| Summary: | Plant biostimulants are being recognized as innovative tools to improve sustainable agricultural practices to mitigate the drastic effects of climate change, which is leading to a severe reduction in agricultural yields. In this work, a new biostimulant (EnNuVi<sup>®</sup> ALPAN<sup>®</sup>) was evaluated for its effectiveness on tomato (<i>Solanum lycopersicum</i> Mill. cv. Rio Grande) plants subjected to water deficit conditions. The molecular effects were elucidated through transcriptomic RNA-seq and gene expression qPCR analysis and the physiological responses were evaluated through qualitative analysis of pigments and proline content, membrane stability, and lipid peroxidation. ALPAN<sup>®</sup> was shown to adjust the transcriptional response by upregulating genes involved in source to sink carbohydrate metabolism and translocation, stomatal closure, and cell homeostasis. ALPAN<sup>®</sup> was shown to mitigate the deteriorating effects of water deficit on the physiological status of the plants by stabilizing the levels of the photosynthetic pigments, regulating the accumulation of osmo-protectants, and preserving the cell wall lipid bilayer from oxidation. In conclusion, transcriptomic and physiological analysis provided insightful information on the biostimulant effects, indicating a positive role of ALPAN<sup>®</sup> foliar application in alleviating the negative costs of water deficit.
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