Antimicrobial activity of Pantanal macrophytes against multidrug resistant bacteria shows potential for improving nature-based solutions
The presence of macrophytes in constructed wetlands (CW) applied within the concept of nature-based solution (NBS) is crucial. Besides their role in the phytoremediation of pollutants, the cultivated plants, if properly selected, can also produce a range of bioactive compounds, including those with...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Plant Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X23001719 |
| _version_ | 1797448712611430400 |
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| author | Karen Midori Takahashi Juliano Akio Nakasato Genilson Silva de Jesus Ana Camila Michelletti Arnildo Pott Nídia Cristiane Yoshida Paula Loureiro Paulo |
| author_facet | Karen Midori Takahashi Juliano Akio Nakasato Genilson Silva de Jesus Ana Camila Michelletti Arnildo Pott Nídia Cristiane Yoshida Paula Loureiro Paulo |
| author_sort | Karen Midori Takahashi |
| collection | DOAJ |
| description | The presence of macrophytes in constructed wetlands (CW) applied within the concept of nature-based solution (NBS) is crucial. Besides their role in the phytoremediation of pollutants, the cultivated plants, if properly selected, can also produce a range of bioactive compounds, including those with potential inhibitory effects against pathogenic bacteria. Bioactive compounds, such as flavonoids and ellagitannins, known for their remarkable antimicrobial properties, offer a sustainable solution to the global challenge of antibiotic resistance. In this study, we investigated macrophyte species adapted to the water variations in the Pantanal region (flood and dry seasons), focusing on those with both antimicrobial and ornamental potential, that could improve the ability of a CW system to treat greywater. Seven different macrophyte species were collected, cultivated, and evaluated for their antimicrobial activity (AA) against five standard bacterial strains and five multidrug-resistant clinical bacterial strains. Extracts from the leaf, stem, and root of Polygonum acuminatum showed the best AA against clinical multidrug-resistant strains of Staphylococcus aureus, Enterococcus faecium, and Escherichia coli, with minimum inhibitory concentration (MIC) values ranging from 78.13 to 312.5 µg.mL−1. Furthermore, the leaves of Ludwigia lagunae and P. acuminatum also showed considerable AA against standard strains of Enterococcus faecalis (MIC = 19.5 and 39.06 µg.mL−1, respectively) and Pseudomonas aeruginosa (MIC = 156.25 µg.mL−1 for both). High-Performance Liquid Chromatography with Ultraviolet and Mass Spectrometry analysis of these extracts identified important bioactive compounds, including flavonoids (quercetin 3-rhamnoside, hydropiperoside, quercetin 3-O-rutinoside, quercetin, myricetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside, formononetin, and calycosin) and ellagitannins (3,3′4-tri-O-methylelagic acid, galloys-HHDP-glucose, and ellagic acid), along with other bioactive compounds. These results indicate that P. acuminatum and L. lagunae hold potential for application in CW owing to their antimicrobial property, local adaptability, and ornamental appeal. |
| first_indexed | 2024-03-09T14:14:24Z |
| format | Article |
| id | doaj.art-0faac788a296428f8c750b38c6213de2 |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-03-09T14:14:24Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-0faac788a296428f8c750b38c6213de22023-11-29T04:25:05ZengElsevierPlant Stress2667-064X2024-03-0111100304Antimicrobial activity of Pantanal macrophytes against multidrug resistant bacteria shows potential for improving nature-based solutionsKaren Midori Takahashi0Juliano Akio Nakasato1Genilson Silva de Jesus2Ana Camila Michelletti3Arnildo Pott4Nídia Cristiane Yoshida5Paula Loureiro Paulo6Faculty of Engineering, Architecture and Urbanism, and Geography, Federal University of Mato Grosso do Sul, Av. Costa e Silva, s/n, Campo Grande, MS, 79070900, Brazil; Corresponding author.Faculty of Engineering, Architecture and Urbanism, and Geography, Federal University of Mato Grosso do Sul, Av. Costa e Silva, s/n, Campo Grande, MS, 79070900, BrazilChemistry Institute, Federal University of Mato Grosso do Sul, Av. Senador Filinto Müler, 1555, Campo Grande, MS, 79074460, BrazilChemistry Institute, Federal University of Mato