Effective Treatment of Patients Experiencing Primary, Acute HIV Infection Decreases Exhausted/Activated CD4+ T Cells and CD8+ T Memory Stem Cells
Several studies have identified main changes in T- and B-lymphocyte subsets during chronic HIV infection, but few data exist on how these subsets behave during the initial phase of HIV infection. We enrolled 22 HIV-infected patients during the acute stage of infection before the initiation of antire...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/15/2307 |
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| author | Domenico Lo Tartaro Antonio Camiro-Zúñiga Milena Nasi Sara De Biasi Marco A. Najera-Avila Maria Del Rocio Jaramillo-Jante Lara Gibellini Marcello Pinti Anita Neroni Cristina Mussini Luis E. Soto-Ramírez Juan J. Calva Francisco Belaunzarán-Zamudio Brenda Crabtree-Ramirez Christian Hernández-Leon Juan L. Mosqueda-Gómez Samuel Navarro-Álvarez Santiago Perez-Patrigeon Andrea Cossarizza |
| author_facet | Domenico Lo Tartaro Antonio Camiro-Zúñiga Milena Nasi Sara De Biasi Marco A. Najera-Avila Maria Del Rocio Jaramillo-Jante Lara Gibellini Marcello Pinti Anita Neroni Cristina Mussini Luis E. Soto-Ramírez Juan J. Calva Francisco Belaunzarán-Zamudio Brenda Crabtree-Ramirez Christian Hernández-Leon Juan L. Mosqueda-Gómez Samuel Navarro-Álvarez Santiago Perez-Patrigeon Andrea Cossarizza |
| author_sort | Domenico Lo Tartaro |
| collection | DOAJ |
| description | Several studies have identified main changes in T- and B-lymphocyte subsets during chronic HIV infection, but few data exist on how these subsets behave during the initial phase of HIV infection. We enrolled 22 HIV-infected patients during the acute stage of infection before the initiation of antiretroviral therapy (ART). Patients had blood samples drawn previous to ART initiation (T0), and at 2 (T1) and 12 (T2) months after ART initiation. We quantified cellular HIV-DNA content in sorted naïve and effector memory CD4 T cells and identified the main subsets of T- and B-lymphocytes using an 18-parameter flow cytometry panel. We identified correlations between the patients’ clinical and immunological data using PCA. Effective HIV treatment reduces integrated HIV DNA in effector memory T cells after 12 months (T2) of ART. The main changes in CD4+ T cells occurred at T2, with a reduction of activated memory, cytolytic and activated/exhausted stem cell memory T (T<sub>SCM</sub>) cells. Changes were present among CD8+ T cells since T1, with a reduction of several activated subsets, including activated/exhausted T<sub>SCM</sub>. At T2 a reduction of plasmablasts and exhausted B cells was also observed. A negative correlation was found between the total CD4+ T-cell count and IgM-negative plasmablasts. In patients initiating ART immediately following acute/early HIV infection, the fine analysis of T- and B-cell subsets has allowed us to identify and follow main modifications due to effective treatment, and to identify significant changes in CD4+ and CD8+ T memory stem cells. |
| first_indexed | 2024-03-09T05:30:56Z |
| format | Article |
| id | doaj.art-0f8f2ace8a154d6a94737af8d2be0fd6 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T05:30:56Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-0f8f2ace8a154d6a94737af8d2be0fd62023-12-03T12:32:02ZengMDPI AGCells2073-44092022-07-011115230710.3390/cells11152307Effective Treatment of Patients Experiencing Primary, Acute HIV Infection Decreases Exhausted/Activated CD4+ T Cells and CD8+ T Memory Stem CellsDomenico Lo Tartaro0Antonio Camiro-Zúñiga1Milena Nasi2Sara De Biasi3Marco A. Najera-Avila4Maria Del Rocio Jaramillo-Jante5Lara Gibellini6Marcello Pinti7Anita Neroni8Cristina Mussini9Luis E. Soto-Ramírez10Juan J. Calva11Francisco Belaunzarán-Zamudio12Brenda Crabtree-Ramirez13Christian Hernández-Leon14Juan L. Mosqueda-Gómez15Samuel Navarro-Álvarez16Santiago Perez-Patrigeon17Andrea Cossarizza18Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, 41125 Modena, ItalyInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Infectious Diseases, Mexico City 14080, MexicoDepartment of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, 41125 Modena, ItalyInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Infectious Diseases, Mexico City 14080, MexicoInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Infectious Diseases, Mexico City 14080, MexicoDepartment of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, 41125 Modena, ItalyDepartment of Life Sciences, University of Modena and Reggio Emilia, 41125 Modena, ItalyDepartment of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, 41125 Modena, ItalyInfectious