Variation of the 2D Pattern of Brain Proteins in Mice Infected with <i>Taenia crassiceps</i> ORF Strain
Some parasites are known to influence brain proteins or induce changes in the functioning of the nervous system. In this study, our objective is to demonstrate how the two-dimensional gel technique is valuable for detecting differences in protein expression and providing detailed information on chan...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1460 |
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| author | Mariana Díaz-Zaragoza Ricardo Hernández-Ávila Abraham Landa Pedro Ostoa-Saloma |
| author_facet | Mariana Díaz-Zaragoza Ricardo Hernández-Ávila Abraham Landa Pedro Ostoa-Saloma |
| author_sort | Mariana Díaz-Zaragoza |
| collection | DOAJ |
| description | Some parasites are known to influence brain proteins or induce changes in the functioning of the nervous system. In this study, our objective is to demonstrate how the two-dimensional gel technique is valuable for detecting differences in protein expression and providing detailed information on changes in the brain proteome during a parasitic infection. Subsequently, we seek to understand how the parasitic infection affects the protein composition in the brain and how this may be related to changes in brain function. By analyzing de novo-expressed proteins at 2, 4, and 8 weeks post-infection compared to the brains of the control mice, we observed that proteins expressed at 2 weeks are primarily associated with neuroprotection or the initial response of the mouse brain to the infection. At 8 weeks, parasitic infection can induce oxidative stress in the brain, potentially activating signaling pathways related to the response to cellular damage. Proteins expressed at 8 weeks exhibit a pattern indicating that, as the host fails to balance the Neuro-Immuno-Endocrine network of the organism, the brain begins to undergo an apoptotic process and consequently experiences brain damage. |
| first_indexed | 2024-03-08T03:56:15Z |
| format | Article |
| id | doaj.art-0f9a37df8bf1461a88596964820f8863 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:56:15Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0f9a37df8bf1461a88596964820f88632024-02-09T15:13:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01253146010.3390/ijms25031460Variation of the 2D Pattern of Brain Proteins in Mice Infected with <i>Taenia crassiceps</i> ORF StrainMariana Díaz-Zaragoza0Ricardo Hernández-Ávila1Abraham Landa2Pedro Ostoa-Saloma3Departamento de Ciencias de la Salud, Centro Universitario de los Valles, Universidad de Guadalajara, Carretera Guadalajara-Ameca Km. 45.5, Guadalajara 46600, MexicoDepartamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, A.P. 70228, Mexico City 04510, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, A.P. 70228, Mexico City 04510, MexicoDepartamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, A.P. 70228, Mexico City 04510, MexicoSome parasites are known to influence brain proteins or induce changes in the functioning of the nervous system. In this study, our objective is to demonstrate how the two-dimensional gel technique is valuable for detecting differences in protein expression and providing detailed information on changes in the brain proteome during a parasitic infection. Subsequently, we seek to understand how the parasitic infection affects the protein composition in the brain and how this may be related to changes in brain function. By analyzing de novo-expressed proteins at 2, 4, and 8 weeks post-infection compared to the brains of the control mice, we observed that proteins expressed at 2 weeks are primarily associated with neuroprotection or the initial response of the mouse brain to the infection. At 8 weeks, parasitic infection can induce oxidative stress in the brain, potentially activating signaling pathways related to the response to cellular damage. Proteins expressed at 8 weeks exhibit a pattern indicating that, as the host fails to balance the Neuro-Immuno-Endocrine network of the organism, the brain begins to undergo an apoptotic process and consequently experiences brain damage.https://www.mdpi.com/1422-0067/25/3/1460<i>Taenia crassiceps</i>brain damagebrain proteomeNeuro-Immuno-Endocrine network |
| spellingShingle | Mariana Díaz-Zaragoza Ricardo Hernández-Ávila Abraham Landa Pedro Ostoa-Saloma Variation of the 2D Pattern of Brain Proteins in Mice Infected with <i>Taenia crassiceps</i> ORF Strain International Journal of Molecular Sciences <i>Taenia crassiceps</i> brain damage brain proteome Neuro-Immuno-Endocrine network |
| title | Variation of the 2D Pattern of Brain Proteins in Mice Infected with <i>Taenia crassiceps</i> ORF Strain |
| title_full | Variation of the 2D Pattern of Brain Proteins in Mice Infected with <i>Taenia crassiceps</i> ORF Strain |
| title_fullStr | Variation of the 2D Pattern of Brain Proteins in Mice Infected with <i>Taenia crassiceps</i> ORF Strain |
| title_full_unstemmed | Variation of the 2D Pattern of Brain Proteins in Mice Infected with <i>Taenia crassiceps</i> ORF Strain |
| title_short | Variation of the 2D Pattern of Brain Proteins in Mice Infected with <i>Taenia crassiceps</i> ORF Strain |
| title_sort | variation of the 2d pattern of brain proteins in mice infected with i taenia crassiceps i orf strain |
| topic | <i>Taenia crassiceps</i> brain damage brain proteome Neuro-Immuno-Endocrine network |
| url | https://www.mdpi.com/1422-0067/25/3/1460 |
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