Grosso do Sul, Av. Senador Filinto Müler, 1555, Campo Grande, MS, 79074460, BrazilFaculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Av. Costa e Silva, s/n, Campo Grande, MS, 79070900, BrazilChemistry Institute, Federal University of Mato Grosso do Sul, Av. Senador Filinto Müler, 1555, Campo Grande, MS, 79074460, BrazilFaculty of Engineering, Architecture and Urbanism, and Geography, Federal University of Mato Grosso do Sul, Av. Costa e Silva, s/n, Campo Grande, MS, 79070900, BrazilThe presence of macrophytes in constructed wetlands (CW) applied within the concept of nature-based solution (NBS) is crucial. Besides their role in the phytoremediation of pollutants, the cultivated plants, if properly selected, can also produce a range of bioactive compounds, including those with potential inhibitory effects against pathogenic bacteria. Bioactive compounds, such as flavonoids and ellagitannins, known for their remarkable antimicrobial properties, offer a sustainable solution to the global challenge of antibiotic resistance. In this study, we investigated macrophyte species adapted to the water variations in the Pantanal region (flood and dry seasons), focusing on those with both antimicrobial and ornamental potential, that could improve the ability of a CW system to treat greywater. Seven different macrophyte species were collected, cultivated, and evaluated for their antimicrobial activity (AA) against five standard bacterial strains and five multidrug-resistant clinical bacterial strains. Extracts from the leaf, stem, and root of Polygonum acuminatum showed the best AA against clinical multidrug-resistant strains of Staphylococcus aureus, Enterococcus faecium, and Escherichia coli, with minimum inhibitory concentration (MIC) values ranging from 78.13 to 312.5 µg.mL−1. Furthermore, the leaves of Ludwigia lagunae and P. acuminatum also showed considerable AA against standard strains of Enterococcus faecalis (MIC = 19.5 and 39.06 µg.mL−1, respectively) and Pseudomonas aeruginosa (MIC = 156.25 µg.mL−1 for both). High-Performance Liquid Chromatography with Ultraviolet and Mass Spectrometry analysis of these extracts identified important bioactive compounds, including flavonoids (quercetin 3-rhamnoside, hydropiperoside, quercetin 3-O-rutinoside, quercetin, myricetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside, formononetin, and calycosin) and ellagitannins (3,3′4-tri-O-methylelagic acid, galloys-HHDP-glucose, and ellagic acid), along with other bioactive compounds. These results indicate that P. acuminatum and L. lagunae hold potential for application in CW owing to their antimicrobial property, local adaptability, and ornamental appeal.http://www.sciencedirect.com/science/article/pii/S2667064X23001719resistant bacteriaplant extractsornamental plantsantibacterial activitybioactive compounds |
| spellingShingle | Karen Midori Takahashi Juliano Akio Nakasato Genilson Silva de Jesus Ana Camila Michelletti Arnildo Pott Nídia Cristiane Yoshida Paula Loureiro Paulo Antimicrobial activity of Pantanal macrophytes against multidrug resistant bacteria shows potential for improving nature-based solutions Plant Stress resistant bacteria plant extracts ornamental plants antibacterial activity bioactive compounds |
| title | Antimicrobial activity of Pantanal macrophytes against multidrug resistant bacteria shows potential for improving nature-based solutions |
| title_full | Antimicrobial activity of Pantanal macrophytes against multidrug resistant bacteria shows potential for improving nature-based solutions |
| title_fullStr | Antimicrobial activity of Pantanal macrophytes against multidrug resistant bacteria shows potential for improving nature-based solutions |
| title_full_unstemmed | Antimicrobial activity of Pantanal macrophytes against multidrug resistant bacteria shows potential for improving nature-based solutions |
| title_short | Antimicrobial activity of Pantanal macrophytes against multidrug resistant bacteria shows potential for improving nature-based solutions |
| title_sort | antimicrobial activity of pantanal macrophytes against multidrug resistant bacteria shows potential for improving nature based solutions |
| topic | resistant bacteria plant extracts ornamental plants antibacterial activity bioactive compounds |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X23001719 |
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