Response characteristics of rhizosphere microbial community and metabolites of Iris tectorum to Cr stress
Chromium (Cr) is a toxic heavy element that interferes with plant metabolite biosynthesis and modifies the plant rhizosphere microenvironment, affecting plant growth. However, the interactions and response mechanisms between plants and rhizosphere bacteria under Cr stress still need to be fully unde...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323007224 |
| _version_ | 1797683180929548288 |
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| author | Luying Sheng Wei Zhao Xiuqin Yang Huan Mao Sixi Zhu |
| author_facet | Luying Sheng Wei Zhao Xiuqin Yang Huan Mao Sixi Zhu |
| author_sort | Luying Sheng |
| collection | DOAJ |
| description | Chromium (Cr) is a toxic heavy element that interferes with plant metabolite biosynthesis and modifies the plant rhizosphere microenvironment, affecting plant growth. However, the interactions and response mechanisms between plants and rhizosphere bacteria under Cr stress still need to be fully understood. In this study, we used Iris tectorum as a research target and combined physiology, metabolomics, and microbiology to reveal the stress response mechanism of I. tectorum under heavy metal chromium stress. The results showed that Cr stress-induced oxidative stress inhibited plant growth and development and increased malondialdehyde and oxygen free radicals content. Also, it increased ascorbate peroxidase, peroxidase activity, and superoxide dismutase activity, as well as glutathione and soluble sugar content. Microbiome analysis showed that Cr stress changed the rhizosphere bacterial community diversity index by 33.56%. Proteobacteria, Actinobacteriota, and Chloroflexi together accounting for 71.21% of the total sequences. Meanwhile, the abundance of rhizosphere dominant and plant-promoting bacteria increased significantly with increasing time of Cr stress. The improvement of the soil microenvironment and the recruitment of bacteria by I. tectorum root secretions were significantly enhanced. By metabolomic analysis, five vital metabolic pathways were identified, involving 89 differentially expressed metabolites, divided into 15 major categories. In summary, a multi-omics approach was used in this study to reveal the interaction and stress response mechanisms between I. tectorum and rhizosphere bacterial communities under Cr stress, which provided theoretical basis for plant-microbial bioremediation of Cr-contaminated soils in constructed wetlands. This may provide more valuable information for wetland remediation of heavy metal pollution. |
| first_indexed | 2024-03-12T00:10:47Z |
| format | Article |
| id | doaj.art-0f8f27b78231450582811419a11b270c |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-12T00:10:47Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-0f8f27b78231450582811419a11b270c2023-09-16T05:28:12ZengElsevierEcotoxicology and Environmental Safety0147-65132023-09-01263115218Response characteristics of rhizosphere microbial community and metabolites of Iris tectorum to Cr stressLuying Sheng0Wei Zhao1Xiuqin Yang2Huan Mao3Sixi Zhu4College of Eco-environment Engineering, Guizhou Minzu University, China; The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, Guiyang 550025, ChinaCollege of Eco-environment Engineering, Guizhou Minzu University, China; The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, Guiyang 550025, ChinaCollege of Eco-environment Engineering, Guizhou Minzu University, ChinaCollege of Eco-environment Engineering, Guizhou Minzu University, China; The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, Guiyang 550025, ChinaCollege of Eco-environment Engineering, Guizhou Minzu University, China; The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, Guiyang 550025, China; Correspondence to: College of Eco-environment Engineering, Guizhou Minzu University, Guiyang 550025, China.Chromium (Cr) is a toxic heavy element that interferes with plant metabolite biosynthesis and modifies the plant rhizosphere microenvironment, affecting plant growth. However, the interactions and response mechanisms between plants and rhizosphere bacteria under Cr stress still need to be fully understood. In this study, we used Iris tectorum as a research target and combined physiology, metabolomics, and microbiology to reveal the stress response mechanism of I. tectorum under heavy metal chromium stress. The results showed that Cr stress-induced oxidative stress inhibited plant growth and development and increased malondialdehyde and oxygen free radicals content. Also, it increased ascorbate peroxidase, peroxidase activity, and superoxide dismutase activity, as well as glutathione and soluble sugar content. Microbiome analysis showed that Cr stress changed the rhizosphere bacterial community diversity index by 33.56%. Proteobacteria, Actinobacteriota, and Chloroflexi together accounting for 71.21% of the total sequences. Meanwhile, the abundance of rhizosphere dominant and plant-promoting bacteria increased significantly with increasing time of Cr stress. The improvement of the soil microenvironment and the recruitment of bacteria by I. tectorum root secretions were significantly enhanced. By metabolomic analysis, five vital metabolic pathways were identified, involving 89 differentially expressed metabolites, divided into 15 major categories. In summary, a multi-omics approach was used in this study to reveal the interaction and stress response mechanisms between I. tectorum and rhizosphere bacterial communities under Cr stress, which provided theoretical basis for plant-microbial bioremediation of Cr-contaminated soils in constructed wetlands. This may provide more valuable information for wetland remediation of heavy metal pollution.http://www.sciencedirect.com/science/article/pii/S0147651323007224Chromium pollutionAntioxidation mechanismRhizosphere bacterial community16 s rRNA gene sequencingMetabonomics |
| spellingShingle | Luying Sheng Wei Zhao Xiuqin Yang Huan Mao Sixi Zhu Response characteristics of rhizosphere microbial community and metabolites of Iris tectorum to Cr stress Ecotoxicology and Environmental Safety Chromium pollution Antioxidation mechanism Rhizosphere bacterial community 16 s rRNA gene sequencing Metabonomics |
| title | Response characteristics of rhizosphere microbial community and metabolites of Iris tectorum to Cr stress |
| title_full | Response characteristics of rhizosphere microbial community and metabolites of Iris tectorum to Cr stress |
| title_fullStr | Response characteristics of rhizosphere microbial community and metabolites of Iris tectorum to Cr stress |
| title_full_unstemmed | Response characteristics of rhizosphere microbial community and metabolites of Iris tectorum to Cr stress |
| title_short | Response characteristics of rhizosphere microbial community and metabolites of Iris tectorum to Cr stress |
| title_sort | response characteristics of rhizosphere microbial community and metabolites of iris tectorum to cr stress |
| topic | Chromium pollution Antioxidation mechanism Rhizosphere bacterial community 16 s rRNA gene sequencing Metabonomics |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323007224 |
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