New record of the Oriental house rat, Rattus tanezumi, in Nepal inferred from mitochondrial Cytochrome B gene sequences
This study determines the presence of R. tanezumi from in Nepal using morphological and molecular analyses. Morphologically, it is indistinguishable with R. rattus owing to similar fur colour and morphometric data. However, molecular identification and phylogenetic analysis using sequences of the mi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Mitochondrial DNA. Part B. Resources |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23802359.2018.1436991 |
| _version_ | 1797640732331212800 |
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| author | Pradeep Adhikari Sang-Hyun Han Yoo-Kyung Kim Tae-Wook Kim Tej Bahadur Thapa Naresh Subedi Amar Kunwar Maniram Banjade Hong-Shik Oh |
| author_facet | Pradeep Adhikari Sang-Hyun Han Yoo-Kyung Kim Tae-Wook Kim Tej Bahadur Thapa Naresh Subedi Amar Kunwar Maniram Banjade Hong-Shik Oh |
| author_sort | Pradeep Adhikari |
| collection | DOAJ |
| description | This study determines the presence of R. tanezumi from in Nepal using morphological and molecular analyses. Morphologically, it is indistinguishable with R. rattus owing to similar fur colour and morphometric data. However, molecular identification and phylogenetic analysis using sequences of the mitochondrial DNA (mtDNA) Cytochrome B (CytB) gene revealed two different species R. rattus and R. tanezumi from collected specimens. The genetic distance between R. rattus and R. tanezumi was found 0.043. In phylogenetic tree, the clade of R. tanezumi is distinguished into two sub-clades, R. tanezumi found in Nepal, and East Asian countries, China, Laos, Thailand, Viet Nam, and South Korea have genetic distance 0.031, suggesting the different lineages of R. tanezumi. This study confirmed the R. tanezumi present in Nepal. Our findings suggest that morphological analysis and molecular study should be carried out simultaneously for accurate identification of small sized cryptic mammals like R. tanezumi and R. rattus. |
| first_indexed | 2024-03-11T13:35:03Z |
| format | Article |
| id | doaj.art-0fa1638036d241c1a9bd471477685bca |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:35:03Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-0fa1638036d241c1a9bd471477685bca2023-11-02T15:43:00ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592018-01-013138639010.1080/23802359.2018.14369911436991New record of the Oriental house rat, Rattus tanezumi, in Nepal inferred from mitochondrial Cytochrome B gene sequencesPradeep Adhikari0Sang-Hyun Han1Yoo-Kyung Kim2Tae-Wook Kim3Tej Bahadur Thapa4Naresh Subedi5Amar Kunwar6Maniram Banjade7Hong-Shik Oh8Jeju National UniversityEducational Science Research Institute, Jeju National UniversityEducational Science Research Institute, Jeju National UniversitySpecies Restoration Technology Institute, Korea National Park ServiceTribhuvan UniversityNational Trust for Nature ConservationSmall Mammals Conservation and Research FoundationJeju National UniversityJeju National UniversityThis study determines the presence of R. tanezumi from in Nepal using morphological and molecular analyses. Morphologically, it is indistinguishable with R. rattus owing to similar fur colour and morphometric data. However, molecular identification and phylogenetic analysis using sequences of the mitochondrial DNA (mtDNA) Cytochrome B (CytB) gene revealed two different species R. rattus and R. tanezumi from collected specimens. The genetic distance between R. rattus and R. tanezumi was found 0.043. In phylogenetic tree, the clade of R. tanezumi is distinguished into two sub-clades, R. tanezumi found in Nepal, and East Asian countries, China, Laos, Thailand, Viet Nam, and South Korea have genetic distance 0.031, suggesting the different lineages of R. tanezumi. This study confirmed the R. tanezumi present in Nepal. Our findings suggest that morphological analysis and molecular study should be carried out simultaneously for accurate identification of small sized cryptic mammals like R. tanezumi and R. rattus.http://dx.doi.org/10.1080/23802359.2018.1436991cytbmorphological charactersnepalr. rattusr. tanezumi |
| spellingShingle | Pradeep Adhikari Sang-Hyun Han Yoo-Kyung Kim Tae-Wook Kim Tej Bahadur Thapa Naresh Subedi Amar Kunwar Maniram Banjade Hong-Shik Oh New record of the Oriental house rat, Rattus tanezumi, in Nepal inferred from mitochondrial Cytochrome B gene sequences Mitochondrial DNA. Part B. Resources cytb morphological characters nepal r. rattus r. tanezumi |
| title | New record of the Oriental house rat, Rattus tanezumi, in Nepal inferred from mitochondrial Cytochrome B gene sequences |
| title_full | New record of the Oriental house rat, Rattus tanezumi, in Nepal inferred from mitochondrial Cytochrome B gene sequences |
| title_fullStr | New record of the Oriental house rat, Rattus tanezumi, in Nepal inferred from mitochondrial Cytochrome B gene sequences |
| title_full_unstemmed | New record of the Oriental house rat, Rattus tanezumi, in Nepal inferred from mitochondrial Cytochrome B gene sequences |
| title_short | New record of the Oriental house rat, Rattus tanezumi, in Nepal inferred from mitochondrial Cytochrome B gene sequences |
| title_sort | new record of the oriental house rat rattus tanezumi in nepal inferred from mitochondrial cytochrome b gene sequences |
| topic | cytb morphological characters nepal r. rattus r. tanezumi |
| url | http://dx.doi.org/10.1080/23802359.2018.1436991 |
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