hua Cong Yuwei Zhu Fengtong Han Jixian Zhang Yue Xu Zhiwei Huang Shengnan Zhang Fan Yang |
| author_sort | Yanan Zhao |
| collection | DOAJ |
| description | Here, the authors report the discovery of a redox switch for AcrIIC1, which regulates AcrIIC1’s monomer-dimer interconversion and inhibitory activity on Cas9. By reporting the structures of different redox states of NmeAcrIIC1, they identify a pair of conserved cysteines which mediate the redox switch. The replacement of the redox-sensitive cysteines allows the generation of a more stable and robust variant under various cellular environments. |
| first_indexed | 2024-04-11T15:56:30Z |
| format | Article |
| id | doaj.art-0f8c3e9d88fa475ab4d7f296eddd69ce |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T15:56:30Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-0f8c3e9d88fa475ab4d7f296eddd69ce2022-12-22T04:15:08ZengNature PortfolioNature Communications2041-17232022-11-0113111310.1038/s41467-022-34551-8A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1Yanan Zhao0Jiaojiao Hu1Shan-Shan Yang2Jing Zhong3Jianping Liu4Shuo Wang5Yuzhuo Jiao6Fang Jiang7Ruiyang Zhai8Bingnan Ren9Hua Cong10Yuwei Zhu11Fengtong Han12Jixian Zhang13Yue Xu14Zhiwei Huang15Shengnan Zhang16Fan Yang17School of Life Science and Technology, Harbin Institute of TechnologyInterdisciplinary Research Center on Biology and Chemistry, Shanghai institute of Organic Chemistry, Chinese Academy of SciencesState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of TechnologySchool of Life Science and Technology, Harbin Institute of TechnologyInterdisciplinary Research Center on Biology and Chemistry, Shanghai institute of Organic Chemistry, Chinese Academy of SciencesHIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of TechnologySchool of Life Science and Technology, Harbin Institute of TechnologySchool of Life Science and Technology, Harbin Institute of TechnologySchool of Life Science and Technology, Harbin Institute of TechnologySchool of Life Science and Technology, Harbin Institute of TechnologySchool of Life Science and Technology, Harbin Institute of TechnologyHIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of TechnologySchool of Life Science and Technology, Harbin Institute of TechnologySchool of Life Science and Technology, Harbin Institute of TechnologySchool of Life Science and Technology, Harbin Institute of TechnologyHIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of TechnologyInterdisciplinary Research Center on Biology and Chemistry, Shanghai institute of Organic Chemistry, Chinese Academy of SciencesSchool of Life Science and Technology, Harbin Institute of TechnologyHere, the authors report the discovery of a redox switch for AcrIIC1, which regulates AcrIIC1’s monomer-dimer interconversion and inhibitory activity on Cas9. By reporting the structures of different redox states of NmeAcrIIC1, they identify a pair of conserved cysteines which mediate the redox switch. The replacement of the redox-sensitive cysteines allows the generation of a more stable and robust variant under various cellular environments.https://doi.org/10.1038/s41467-022-34551-8 |
| spellingShingle | Yanan Zhao Jiaojiao Hu Shan-Shan Yang Jing Zhong Jianping Liu Shuo Wang Yuzhuo Jiao Fang Jiang Ruiyang Zhai Bingnan Ren Hua Cong Yuwei Zhu Fengtong Han Jixian Zhang Yue Xu Zhiwei Huang Shengnan Zhang Fan Yang A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1 Nature Communications |
| title | A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1 |
| title_full | A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1 |
| title_fullStr | A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1 |
| title_full_unstemmed | A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1 |
| title_short | A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1 |
| title_sort | redox switch regulates the assembly and anti crispr activity of acriic1 |
| url | https://doi.org/10.1038/s41467-022-34551-8 |
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