Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals
How cells sense their mechanical environment and transduce forces into biochemical signals is a crucial yet unresolved question in mechanobiology. Platelets use receptor glycoprotein Ib (GPIb), specifically its α subunit (GPIbα), to signal as they tether and translocate on von Willebrand factor (VWF...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-07-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/15447 |
| _version_ | 1828375587855532032 |
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| author | Lining Ju Yunfeng Chen Lingzhou Xue Xiaoping Du Cheng Zhu |
| author_facet | Lining Ju Yunfeng Chen Lingzhou Xue Xiaoping Du Cheng Zhu |
| author_sort | Lining Ju |
| collection | DOAJ |
| description | How cells sense their mechanical environment and transduce forces into biochemical signals is a crucial yet unresolved question in mechanobiology. Platelets use receptor glycoprotein Ib (GPIb), specifically its α subunit (GPIbα), to signal as they tether and translocate on von Willebrand factor (VWF) of injured arterial surfaces against blood flow. Force elicits catch bonds to slow VWF–GPIbα dissociation and unfolds the GPIbα leucine-rich repeat domain (LRRD) and juxtamembrane mechanosensitive domain (MSD). How these mechanical processes trigger biochemical signals remains unknown. Here we analyze these extracellular events and the resulting intracellular Ca2+ on a single platelet in real time, revealing that LRRD unfolding intensifies Ca2+ signal whereas MSD unfolding affects the type of Ca2+ signal. Therefore, LRRD and MSD are analog and digital force transducers, respectively. The >30 nm macroglycopeptide separating the two domains transmits force on the VWF–GPIbα bond (whose lifetime is prolonged by LRRD unfolding) to the MSD to enhance its unfolding, resulting in unfolding cooperativity at an optimal force. These elements may provide design principles for a generic mechanosensory protein machine. |
| first_indexed | 2024-04-14T07:48:34Z |
| format | Article |
| id | doaj.art-0facf10e6d57497caa211e9217b70e9d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:48:34Z |
| publishDate | 2016-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-0facf10e6d57497caa211e9217b70e9d2022-12-22T02:05:15ZengeLife Sciences Publications LtdeLife2050-084X2016-07-01510.7554/eLife.15447Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signalsLining Ju0Yunfeng Chen1Lingzhou Xue2Xiaoping Du3Cheng Zhu4https://orcid.org/0000-0002-1718-565XCoulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, United States; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, United States; Heart Research Institute, Camperdown, Australia; Charles Perkins Centre, The University of Sydney, Camperdown, AustraliaPetit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, United States; Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, United StatesDepartment of Statistics, The Pennsylvania State University, University Park, United StatesDepartment of Pharmacology, College of Medicine, University of Illinois at Chicago, Chicago, United StatesCoulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, United States; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, United States; Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, United StatesHow cells sense their mechanical environment and transduce forces into biochemical signals is a crucial yet unresolved question in mechanobiology. Platelets use receptor glycoprotein Ib (GPIb), specifically its α subunit (GPIbα), to signal as they tether and translocate on von Willebrand factor (VWF) of injured arterial surfaces against blood flow. Force elicits catch bonds to slow VWF–GPIbα dissociation and unfolds the GPIbα leucine-rich repeat domain (LRRD) and juxtamembrane mechanosensitive domain (MSD). How these mechanical processes trigger biochemical signals remains unknown. Here we analyze these extracellular events and the resulting intracellular Ca2+ on a single platelet in real time, revealing that LRRD unfolding intensifies Ca2+ signal whereas MSD unfolding affects the type of Ca2+ signal. Therefore, LRRD and MSD are analog and digital force transducers, respectively. The >30 nm macroglycopeptide separating the two domains transmits force on the VWF–GPIbα bond (whose lifetime is prolonged by LRRD unfolding) to the MSD to enhance its unfolding, resulting in unfolding cooperativity at an optimal force. These elements may provide design principles for a generic mechanosensory protein machine.https://elifesciences.org/articles/15447mechanosensingsingle moleculeleucine rich repeatGPIbplateletcalcium signaling |
| spellingShingle | Lining Ju Yunfeng Chen Lingzhou Xue Xiaoping Du Cheng Zhu Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals eLife mechanosensing single molecule leucine rich repeat GPIb platelet calcium signaling |
| title | Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals |
| title_full | Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals |
| title_fullStr | Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals |
| title_full_unstemmed | Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals |
| title_short | Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals |
| title_sort | cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals |
| topic | mechanosensing single molecule leucine rich repeat GPIb platelet calcium signaling |
| url | https://elifesciences.org/articles/15447 |
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