Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI
Abstract SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant c...
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| Format: | Article |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-05422-w |
| _version_ | 1818845696160169984 |
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| author | Nicolás Santander Carlos Lizama María José Parga Alonso Quiroz Druso Pérez Guadalupe Echeverría Lorena Ulloa Verónica Palma Attilio Rigotti Dolores Busso |
| author_facet | Nicolás Santander Carlos Lizama María José Parga Alonso Quiroz Druso Pérez Guadalupe Echeverría Lorena Ulloa Verónica Palma Attilio Rigotti Dolores Busso |
| author_sort | Nicolás Santander |
| collection | DOAJ |
| description | Abstract SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI−/− embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic vitamin E uptake during murine neural tube closure. Our results showed that SR-BI−/− embryos had a very low vitamin E content in comparison to SR-BI+/+ embryos. Whereas SR-BI−/− embryos with closed neural tubes (nSR-BI−/−) had high levels of reactive oxygen species (ROS), intermediate ROS levels between SR-BI+/+ and nSR-BI−/− embryos were detected in SR-BI−/− with NTD (NTD SR-BI−/−). Reduced expression of Pax3, Alx1 and Alx3 genes was found in NTD SR-BI−/− embryos. Maternal α-tocopherol dietary supplementation prevented NTD almost completely (from 54% to 2%, p < 0.001) in SR-BI−/− embryos and normalized ROS and gene expression levels. In sum, our results suggest the involvement of SR-BI in the maternal provision of embryonic vitamin E to the mouse embryo during neural tube closure. |
| first_indexed | 2024-12-19T05:33:45Z |
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| id | doaj.art-0f99edc1a4cf436791774ab3c33aae83 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:33:45Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0f99edc1a4cf436791774ab3c33aae832022-12-21T20:34:10ZengNature PortfolioScientific Reports2045-23222017-07-017111110.1038/s41598-017-05422-wDeficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BINicolás Santander0Carlos Lizama1María José Parga2Alonso Quiroz3Druso Pérez4Guadalupe Echeverría5Lorena Ulloa6Verónica Palma7Attilio Rigotti8Dolores Busso9Department of Nutrition, Diabetes and Metabolism, School of Medicine, Pontificia Universidad Católica de ChileCardiovascular Research Institute, University of CaliforniaDepartment of Nutrition, Diabetes and Metabolism, School of Medicine, Pontificia Universidad Católica de ChileDepartment of Nutrition, Diabetes and Metabolism, School of Medicine, Pontificia Universidad Católica de ChileCenter of Molecular Nutrition and Chronic Diseases, School of Medicine, Pontificia Universidad Católica de ChileCenter of Molecular Nutrition and Chronic Diseases, School of Medicine, Pontificia Universidad Católica de ChileLaboratory of Stem Cell and Developmental Biology, Faculty of Sciences, Universidad de ChileLaboratory of Stem Cell and Developmental Biology, Faculty of Sciences, Universidad de ChileDepartment of Nutrition, Diabetes and Metabolism, School of Medicine, Pontificia Universidad Católica de ChileDepartment of Nutrition, Diabetes and Metabolism, School of Medicine, Pontificia Universidad Católica de ChileAbstract SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI−/− embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic vitamin E uptake during murine neural tube closure. Our results showed that SR-BI−/− embryos had a very low vitamin E content in comparison to SR-BI+/+ embryos. Whereas SR-BI−/− embryos with closed neural tubes (nSR-BI−/−) had high levels of reactive oxygen species (ROS), intermediate ROS levels between SR-BI+/+ and nSR-BI−/− embryos were detected in SR-BI−/− with NTD (NTD SR-BI−/−). Reduced expression of Pax3, Alx1 and Alx3 genes was found in NTD SR-BI−/− embryos. Maternal α-tocopherol dietary supplementation prevented NTD almost completely (from 54% to 2%, p < 0.001) in SR-BI−/− embryos and normalized ROS and gene expression levels. In sum, our results suggest the involvement of SR-BI in the maternal provision of embryonic vitamin E to the mouse embryo during neural tube closure.https://doi.org/10.1038/s41598-017-05422-w |
| spellingShingle | Nicolás Santander Carlos Lizama María José Parga Alonso Quiroz Druso Pérez Guadalupe Echeverría Lorena Ulloa Verónica Palma Attilio Rigotti Dolores Busso Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI Scientific Reports |
| title | Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI |
| title_full | Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI |
| title_fullStr | Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI |
| title_full_unstemmed | Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI |
| title_short | Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI |
| title_sort | deficient vitamin e uptake during development impairs neural tube closure in mice lacking lipoprotein receptor sr bi |
| url | https://doi.org/10.1038/s41598-017-05422-w |
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