Acetylcholinesterase Immobilization on ZIF-8/Graphene Composite Engenders High Sensitivity Electrochemical Sensing for Organophosphorus Pesticides
A sensitive and flexible detection method for organophosphorus pesticides (OPs) detection is a crucial request to avoid their further expanded pollution. Herein, an acetylcholinesterase (AChE) electrochemical sensor, based on the co-modification of ZIF-8 and graphene (GR), was constructed for the de...
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MDPI AG
2022-10-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/10/10/418 |
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| author | Long Wen Ning Wang Zhuoliang Liu Cheng-an Tao Xiaorong Zou Fang Wang Jianfang Wang |
| author_facet | Long Wen Ning Wang Zhuoliang Liu Cheng-an Tao Xiaorong Zou Fang Wang Jianfang Wang |
| author_sort | Long Wen |
| collection | DOAJ |
| description | A sensitive and flexible detection method for organophosphorus pesticides (OPs) detection is a crucial request to avoid their further expanded pollution. Herein, an acetylcholinesterase (AChE) electrochemical sensor, based on the co-modification of ZIF-8 and graphene (GR), was constructed for the detection of OPs. ZIF-8/GR composite can provide a stable and biocompatible environment for the loading of AChE and can accelerate the chemical reaction on the electrode surface. After optimization, the linear detection range of the constructed AChE-CS/GR/ZIF-8/GCE sensor for ICP was 0.5–100 ng/mL (1.73–345.7 nM), and the limit of detection was 0.18 ng/mL (0.62 nM). Moreover, high sensitivity and high specificity of the sensor were also achieved in actual cabbage and tap water samples. Therefore, it has great potential for the application of organophosphorus pesticide residue analysis. |
| first_indexed | 2024-03-09T20:27:50Z |
| format | Article |
| id | doaj.art-0fa283e02e7c4e68a01cdc8b24c57209 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T20:27:50Z |
| publishDate | 2022-10-01 |
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| series | Chemosensors |
| spelling | doaj.art-0fa283e02e7c4e68a01cdc8b24c572092023-11-23T23:30:21ZengMDPI AGChemosensors2227-90402022-10-01101041810.3390/chemosensors10100418Acetylcholinesterase Immobilization on ZIF-8/Graphene Composite Engenders High Sensitivity Electrochemical Sensing for Organophosphorus PesticidesLong Wen0Ning Wang1Zhuoliang Liu2Cheng-an Tao3Xiaorong Zou4Fang Wang5Jianfang Wang6College of Science, National University of Defense Technology, Changsha 410073, ChinaCollege of Science, National University of Defense Technology, Changsha 410073, ChinaCollege of Science, National University of Defense Technology, Changsha 410073, ChinaCollege of Science, National University of Defense Technology, Changsha 410073, ChinaCollege of Science, National University of Defense Technology, Changsha 410073, ChinaCollege of Science, National University of Defense Technology, Changsha 410073, ChinaCollege of Science, National University of Defense Technology, Changsha 410073, ChinaA sensitive and flexible detection method for organophosphorus pesticides (OPs) detection is a crucial request to avoid their further expanded pollution. Herein, an acetylcholinesterase (AChE) electrochemical sensor, based on the co-modification of ZIF-8 and graphene (GR), was constructed for the detection of OPs. ZIF-8/GR composite can provide a stable and biocompatible environment for the loading of AChE and can accelerate the chemical reaction on the electrode surface. After optimization, the linear detection range of the constructed AChE-CS/GR/ZIF-8/GCE sensor for ICP was 0.5–100 ng/mL (1.73–345.7 nM), and the limit of detection was 0.18 ng/mL (0.62 nM). Moreover, high sensitivity and high specificity of the sensor were also achieved in actual cabbage and tap water samples. Therefore, it has great potential for the application of organophosphorus pesticide residue analysis.https://www.mdpi.com/2227-9040/10/10/418electrochemical biosensormetal-organic frameworkgrapheneacetylcholinesteraseisocarbophos |
| spellingShingle | Long Wen Ning Wang Zhuoliang Liu Cheng-an Tao Xiaorong Zou Fang Wang Jianfang Wang Acetylcholinesterase Immobilization on ZIF-8/Graphene Composite Engenders High Sensitivity Electrochemical Sensing for Organophosphorus Pesticides Chemosensors electrochemical biosensor metal-organic framework graphene acetylcholinesterase isocarbophos |
| title | Acetylcholinesterase Immobilization on ZIF-8/Graphene Composite Engenders High Sensitivity Electrochemical Sensing for Organophosphorus Pesticides |
| title_full | Acetylcholinesterase Immobilization on ZIF-8/Graphene Composite Engenders High Sensitivity Electrochemical Sensing for Organophosphorus Pesticides |
| title_fullStr | Acetylcholinesterase Immobilization on ZIF-8/Graphene Composite Engenders High Sensitivity Electrochemical Sensing for Organophosphorus Pesticides |
| title_full_unstemmed | Acetylcholinesterase Immobilization on ZIF-8/Graphene Composite Engenders High Sensitivity Electrochemical Sensing for Organophosphorus Pesticides |
| title_short | Acetylcholinesterase Immobilization on ZIF-8/Graphene Composite Engenders High Sensitivity Electrochemical Sensing for Organophosphorus Pesticides |
| title_sort | acetylcholinesterase immobilization on zif 8 graphene composite engenders high sensitivity electrochemical sensing for organophosphorus pesticides |
| topic | electrochemical biosensor metal-organic framework graphene acetylcholinesterase isocarbophos |
| url | https://www.mdpi.com/2227-9040/10/10/418 |
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