Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency
This article contains data related to the inflammatory cytokine and investigated pathways involved in bacterial clearance reported in “Airway infection with Nontypeable Haemophilus influenzae is more rapidly eradicated in vitamin D deficient mice” (Serré et al., 2018) [1]. Vitamin D deficient or suf...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-02-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340918315877 |
| _version_ | 1818144312836227072 |
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| author | Jef Serré Carolien Mathyssen Tom Tanjeko Ajime Hannelie Korf Karen Maes Nele Heulens Conny Gysemans Chantal Mathieu Bart Vanaudenaerde Wim Janssens Ghislaine Gayan-Ramirez |
| author_facet | Jef Serré Carolien Mathyssen Tom Tanjeko Ajime Hannelie Korf Karen Maes Nele Heulens Conny Gysemans Chantal Mathieu Bart Vanaudenaerde Wim Janssens Ghislaine Gayan-Ramirez |
| author_sort | Jef Serré |
| collection | DOAJ |
| description | This article contains data related to the inflammatory cytokine and investigated pathways involved in bacterial clearance reported in “Airway infection with Nontypeable Haemophilus influenzae is more rapidly eradicated in vitamin D deficient mice” (Serré et al., 2018) [1]. Vitamin D deficient or sufficient mice were oropharyngeally instilled with 106 NTHi and sacrificed at 4, 8, 24 and 72 h post-infection. We measured proinflammatory cytokines (KC, TNF-α, IL-1β, IL6 and MCP-1) markers of bacterial clearance pathways (myeloid peroxidase, nitric oxide, complement C5a and immunoglobulin A) in bronchoalveolar fluid (BALF) during infection and mRNA expression levels of innate immune defense mechanism markers (mucin glycoproteins, pathogen recognitions receptor TLR2 and TLR4, antimicrobial peptides SLPI, REG3γ, lysozyme, BD-1, BD-2, BD-3 and surfactant proteins SP-A and SP-D) in lung homogenate. Finally, genomic DNA of NTHi (protein D) measured in lung homogenate was used as an indicator of NTHi invasion of alveolar macrophages or epithelial cells. |
| first_indexed | 2024-12-11T11:45:34Z |
| format | Article |
| id | doaj.art-0fa49c91356f4c409679a5a379df10ca |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-11T11:45:34Z |
| publishDate | 2019-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-0fa49c91356f4c409679a5a379df10ca2022-12-22T01:08:29ZengElsevierData in Brief2352-34092019-02-0122703708Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiencyJef Serré0Carolien Mathyssen1Tom Tanjeko Ajime2Hannelie Korf3Karen Maes4Nele Heulens5Conny Gysemans6Chantal Mathieu7Bart Vanaudenaerde8Wim Janssens9Ghislaine Gayan-Ramirez10Laboratory of Respiratory Diseases, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumLaboratory of Hepatology, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumClinical and Experimental Endocrinology, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumClinical and Experimental Endocrinology, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, BelgiumLaboratory of Respiratory Diseases, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, Belgium; Corresponding author.This article contains data related to the inflammatory cytokine and investigated pathways involved in bacterial clearance reported in “Airway infection with Nontypeable Haemophilus influenzae is more rapidly eradicated in vitamin D deficient mice” (Serré et al., 2018) [1]. Vitamin D deficient or sufficient mice were oropharyngeally instilled with 106 NTHi and sacrificed at 4, 8, 24 and 72 h post-infection. We measured proinflammatory cytokines (KC, TNF-α, IL-1β, IL6 and MCP-1) markers of bacterial clearance pathways (myeloid peroxidase, nitric oxide, complement C5a and immunoglobulin A) in bronchoalveolar fluid (BALF) during infection and mRNA expression levels of innate immune defense mechanism markers (mucin glycoproteins, pathogen recognitions receptor TLR2 and TLR4, antimicrobial peptides SLPI, REG3γ, lysozyme, BD-1, BD-2, BD-3 and surfactant proteins SP-A and SP-D) in lung homogenate. Finally, genomic DNA of NTHi (protein D) measured in lung homogenate was used as an indicator of NTHi invasion of alveolar macrophages or epithelial cells.http://www.sciencedirect.com/science/article/pii/S2352340918315877 |
| spellingShingle | Jef Serré Carolien Mathyssen Tom Tanjeko Ajime Hannelie Korf Karen Maes Nele Heulens Conny Gysemans Chantal Mathieu Bart Vanaudenaerde Wim Janssens Ghislaine Gayan-Ramirez Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency Data in Brief |
| title | Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency |
| title_full | Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency |
| title_fullStr | Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency |
| title_full_unstemmed | Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency |
| title_short | Data on inflammatory cytokines and pathways involved in clearance of Nontypeable Haemophilus influenzae from the lungs during cigarette smoking and vitamin D deficiency |
| title_sort | data on inflammatory cytokines and pathways involved in clearance of nontypeable haemophilus influenzae from the lungs during cigarette smoking and vitamin d deficiency |
| url | http://www.sciencedirect.com/science/article/pii/S2352340918315877 |
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