Deficiency of CCAAT/enhancer binding protein family DNA binding prevents malignant conversion of adenoma to carcinoma in NNK-induced lung carcinogenesis in the mouse
<p>Abstract</p> <p>Background</p> <p>The CCAAT/enhancer binding proteins (C/EBPs) play important roles in carcinogenesis of many tumors including the lung. Since multiple C/EBPs are expressed in lung, the combinatorial expression of these C/EBPs on lung carcinogenesis i...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-12-01
|
| Series: | Molecular Cancer |
| Subjects: | |
| Online Access: | http://www.molecular-cancer.com/content/11/1/90 |
| _version_ | 1811249475047391232 |
|---|---|
| author | Kimura Shioko Paiz Jorge Yoneda Mitsuhiro Kido Taketomo Vinson Charles Ward Jerrold M |
| author_facet | Kimura Shioko Paiz Jorge Yoneda Mitsuhiro Kido Taketomo Vinson Charles Ward Jerrold M |
| author_sort | Kimura Shioko |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The CCAAT/enhancer binding proteins (C/EBPs) play important roles in carcinogenesis of many tumors including the lung. Since multiple C/EBPs are expressed in lung, the combinatorial expression of these C/EBPs on lung carcinogenesis is not known.</p> <p>Methods</p> <p>A transgenic mouse line expressing a dominant negative A-C/EBP under the promoter of lung epithelial Clara cell secretory protein (<it>CCSP</it>) gene in doxycycline dependent fashion was subjected to 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung carcinogenesis bioassay in the presence and absence of doxycycline, and the effect of abolition of DNA binding activities of C/EBPs on lung carcinogenesis was examined.</p> <p>Results</p> <p>A-C/EBP expression was found not to interfere with tumor development; however, it suppressed the malignant conversion of adenoma to carcinoma during NNK-induced lung carcinogenesis. The results suggested that Ki67 may be used as a marker for lung carcinomas in mouse.</p> <p>Conclusions</p> <p>The DNA binding of C/EBP family members can be used as a potential molecular target for lung cancer therapy.</p> |
| first_indexed | 2024-04-12T15:48:13Z |
| format | Article |
| id | doaj.art-0f93e89eee4547aebbf90d2e5e4378f1 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-04-12T15:48:13Z |
| publishDate | 2012-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-0f93e89eee4547aebbf90d2e5e4378f12022-12-22T03:26:36ZengBMCMolecular Cancer1476-45982012-12-011119010.1186/1476-4598-11-90Deficiency of CCAAT/enhancer binding protein family DNA binding prevents malignant conversion of adenoma to carcinoma in NNK-induced lung carcinogenesis in the mouseKimura ShiokoPaiz JorgeYoneda MitsuhiroKido TaketomoVinson CharlesWard Jerrold M<p>Abstract</p> <p>Background</p> <p>The CCAAT/enhancer binding proteins (C/EBPs) play important roles in carcinogenesis of many tumors including the lung. Since multiple C/EBPs are expressed in lung, the combinatorial expression of these C/EBPs on lung carcinogenesis is not known.</p> <p>Methods</p> <p>A transgenic mouse line expressing a dominant negative A-C/EBP under the promoter of lung epithelial Clara cell secretory protein (<it>CCSP</it>) gene in doxycycline dependent fashion was subjected to 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung carcinogenesis bioassay in the presence and absence of doxycycline, and the effect of abolition of DNA binding activities of C/EBPs on lung carcinogenesis was examined.</p> <p>Results</p> <p>A-C/EBP expression was found not to interfere with tumor development; however, it suppressed the malignant conversion of adenoma to carcinoma during NNK-induced lung carcinogenesis. The results suggested that Ki67 may be used as a marker for lung carcinomas in mouse.</p> <p>Conclusions</p> <p>The DNA binding of C/EBP family members can be used as a potential molecular target for lung cancer therapy.</p>http://www.molecular-cancer.com/content/11/1/90C/EBPsLung chemical carcinogenesis bioassayDominant negativeA-C/EBPTransgenic mouse4-(methylnitrosamino)-1-(3-pyridyl)-1-butanoneNNK |
| spellingShingle | Kimura Shioko Paiz Jorge Yoneda Mitsuhiro Kido Taketomo Vinson Charles Ward Jerrold M Deficiency of CCAAT/enhancer binding protein family DNA binding prevents malignant conversion of adenoma to carcinoma in NNK-induced lung carcinogenesis in the mouse Molecular Cancer C/EBPs Lung chemical carcinogenesis bioassay Dominant negative A-C/EBP Transgenic mouse 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone NNK |
| title | Deficiency of CCAAT/enhancer binding protein family DNA binding prevents malignant conversion of adenoma to carcinoma in NNK-induced lung carcinogenesis in the mouse |
| title_full | Deficiency of CCAAT/enhancer binding protein family DNA binding prevents malignant conversion of adenoma to carcinoma in NNK-induced lung carcinogenesis in the mouse |
| title_fullStr | Deficiency of CCAAT/enhancer binding protein family DNA binding prevents malignant conversion of adenoma to carcinoma in NNK-induced lung carcinogenesis in the mouse |
| title_full_unstemmed | Deficiency of CCAAT/enhancer binding protein family DNA binding prevents malignant conversion of adenoma to carcinoma in NNK-induced lung carcinogenesis in the mouse |
| title_short | Deficiency of CCAAT/enhancer binding protein family DNA binding prevents malignant conversion of adenoma to carcinoma in NNK-induced lung carcinogenesis in the mouse |
| title_sort | deficiency of ccaat enhancer binding protein family dna binding prevents malignant conversion of adenoma to carcinoma in nnk induced lung carcinogenesis in the mouse |
| topic | C/EBPs Lung chemical carcinogenesis bioassay Dominant negative A-C/EBP Transgenic mouse 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone NNK |
| url | http://www.molecular-cancer.com/content/11/1/90 |
| work_keys_str_mv | AT kimurashioko deficiencyofccaatenhancerbindingproteinfamilydnabindingpreventsmalignantconversionofadenomatocarcinomainnnkinducedlungcarcinogenesisinthemouse AT paizjorge deficiencyofccaatenhancerbindingproteinfamilydnabindingpreventsmalignantconversionofadenomatocarcinomainnnkinducedlungcarcinogenesisinthemouse AT yonedamitsuhiro deficiencyofccaatenhancerbindingproteinfamilydnabindingpreventsmalignantconversionofadenomatocarcinomainnnkinducedlungcarcinogenesisinthemouse AT kidotaketomo deficiencyofccaatenhancerbindingproteinfamilydnabindingpreventsmalignantconversionofadenomatocarcinomainnnkinducedlungcarcinogenesisinthemouse AT vinsoncharles deficiencyofccaatenhancerbindingproteinfamilydnabindingpreventsmalignantconversionofadenomatocarcinomainnnkinducedlungcarcinogenesisinthemouse AT wardjerroldm deficiencyofccaatenhancerbindingproteinfamilydnabindingpreventsmalignantconversionofadenomatocarcinomainnnkinducedlungcarcinogenesisinthemouse |