The role of local backrub motions in evolved and designed mutations.
Amino acid substitutions in protein structures often require subtle backbone adjustments that are difficult to model in atomic detail. An improved ability to predict realistic backbone changes in response to engineered mutations would be of great utility for the blossoming field of rational protein...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC3410847?pdf=render |
| _version_ | 1818577915090042880 |
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| author | Daniel A Keedy Ivelin Georgiev Edward B Triplett Bruce R Donald David C Richardson Jane S Richardson |
| author_facet | Daniel A Keedy Ivelin Georgiev Edward B Triplett Bruce R Donald David C Richardson Jane S Richardson |
| author_sort | Daniel A Keedy |
| collection | DOAJ |
| description | Amino acid substitutions in protein structures often require subtle backbone adjustments that are difficult to model in atomic detail. An improved ability to predict realistic backbone changes in response to engineered mutations would be of great utility for the blossoming field of rational protein design. One model that has recently grown in acceptance is the backrub motion, a low-energy dipeptide rotation with single-peptide counter-rotations, that is coupled to dynamic two-state sidechain rotamer jumps, as evidenced by alternate conformations in very high-resolution crystal structures. It has been speculated that backrubs may facilitate sequence changes equally well as rotamer changes. However, backrub-induced shifts and experimental uncertainty are of similar magnitude for backbone atoms in even high-resolution structures, so comparison of wildtype-vs.-mutant crystal structure pairs is not sufficient to directly link backrubs to mutations. In this study, we use two alternative approaches that bypass this limitation. First, we use a quality-filtered structure database to aggregate many examples for precisely defined motifs with single amino acid differences, and find that the effectively amplified backbone differences closely resemble backrubs. Second, we directly apply a provably-accurate, backrub-enabled protein design algorithm to idealized versions of these motifs, and discover that the lowest-energy computed models match the average-coordinate experimental structures. These results support the hypothesis that backrubs participate in natural protein evolution and validate their continued use for design of synthetic proteins. |
| first_indexed | 2024-12-16T06:37:29Z |
| format | Article |
| id | doaj.art-0fafa1920b8342ebad92d96d402abcb8 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-16T06:37:29Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-0fafa1920b8342ebad92d96d402abcb82022-12-21T22:40:46ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582012-01-0188e100262910.1371/journal.pcbi.1002629The role of local backrub motions in evolved and designed mutations.Daniel A KeedyIvelin GeorgievEdward B TriplettBruce R DonaldDavid C RichardsonJane S RichardsonAmino acid substitutions in protein structures often require subtle backbone adjustments that are difficult to model in atomic detail. An improved ability to predict realistic backbone changes in response to engineered mutations would be of great utility for the blossoming field of rational protein design. One model that has recently grown in acceptance is the backrub motion, a low-energy dipeptide rotation with single-peptide counter-rotations, that is coupled to dynamic two-state sidechain rotamer jumps, as evidenced by alternate conformations in very high-resolution crystal structures. It has been speculated that backrubs may facilitate sequence changes equally well as rotamer changes. However, backrub-induced shifts and experimental uncertainty are of similar magnitude for backbone atoms in even high-resolution structures, so comparison of wildtype-vs.-mutant crystal structure pairs is not sufficient to directly link backrubs to mutations. In this study, we use two alternative approaches that bypass this limitation. First, we use a quality-filtered structure database to aggregate many examples for precisely defined motifs with single amino acid differences, and find that the effectively amplified backbone differences closely resemble backrubs. Second, we directly apply a provably-accurate, backrub-enabled protein design algorithm to idealized versions of these motifs, and discover that the lowest-energy computed models match the average-coordinate experimental structures. These results support the hypothesis that backrubs participate in natural protein evolution and validate their continued use for design of synthetic proteins.http://europepmc.org/articles/PMC3410847?pdf=render |
| spellingShingle | Daniel A Keedy Ivelin Georgiev Edward B Triplett Bruce R Donald David C Richardson Jane S Richardson The role of local backrub motions in evolved and designed mutations. PLoS Computational Biology |
| title | The role of local backrub motions in evolved and designed mutations. |
| title_full | The role of local backrub motions in evolved and designed mutations. |
| title_fullStr | The role of local backrub motions in evolved and designed mutations. |
| title_full_unstemmed | The role of local backrub motions in evolved and designed mutations. |
| title_short | The role of local backrub motions in evolved and designed mutations. |
| title_sort | role of local backrub motions in evolved and designed mutations |
| url | http://europepmc.org/articles/PMC3410847?pdf=render |
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