The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells
Abstract Background Human dental pulp stem cells (hDPSCs) may be the best choice for self-repair and regeneration of teeth and maxillofacial bone tissue due to their homogeneous tissue origin, high proliferation and differentiation rates, and no obvious ethical restrictions. Recently, several studie...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-023-02905-3 |
| _version_ | 1797849811675774976 |
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| author | Pingmeng Deng Jing Huang Qixuan Zhang Yuejia Li Jie Li |
| author_facet | Pingmeng Deng Jing Huang Qixuan Zhang Yuejia Li Jie Li |
| author_sort | Pingmeng Deng |
| collection | DOAJ |
| description | Abstract Background Human dental pulp stem cells (hDPSCs) may be the best choice for self-repair and regeneration of teeth and maxillofacial bone tissue due to their homogeneous tissue origin, high proliferation and differentiation rates, and no obvious ethical restrictions. Recently, several studies have shown that extracellular matrix (ECM) proteins can effectively regulate the proliferation and differentiation fate of mesenchymal stem cells (MSCs). However, the role of elastin microfibril interface-located protein-1 (EMILIN-1), a new ECM glycoprotein, in osteo/odontogenic differentiation of hDPSCs has not been reported. The aim of this study was to explore the effect of EMILIN-1 during osteo/odontogenic differentiation of hDPSCs. Methods hDPSCs were cultured in osteo/odontogenic induction medium. qPCR and Western blot analysis were performed to detect osteo/odonto-specific genes/proteins expression as well as the expression of EMILIN-1. After knockdown of Emilin-1 in hDPSCs with small interfering RNA and exogenous addition of recombinant human EMILIN-1 protein (rhEMILIN-1), Cell Counting Kit-8 assay, alkaline phosphatase staining, alizarin red S staining, qPCR and Western blot were performed to examine the effect of EMILIN-1 on proliferation and osteo/odontogenic differentiation of hDPSCs. Results During the osteo/odontogenic induction of hDPSCs, the expression of osteo/odonto-specific genes/proteins increased, as did EMILIN-1 protein levels. More notably, knockdown of Emilin-1 decreased hDPSCs proliferation and osteo/odontogenic differentiation, whereas exogenous addition of rhEMILIN-1 increased them. Conclusions These findings suggested that EMILIN-1 is essential for the osteo/odontogenic differentiation of hDPSCs, which may provide new insights for teeth and bone tissue regeneration. |
| first_indexed | 2024-04-09T18:50:13Z |
| format | Article |
| id | doaj.art-0fa7f800fc904119955f36ee706c2126 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-09T18:50:13Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-0fa7f800fc904119955f36ee706c21262023-04-09T11:28:45ZengBMCBMC Oral Health1472-68312023-04-0123111310.1186/s12903-023-02905-3The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cellsPingmeng Deng0Jing Huang1Qixuan Zhang2Yuejia Li3Jie Li4College of Stomatology, Chongqing Medical UniversityCollege of Stomatology, Chongqing Medical UniversityCollege of Stomatology, Chongqing Medical UniversityCollege of Stomatology, Chongqing Medical UniversityCollege of Stomatology, Chongqing Medical UniversityAbstract Background Human dental pulp stem cells (hDPSCs) may be the best choice for self-repair and regeneration of teeth and maxillofacial bone tissue due to their homogeneous tissue origin, high proliferation and differentiation rates, and no obvious ethical restrictions. Recently, several studies have shown that extracellular matrix (ECM) proteins can effectively regulate the proliferation and differentiation fate of mesenchymal stem cells (MSCs). However, the role of elastin microfibril interface-located protein-1 (EMILIN-1), a new ECM glycoprotein, in osteo/odontogenic differentiation of hDPSCs has not been reported. The aim of this study was to explore the effect of EMILIN-1 during osteo/odontogenic differentiation of hDPSCs. Methods hDPSCs were cultured in osteo/odontogenic induction medium. qPCR and Western blot analysis were performed to detect osteo/odonto-specific genes/proteins expression as well as the expression of EMILIN-1. After knockdown of Emilin-1 in hDPSCs with small interfering RNA and exogenous addition of recombinant human EMILIN-1 protein (rhEMILIN-1), Cell Counting Kit-8 assay, alkaline phosphatase staining, alizarin red S staining, qPCR and Western blot were performed to examine the effect of EMILIN-1 on proliferation and osteo/odontogenic differentiation of hDPSCs. Results During the osteo/odontogenic induction of hDPSCs, the expression of osteo/odonto-specific genes/proteins increased, as did EMILIN-1 protein levels. More notably, knockdown of Emilin-1 decreased hDPSCs proliferation and osteo/odontogenic differentiation, whereas exogenous addition of rhEMILIN-1 increased them. Conclusions These findings suggested that EMILIN-1 is essential for the osteo/odontogenic differentiation of hDPSCs, which may provide new insights for teeth and bone tissue regeneration.https://doi.org/10.1186/s12903-023-02905-3Elastin microfibril interface-located protein-1Human dental pulp stem cellsOsteo/Odontogenic differentiationTooth regenerationBone regeneration |
| spellingShingle | Pingmeng Deng Jing Huang Qixuan Zhang Yuejia Li Jie Li The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells BMC Oral Health Elastin microfibril interface-located protein-1 Human dental pulp stem cells Osteo/Odontogenic differentiation Tooth regeneration Bone regeneration |
| title | The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells |
| title_full | The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells |
| title_fullStr | The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells |
| title_full_unstemmed | The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells |
| title_short | The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells |
| title_sort | role of emilin 1 in the osteo odontogenic differentiation of dental pulp stem cells |
| topic | Elastin microfibril interface-located protein-1 Human dental pulp stem cells Osteo/Odontogenic differentiation Tooth regeneration Bone regeneration |
| url | https://doi.org/10.1186/s12903-023-02905-3 |
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