Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infection
Abstract Background It has become increasingly clear that symbionts have crucial evolutionary and ecological ramifications for their host arthropods. However, little is known whether these symbiont infections influence the proteome and lysine acetylome of their host arthropods. Here we performed exp...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-07-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-018-4907-3 |
| _version_ | 1818971528069382144 |
|---|---|
| author | Hongran Li James D. Harwood Tongxian Liu Dong Chu |
| author_facet | Hongran Li James D. Harwood Tongxian Liu Dong Chu |
| author_sort | Hongran Li |
| collection | DOAJ |
| description | Abstract Background It has become increasingly clear that symbionts have crucial evolutionary and ecological ramifications for their host arthropods. However, little is known whether these symbiont infections influence the proteome and lysine acetylome of their host arthropods. Here we performed experiments to investigate the proteomes and acetylomes of Cardinium-infected (C*+) and -uninfected (C−) Bemisia tabaci Q with identical backgrounds, through the combination of affinity enrichment and high-resolution LC-MS/MS analysis. Results Of the 3353 proteins whose levels were quantitated in proteome, a total of 146 proteins dividing into 77 up-regulated and 69 down-regulated proteins were discovered to be differentially expressed as having at least a 1.2-fold change when C*+ strain was compared with C− strain. Furthermore, a total of 528 lysine acetylation sites in 283 protein groups were identified, among which 356 sites in 202 proteins were quantified. The comparison of acetylomes revealed 30 sites in 26 lysine acetylation proteins (Kac) were quantified as up-regulated targets and 35 sites in 29 Kac proteins were quantified as down-regulated targets. Functional analysis showed that these differentially expressed proteins and Kac proteins were mainly involved in diverse physiological processes related to development, immune responses and energy metabolism, such as retinol metabolism, methane metabolism and fatty acid degradation. Notably, protein interaction network analyses demonstrated widespread interactions modulated by protein acetylation. Conclusion Here we show the proteome and acetylom of B. tabaci Q in response to the symbiont Cardinium infection. This is the first study to utilize the tool of acetylome analysis for revealing physiological responses of arthropods to its symbiont infection, which will provide an important resource for exploring the arthropod-symbiont interaction. |
| first_indexed | 2024-12-20T14:53:48Z |
| format | Article |
| id | doaj.art-0fa7a741cbad49aebac79b7cce015b9d |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-20T14:53:48Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-0fa7a741cbad49aebac79b7cce015b9d2022-12-21T19:36:55ZengBMCBMC Genomics1471-21642018-07-0119111210.1186/s12864-018-4907-3Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infectionHongran Li0James D. Harwood1Tongxian Liu2Dong Chu3Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural UniversityKey Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural UniversityKey Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural UniversityKey Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural UniversityAbstract Background It has become increasingly clear that symbionts have crucial evolutionary and ecological ramifications for their host arthropods. However, little is known whether these symbiont infections influence the proteome and lysine acetylome of their host arthropods. Here we performed experiments to investigate the proteomes and acetylomes of Cardinium-infected (C*+) and -uninfected (C−) Bemisia tabaci Q with identical backgrounds, through the combination of affinity enrichment and high-resolution LC-MS/MS analysis. Results Of the 3353 proteins whose levels were quantitated in proteome, a total of 146 proteins dividing into 77 up-regulated and 69 down-regulated proteins were discovered to be differentially expressed as having at least a 1.2-fold change when C*+ strain was compared with C− strain. Furthermore, a total of 528 lysine acetylation sites in 283 protein groups were identified, among which 356 sites in 202 proteins were quantified. The comparison of acetylomes revealed 30 sites in 26 lysine acetylation proteins (Kac) were quantified as up-regulated targets and 35 sites in 29 Kac proteins were quantified as down-regulated targets. Functional analysis showed that these differentially expressed proteins and Kac proteins were mainly involved in diverse physiological processes related to development, immune responses and energy metabolism, such as retinol metabolism, methane metabolism and fatty acid degradation. Notably, protein interaction network analyses demonstrated widespread interactions modulated by protein acetylation. Conclusion Here we show the proteome and acetylom of B. tabaci Q in response to the symbiont Cardinium infection. This is the first study to utilize the tool of acetylome analysis for revealing physiological responses of arthropods to its symbiont infection, which will provide an important resource for exploring the arthropod-symbiont interaction.http://link.springer.com/article/10.1186/s12864-018-4907-3Bemisia tabaci QCardiniumPhysiological responseProteomesAcetylomes |
| spellingShingle | Hongran Li James D. Harwood Tongxian Liu Dong Chu Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infection BMC Genomics Bemisia tabaci Q Cardinium Physiological response Proteomes Acetylomes |
| title | Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infection |
| title_full | Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infection |
| title_fullStr | Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infection |
| title_full_unstemmed | Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infection |
| title_short | Novel proteome and acetylome of Bemisia tabaci Q in response to Cardinium infection |
| title_sort | novel proteome and acetylome of bemisia tabaci q in response to cardinium infection |
| topic | Bemisia tabaci Q Cardinium Physiological response Proteomes Acetylomes |
| url | http://link.springer.com/article/10.1186/s12864-018-4907-3 |
| work_keys_str_mv | AT hongranli novelproteomeandacetylomeofbemisiatabaciqinresponsetocardiniuminfection AT jamesdharwood novelproteomeandacetylomeofbemisiatabaciqinresponsetocardiniuminfection AT tongxianliu novelproteomeandacetylomeofbemisiatabaciqinresponsetocardiniuminfection AT dongchu novelproteomeandacetylomeofbemisiatabaciqinresponsetocardiniuminfection |