Diseases Clinics, Azienda Ospedaliero-Universitaria Policlinico di Modena, 41124 Modena, ItalyInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Infectious Diseases, Mexico City 14080, MexicoInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Infectious Diseases, Mexico City 14080, MexicoInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Infectious Diseases, Mexico City 14080, MexicoInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Infectious Diseases, Mexico City 14080, MexicoCentro Ambulatorio para la Prevención y Atención del Sida e Infecciones de Transmisión Sexual (CAPASITS), Puebla 72410, MexicoCentro Ambulatorio para la Prevención y Atención del Sida e Infecciones de Transmisión Sexual (CAPASITS), Leon 37320, MexicoHospital General de Tijuana, Tijuana 22000, MexicoInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Infectious Diseases, Mexico City 14080, MexicoDepartment of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, 41125 Modena, ItalySeveral studies have identified main changes in T- and B-lymphocyte subsets during chronic HIV infection, but few data exist on how these subsets behave during the initial phase of HIV infection. We enrolled 22 HIV-infected patients during the acute stage of infection before the initiation of antiretroviral therapy (ART). Patients had blood samples drawn previous to ART initiation (T0), and at 2 (T1) and 12 (T2) months after ART initiation. We quantified cellular HIV-DNA content in sorted naïve and effector memory CD4 T cells and identified the main subsets of T- and B-lymphocytes using an 18-parameter flow cytometry panel. We identified correlations between the patients’ clinical and immunological data using PCA. Effective HIV treatment reduces integrated HIV DNA in effector memory T cells after 12 months (T2) of ART. The main changes in CD4+ T cells occurred at T2, with a reduction of activated memory, cytolytic and activated/exhausted stem cell memory T (T<sub>SCM</sub>) cells. Changes were present among CD8+ T cells since T1, with a reduction of several activated subsets, including activated/exhausted T<sub>SCM</sub>. At T2 a reduction of plasmablasts and exhausted B cells was also observed. A negative correlation was found between the total CD4+ T-cell count and IgM-negative plasmablasts. In patients initiating ART immediately following acute/early HIV infection, the fine analysis of T- and B-cell subsets has allowed us to identify and follow main modifications due to effective treatment, and to identify significant changes in CD4+ and CD8+ T memory stem cells.https://www.mdpi.com/2073-4409/11/15/2307HIVhighly active antiretroviral therapyT-lymphocyte subsetsB-lymphocyte subsetscellular senescencememory stem cells |
| spellingShingle | Domenico Lo Tartaro Antonio Camiro-Zúñiga Milena Nasi Sara De Biasi Marco A. Najera-Avila Maria Del Rocio Jaramillo-Jante Lara Gibellini Marcello Pinti Anita Neroni Cristina Mussini Luis E. Soto-Ramírez Juan J. Calva Francisco Belaunzarán-Zamudio Brenda Crabtree-Ramirez Christian Hernández-Leon Juan L. Mosqueda-Gómez Samuel Navarro-Álvarez Santiago Perez-Patrigeon Andrea Cossarizza Effective Treatment of Patients Experiencing Primary, Acute HIV Infection Decreases Exhausted/Activated CD4+ T Cells and CD8+ T Memory Stem Cells Cells HIV highly active antiretroviral therapy T-lymphocyte subsets B-lymphocyte subsets cellular senescence memory stem cells |
| title | Effective Treatment of Patients Experiencing Primary, Acute HIV Infection Decreases Exhausted/Activated CD4+ T Cells and CD8+ T Memory Stem Cells |
| title_full | Effective Treatment of Patients Experiencing Primary, Acute HIV Infection Decreases Exhausted/Activated CD4+ T Cells and CD8+ T Memory Stem Cells |
| title_fullStr | Effective Treatment of Patients Experiencing Primary, Acute HIV Infection Decreases Exhausted/Activated CD4+ T Cells and CD8+ T Memory Stem Cells |
| title_full_unstemmed | Effective Treatment of Patients Experiencing Primary, Acute HIV Infection Decreases Exhausted/Activated CD4+ T Cells and CD8+ T Memory Stem Cells |
| title_short | Effective Treatment of Patients Experiencing Primary, Acute HIV Infection Decreases Exhausted/Activated CD4+ T Cells and CD8+ T Memory Stem Cells |
| title_sort | effective treatment of patients experiencing primary acute hiv infection decreases exhausted activated cd4 t cells and cd8 t memory stem cells |
| topic | HIV highly active antiretroviral therapy T-lymphocyte subsets B-lymphocyte subsets cellular senescence memory stem cells |
| url | https://www.mdpi.com/2073-4409/11/15/2307 |
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