The Predictive Value of Graft Viability and Bioenergetics Testing Towards the Outcome in Liver Transplantation
Donor organ biomarkers with sufficient predictive value in liver transplantation (LT) are lacking. We herein evaluate liver viability and mitochondrial bioenergetics for their predictive capacity towards the outcome in LT. We enrolled 43 consecutive patients undergoing LT. Liver biopsy samples taken...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Transplant International |
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| Online Access: | https://www.frontierspartnerships.org/articles/10.3389/ti.2024.12380/full |
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| author | Andras T. Meszaros Annemarie Weissenbacher Melanie Schartner Tim Egelseer-Bruendl Martin Hermann Jasmin Unterweger Christa Mittelberger Beatrix A. Reyer Julia Hofmann Bettina G. Zelger Theresa Hautz Thomas Resch Christian Margreiter Manuel Maglione Timea Komlódi Hanno Ulmer Benno Cardini Jakob Troppmair Dietmar Öfner Erich Gnaiger Stefan Schneeberger Rupert Oberhuber |
| author_facet | Andras T. Meszaros Annemarie Weissenbacher Melanie Schartner Tim Egelseer-Bruendl Martin Hermann Jasmin Unterweger Christa Mittelberger Beatrix A. Reyer Julia Hofmann Bettina G. Zelger Theresa Hautz Thomas Resch Christian Margreiter Manuel Maglione Timea Komlódi Hanno Ulmer Benno Cardini Jakob Troppmair Dietmar Öfner Erich Gnaiger Stefan Schneeberger Rupert Oberhuber |
| author_sort | Andras T. Meszaros |
| collection | DOAJ |
| description | Donor organ biomarkers with sufficient predictive value in liver transplantation (LT) are lacking. We herein evaluate liver viability and mitochondrial bioenergetics for their predictive capacity towards the outcome in LT. We enrolled 43 consecutive patients undergoing LT. Liver biopsy samples taken upon arrival after static cold storage were assessed by histology, real-time confocal imaging analysis (RTCA), and high-resolution respirometry (HRR) for mitochondrial respiration of tissue homogenates. Early allograft dysfunction (EAD) served as primary endpoint. HRR data were analysed with a focus on the efficacy of ATP production or P-L control efficiency, calculated as 1-L/P from the capacity of oxidative phosphorylation P and non-phosphorylating respiration L. Twenty-two recipients experienced EAD. Pre-transplant histology was not predictive of EAD. The mean RTCA score was significantly lower in the EAD cohort (−0.75 ± 2.27) compared to the IF cohort (0.70 ± 2.08; p = 0.01), indicating decreased cell viability. P-L control efficiency was predictive of EAD (0.76 ± 0.06 in IF vs. 0.70 ± 0.08 in EAD-livers; p = 0.02) and correlated with the RTCA score. Both RTCA and P-L control efficiency in biopsy samples taken during cold storage have predictive capacity towards the outcome in LT. Therefore, RTCA and HRR should be considered for risk stratification, viability assessment, and bioenergetic testing in liver transplantation. |
| first_indexed | 2024-04-24T13:18:57Z |
| format | Article |
| id | doaj.art-0fa1744622fe42b993bcf6d4d4930586 |
| institution | Directory Open Access Journal |
| issn | 1432-2277 |
| language | English |
| last_indexed | 2024-04-24T13:18:57Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Transplant International |
