Plasma Extracellular Vesicle miRNA Profiles Distinguish Chronic Obstructive Pulmonary Disease Exacerbations and Disease Severity
Hannah E O’Farrell, Rayleen V Bowman, Kwun M Fong, Ian A Yang Faculty of Medicine, the University of Queensland, Brisbane, QLD, AustraliaCorrespondence: Hannah E O’Farrell, Faculty of Medicine, the University of Queensland, Brisbane, QLD, Australia, Tel +61 07 3139 4110, Email hannah.ofarrell@uq.net...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-11-01
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| Series: | International Journal of COPD |
| Subjects: | |
| Online Access: | https://www.dovepress.com/plasma-extracellular-vesicle-mirna-profiles-distinguish-chronic-obstru-peer-reviewed-fulltext-article-COPD |
| _version_ | 1828094965795782656 |
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| author | O'Farrell HE Bowman RV Fong KM Yang IA |
| author_facet | O'Farrell HE Bowman RV Fong KM Yang IA |
| author_sort | O'Farrell HE |
| collection | DOAJ |
| description | Hannah E O’Farrell, Rayleen V Bowman, Kwun M Fong, Ian A Yang Faculty of Medicine, the University of Queensland, Brisbane, QLD, AustraliaCorrespondence: Hannah E O’Farrell, Faculty of Medicine, the University of Queensland, Brisbane, QLD, Australia, Tel +61 07 3139 4110, Email hannah.ofarrell@uq.net.auPurpose: Molecular biomarkers for chronic obstructive pulmonary disease (COPD) severity have been difficult to identify. We aimed to assess extracellular vesicle miRNAs’ potential as a blood biomarker in discriminating disease severity in participants with COPD.Patients and Methods: Plasma extracellular vesicles (EVs) were obtained from two COPD cohorts (n = 20 during an exacerbation event, n = 20 during stable state), with varying disease severity (GOLD stages). The miRCURY LNA miRNA Serum/Plasma assay, specific to 179 targets, was used to evaluate EV miRNA expression. The miRNAs that were significantly dysregulated were further assessed for discriminatory power using ROC curve analysis, as well as their role in relevant biological pathways.Results: One miRNA was significantly dysregulated between moderate GOLD participants compared to severe/very severe GOLD participants, with an AUC of 0.798, p = 0.01 for miR-374b-5p. Five miRNAs were significantly dysregulated between exacerbating and stable COPD participants, with miR-223-3p resulting in the highest AUC (0.755, p = 0.006) for a single miRNA, with a combination of three miRNAs (miR-92b-3p, miR-374a-5p and miR-106b-3p) providing the highest discriminatory power (AUC 0.820, p = 0.001). The “cytokine–cytokine receptor interaction” (hsa04060 pathway) was the most significant KEGG pathway enriched for three out of the five miRNAs associated with COPD exacerbations.Conclusion: This initial small-scale study suggests that the bioactive cargo (miRNAs) in plasma EVs holds specific biological information for the severity of airflow obstruction and COPD exacerbations, warranting further investigation.Keywords: COPD, exacerbations, extracellular vesicles, miRNAs |
| first_indexed | 2024-04-11T07:06:23Z |
| format | Article |
| id | doaj.art-0f9d9f1852fd41ada1fa99760862c006 |
| institution | Directory Open Access Journal |
| issn | 1178-2005 |
| language | English |
| last_indexed | 2024-04-11T07:06:23Z |
| publishDate | 2022-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of COPD |
| spelling | doaj.art-0f9d9f1852fd41ada1fa99760862c0062022-12-22T04:38:23ZengDove Medical PressInternational Journal of COPD1178-20052022-11-01Volume 172821283379438Plasma Extracellular Vesicle miRNA Profiles Distinguish Chronic Obstructive Pulmonary Disease Exacerbations and Disease SeverityO'Farrell HEBowman RVFong KMYang IAHannah E O’Farrell, Rayleen V Bowman, Kwun M Fong, Ian A Yang Faculty of Medicine, the University of Queensland, Brisbane, QLD, AustraliaCorrespondence: Hannah E O’Farrell, Faculty of Medicine, the University of Queensland, Brisbane, QLD, Australia, Tel +61 07 3139 4110, Email hannah.ofarrell@uq.net.auPurpose: Molecular biomarkers for chronic obstructive pulmonary disease (COPD) severity have been difficult to identify. We aimed to assess extracellular vesicle miRNAs’ potential as a blood biomarker in discriminating disease severity in participants with COPD.Patients and Methods: Plasma extracellular vesicles (EVs) were obtained from two COPD cohorts (n = 20 during an exacerbation event, n = 20 during stable state), with varying disease severity (GOLD stages). The miRCURY LNA miRNA Serum/Plasma assay, specific to 179 targets, was used to evaluate EV miRNA expression. The miRNAs that were significantly dysregulated were further assessed for discriminatory power using ROC curve analysis, as well as their role in relevant biological pathways.Results: One miRNA was significantly dysregulated between moderate GOLD participants compared to severe/very severe GOLD participants, with an AUC of 0.798, p = 0.01 for miR-374b-5p. Five miRNAs were significantly dysregulated between exacerbating and stable COPD participants, with miR-223-3p resulting in the highest AUC (0.755, p = 0.006) for a single miRNA, with a combination of three miRNAs (miR-92b-3p, miR-374a-5p and miR-106b-3p) providing the highest discriminatory power (AUC 0.820, p = 0.001). The “cytokine–cytokine receptor interaction” (hsa04060 pathway) was the most significant KEGG pathway enriched for three out of the five miRNAs associated with COPD exacerbations.Conclusion: This initial small-scale study suggests that the bioactive cargo (miRNAs) in plasma EVs holds specific biological information for the severity of airflow obstruction and COPD exacerbations, warranting further investigation.Keywords: COPD, exacerbations, extracellular vesicles, miRNAshttps://www.dovepress.com/plasma-extracellular-vesicle-mirna-profiles-distinguish-chronic-obstru-peer-reviewed-fulltext-article-COPDcopdexacerbationsextracellular vesiclesmirnas |
| spellingShingle | O'Farrell HE Bowman RV Fong KM Yang IA Plasma Extracellular Vesicle miRNA Profiles Distinguish Chronic Obstructive Pulmonary Disease Exacerbations and Disease Severity International Journal of COPD copd exacerbations extracellular vesicles mirnas |
| title | Plasma Extracellular Vesicle miRNA Profiles Distinguish Chronic Obstructive Pulmonary Disease Exacerbations and Disease Severity |
| title_full | Plasma Extracellular Vesicle miRNA Profiles Distinguish Chronic Obstructive Pulmonary Disease Exacerbations and Disease Severity |
| title_fullStr | Plasma Extracellular Vesicle miRNA Profiles Distinguish Chronic Obstructive Pulmonary Disease Exacerbations and Disease Severity |
| title_full_unstemmed | Plasma Extracellular Vesicle miRNA Profiles Distinguish Chronic Obstructive Pulmonary Disease Exacerbations and Disease Severity |
| title_short | Plasma Extracellular Vesicle miRNA Profiles Distinguish Chronic Obstructive Pulmonary Disease Exacerbations and Disease Severity |
| title_sort | plasma extracellular vesicle mirna profiles distinguish chronic obstructive pulmonary disease exacerbations and disease severity |
| topic | copd exacerbations extracellular vesicles mirnas |
| url | https://www.dovepress.com/plasma-extracellular-vesicle-mirna-profiles-distinguish-chronic-obstru-peer-reviewed-fulltext-article-COPD |
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