Jorvik: A membrane-containing phage that will likely found a new family within Vinavirales
Summary: Although membrane-containing dsDNA bacterial viruses are some of the most prevalent predators in aquatic environments, we know little about how they function due to their intractability in the laboratory. Here, we have identified and thoroughly characterized a new type of membrane-containin...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223021818 |
| _version_ | 1797660087228039168 |
|---|---|
| author | Pavol Bárdy Conor I.W. MacDonald Roman Pantůček Alfred A. Antson Paul C.M. Fogg |
| author_facet | Pavol Bárdy Conor I.W. MacDonald Roman Pantůček Alfred A. Antson Paul C.M. Fogg |
| author_sort | Pavol Bárdy |
| collection | DOAJ |
| description | Summary: Although membrane-containing dsDNA bacterial viruses are some of the most prevalent predators in aquatic environments, we know little about how they function due to their intractability in the laboratory. Here, we have identified and thoroughly characterized a new type of membrane-containing bacteriophage, Jorvik, that infects the freshwater mixotrophic model bacterium Rhodobacter capsulatus. Jorvik is extremely virulent, can persist in the host integrated into the RuBisCo operon and encodes two experimentally verified cell wall hydrolases. Jorvik-like prophages are abundant in the genomes of Alphaproteobacteria, are distantly related to known viruses of the class Tectiliviricetes, and we propose they should be classified as a new family. Crucially, we demonstrate how widely used phage manipulation methods should be adjusted to prevent loss of virus infectivity. Our thorough characterization of environmental phage Jorvik provides important experimental insights about phage diversity and interactions in microbial communities that are often unexplored in common metagenomic analyses. |
| first_indexed | 2024-03-11T18:24:42Z |
| format | Article |
| id | doaj.art-0f99befcc1a54626a1670b2fb1f78963 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T18:24:42Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-0f99befcc1a54626a1670b2fb1f789632023-10-14T04:45:10ZengElsevieriScience2589-00422023-11-012611108104Jorvik: A membrane-containing phage that will likely found a new family within VinaviralesPavol Bárdy0Conor I.W. MacDonald1Roman Pantůček2Alfred A. Antson3Paul C.M. Fogg4York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UKYork Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UKDepartment of Experimental Biology, Faculty of Science, Masaryk University, 625 00 Brno, Czech RepublicYork Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK; York Biomedical Research Institute, University of York, Wentworth Way, York YO10 5NG, UKYork Biomedical Research Institute, University of York, Wentworth Way, York YO10 5NG, UK; Biology Department, University of York, Wentworth Way, York YO10 5DD, UK; Corresponding authorSummary: Although membrane-containing dsDNA bacterial viruses are some of the most prevalent predators in aquatic environments, we know little about how they function due to their intractability in the laboratory. Here, we have identified and thoroughly characterized a new type of membrane-containing bacteriophage, Jorvik, that infects the freshwater mixotrophic model bacterium Rhodobacter capsulatus. Jorvik is extremely virulent, can persist in the host integrated into the RuBisCo operon and encodes two experimentally verified cell wall hydrolases. Jorvik-like prophages are abundant in the genomes of Alphaproteobacteria, are distantly related to known viruses of the class Tectiliviricetes, and we propose they should be classified as a new family. Crucially, we demonstrate how widely used phage manipulation methods should be adjusted to prevent loss of virus infectivity. Our thorough characterization of environmental phage Jorvik provides important experimental insights about phage diversity and interactions in microbial communities that are often unexplored in common metagenomic analyses.http://www.sciencedirect.com/science/article/pii/S2589004223021818VirologyEcologyMicrobiology |
| spellingShingle | Pavol Bárdy Conor I.W. MacDonald Roman Pantůček Alfred A. Antson Paul C.M. Fogg Jorvik: A membrane-containing phage that will likely found a new family within Vinavirales iScience Virology Ecology Microbiology |
| title | Jorvik: A membrane-containing phage that will likely found a new family within Vinavirales |
| title_full | Jorvik: A membrane-containing phage that will likely found a new family within Vinavirales |
| title_fullStr | Jorvik: A membrane-containing phage that will likely found a new family within Vinavirales |
| title_full_unstemmed | Jorvik: A membrane-containing phage that will likely found a new family within Vinavirales |
| title_short | Jorvik: A membrane-containing phage that will likely found a new family within Vinavirales |
| title_sort | jorvik a membrane containing phage that will likely found a new family within vinavirales |
| topic | Virology Ecology Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223021818 |
| work_keys_str_mv | AT pavolbardy jorvikamembranecontainingphagethatwilllikelyfoundanewfamilywithinvinavirales AT conoriwmacdonald jorvikamembranecontainingphagethatwilllikelyfoundanewfamilywithinvinavirales AT romanpantucek jorvikamembranecontainingphagethatwilllikelyfoundanewfamilywithinvinavirales AT alfredaantson jorvikamembranecontainingphagethatwilllikelyfoundanewfamilywithinvinavirales AT paulcmfogg jorvikamembranecontainingphagethatwilllikelyfoundanewfamilywithinvinavirales |