| spelling | doaj.art-0fa1744622fe42b993bcf6d4d49305862024-04-04T16:20:49ZengFrontiers Media S.A.Transplant International1432-22772024-02-013710.3389/ti.2024.1238012380The Predictive Value of Graft Viability and Bioenergetics Testing Towards the Outcome in Liver TransplantationAndras T. Meszaros0Annemarie Weissenbacher1Melanie Schartner2Tim Egelseer-Bruendl3Martin Hermann4Jasmin Unterweger5Christa Mittelberger6Beatrix A. Reyer7Julia Hofmann8Bettina G. Zelger9Theresa Hautz10Thomas Resch11Christian Margreiter12Manuel Maglione13Timea Komlódi14Hanno Ulmer15Benno Cardini16Jakob Troppmair17Dietmar Öfner18Erich Gnaiger19Stefan Schneeberger20Rupert Oberhuber21Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaInstitute of Pathology, Neuropathology and Molecular Pathology, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaOroboros Instruments, Innsbruck, AustriaDepartment of Medical Statistics, Informatics and Health Economics, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaOroboros Instruments, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Innsbruck, AustriaDonor organ biomarkers with sufficient predictive value in liver transplantation (LT) are lacking. We herein evaluate liver viability and mitochondrial bioenergetics for their predictive capacity towards the outcome in LT. We enrolled 43 consecutive patients undergoing LT. Liver biopsy samples taken upon arrival after static cold storage were assessed by histology, real-time confocal imaging analysis (RTCA), and high-resolution respirometry (HRR) for mitochondrial respiration of tissue homogenates. Early allograft dysfunction (EAD) served as primary endpoint. HRR data were analysed with a focus on the efficacy of ATP production or P-L control efficiency, calculated as 1-L/P from the capacity of oxidative phosphorylation P and non-phosphorylating respiration L. Twenty-two recipients experienced EAD. Pre-transplant histology was not predictive of EAD. The mean RTCA score was significantly lower in the EAD cohort (−0.75 ± 2.27) compared to the IF cohort (0.70 ± 2.08; p = 0.01), indicating decreased cell viability. P-L control efficiency was predictive of EAD (0.76 ± 0.06 in IF vs. 0.70 ± 0.08 in EAD-livers; p = 0.02) and correlated with the RTCA score. Both RTCA and P-L control efficiency in biopsy samples taken during cold storage have predictive capacity towards the outcome in LT. Therefore, RTCA and HRR should be considered for risk stratification, viability assessment, and bioenergetic testing in liver transplantation.https://www.frontierspartnerships.org/articles/10.3389/ti.2024.12380/fulllivertransplantationstatic cold storagemitochondriahigh-resolution respirometryreal-time confocal imaging |
| spellingShingle | Andras T. Meszaros Annemarie Weissenbacher Melanie Schartner Tim Egelseer-Bruendl Martin Hermann Jasmin Unterweger Christa Mittelberger Beatrix A. Reyer Julia Hofmann Bettina G. Zelger Theresa Hautz Thomas Resch Christian Margreiter Manuel Maglione Timea Komlódi Hanno Ulmer Benno Cardini Jakob Troppmair Dietmar Öfner Erich Gnaiger Stefan Schneeberger Rupert Oberhuber The Predictive Value of Graft Viability and Bioenergetics Testing Towards the Outcome in Liver Transplantation Transplant International liver transplantation static cold storage mitochondria high-resolution respirometry real-time confocal imaging |
| title | The Predictive Value of Graft Viability and Bioenergetics Testing Towards the Outcome in Liver Transplantation |
| title_full | The Predictive Value of Graft Viability and Bioenergetics Testing Towards the Outcome in Liver Transplantation |
| title_fullStr | The Predictive Value of Graft Viability and Bioenergetics Testing Towards the Outcome in Liver Transplantation |
| title_full_unstemmed | The Predictive Value of Graft Viability and Bioenergetics Testing Towards the Outcome in Liver Transplantation |
| title_short | The Predictive Value of Graft Viability and Bioenergetics Testing Towards the Outcome in Liver Transplantation |
| title_sort | predictive value of graft viability and bioenergetics testing towards the outcome in liver transplantation |
| topic | liver transplantation static cold storage mitochondria high-resolution respirometry real-time confocal imaging |
| url | https://www.frontierspartnerships.org/articles/10.3389/ti.2024.12380/full |
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