Effect of photoinitiator on chain degradation of hyaluronic acid
Abstract Objectives Photocrosslinking systems of polymers have been widely studied using UV or visible light irradiation. However, the photodegradation behavior derived from light irradiation was rarely reported, comparing with the photocrosslinking. In this study, the tyramine-modified hyaluronic a...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2019-11-01
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| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40824-019-0170-1 |
| _version_ | 1827333597356359680 |
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| author | Bo Min Hong Su A Park Won Ho Park |
| author_facet | Bo Min Hong Su A Park Won Ho Park |
| author_sort | Bo Min Hong |
| collection | DOAJ |
| description | Abstract Objectives Photocrosslinking systems of polymers have been widely studied using UV or visible light irradiation. However, the photodegradation behavior derived from light irradiation was rarely reported, comparing with the photocrosslinking. In this study, the tyramine-modified hyaluronic acid (HA/Tyr) hydrogel was prepared using riboflavin (RF) as a photoinitiator, and the degradation behavior of HA by the reactive oxygen species (ROS) generated in photochemical process was investigated. Materials and methods The HA/Tyr conjugate was synthesized by EDC/NHS chemistry to introduce phenol group. Degree of substitution (DS, %) of phenol group to HA molecule was about 25%. The structural change of HA/Tyr was measured by proton nuclear magnetic resonance (1H-NMR) and attenuated total reflectance infrared spectroscopy (ATR-FTIR), and the rheological properties of photocrosslinked HA/Tyr hydrogel were investigated by rheometer. Results The HA/Tyr solution with 25% substitution formed a stable hydrogel via visible light irradiation in the presence of RF photoinitiator. Rheological data of HA/Tyr solution showed that the storage modulus (G’) was increased with increasing HA concentration. Additionally, it was found that RF initiated by visible light irradiation induced the degradation of HA molecular chain, and consequently reduced the viscosity of HA/Tyr solutions. Conclusion The results indicate that RF-based photoinitiator system caused the degradation of HA molecule by ROS generated in photochemical process as well as the crosslinking of HA/Tyr. |
| first_indexed | 2024-03-07T17:29:44Z |
| format | Article |
| id | doaj.art-0f9fe95111184e8ba580806e46480a20 |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T17:29:44Z |
| publishDate | 2019-11-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-0f9fe95111184e8ba580806e46480a202024-03-02T18:11:35ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242019-11-012311810.1186/s40824-019-0170-1Effect of photoinitiator on chain degradation of hyaluronic acidBo Min Hong0Su A Park1Won Ho Park2Department of Advanced Organic Materials and Textile System Engineering, College of Engineering, Chungnam National UniversityDepartment of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and MaterialsDepartment of Advanced Organic Materials and Textile System Engineering, College of Engineering, Chungnam National UniversityAbstract Objectives Photocrosslinking systems of polymers have been widely studied using UV or visible light irradiation. However, the photodegradation behavior derived from light irradiation was rarely reported, comparing with the photocrosslinking. In this study, the tyramine-modified hyaluronic acid (HA/Tyr) hydrogel was prepared using riboflavin (RF) as a photoinitiator, and the degradation behavior of HA by the reactive oxygen species (ROS) generated in photochemical process was investigated. Materials and methods The HA/Tyr conjugate was synthesized by EDC/NHS chemistry to introduce phenol group. Degree of substitution (DS, %) of phenol group to HA molecule was about 25%. The structural change of HA/Tyr was measured by proton nuclear magnetic resonance (1H-NMR) and attenuated total reflectance infrared spectroscopy (ATR-FTIR), and the rheological properties of photocrosslinked HA/Tyr hydrogel were investigated by rheometer. Results The HA/Tyr solution with 25% substitution formed a stable hydrogel via visible light irradiation in the presence of RF photoinitiator. Rheological data of HA/Tyr solution showed that the storage modulus (G’) was increased with increasing HA concentration. Additionally, it was found that RF initiated by visible light irradiation induced the degradation of HA molecular chain, and consequently reduced the viscosity of HA/Tyr solutions. Conclusion The results indicate that RF-based photoinitiator system caused the degradation of HA molecule by ROS generated in photochemical process as well as the crosslinking of HA/Tyr.http://link.springer.com/article/10.1186/s40824-019-0170-1RiboflavinHyaluronic acidTyramineHydrogelPhotocrosslinkingDegradation |
| spellingShingle | Bo Min Hong Su A Park Won Ho Park Effect of photoinitiator on chain degradation of hyaluronic acid Biomaterials Research Riboflavin Hyaluronic acid Tyramine Hydrogel Photocrosslinking Degradation |
| title | Effect of photoinitiator on chain degradation of hyaluronic acid |
| title_full | Effect of photoinitiator on chain degradation of hyaluronic acid |
| title_fullStr | Effect of photoinitiator on chain degradation of hyaluronic acid |
| title_full_unstemmed | Effect of photoinitiator on chain degradation of hyaluronic acid |
| title_short | Effect of photoinitiator on chain degradation of hyaluronic acid |
| title_sort | effect of photoinitiator on chain degradation of hyaluronic acid |
| topic | Riboflavin Hyaluronic acid Tyramine Hydrogel Photocrosslinking Degradation |
| url | http://link.springer.com/article/10.1186/s40824-019-0170-1 |
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