Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome
Sphingolipids are ubiquitous in eukaryotes and certain prokaryotes, where they serve as vital components of biological membranes and bioactive molecules. Chloroplasts have complex membrane structures that play crucial roles in photosynthesis, but their specific sphingolipidome remains unreported. In...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/13/2/299 |
| _version_ | 1797339387885780992 |
|---|---|
| author | Chang Yang Ling-Yan Wang Yong-Kang Li Jia-Ting Lin Ding-Kang Chen Nan Yao |
| author_facet | Chang Yang Ling-Yan Wang Yong-Kang Li Jia-Ting Lin Ding-Kang Chen Nan Yao |
| author_sort | Chang Yang |
| collection | DOAJ |
| description | Sphingolipids are ubiquitous in eukaryotes and certain prokaryotes, where they serve as vital components of biological membranes and bioactive molecules. Chloroplasts have complex membrane structures that play crucial roles in photosynthesis, but their specific sphingolipidome remains unreported. In this study, we used liquid chromatography–mass spectrometry (LC-MS/MS) to analyze the sphingolipidome of purified <i>Arabidopsis thaliana</i> chloroplasts. We detected 92 chloroplast sphingolipids. The chloroplast sphingolipidome differed from total leaf (TL) samples, with a higher content of free long-chain bases and hydroxyceramides and a greater proportion of complex sphingolipids with 16C fatty acid (FA) forms. Notably, chloroplast glucosylceramides were predominantly the d18:1 h16:0 and t18:1 h16:0 forms rather than the 24C FA form found in TL and other cellular structures. Comparing the sphingolipidomes of different cellular structures underscores the inhomogeneity of the intracellular distribution of sphingolipids. This provides a robust reference for further elucidating the function of sphingolipids in plant cells. |
| first_indexed | 2024-03-08T09:47:31Z |
| format | Article |
| id | doaj.art-0fb23474620b4484b37e428909eff686 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T09:47:31Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-0fb23474620b4484b37e428909eff6862024-01-29T14:12:06ZengMDPI AGPlants2223-77472024-01-0113229910.3390/plants13020299Arabidopsis Leaf Chloroplasts Have a Specific SphingolipidomeChang Yang0Ling-Yan Wang1Yong-Kang Li2Jia-Ting Lin3Ding-Kang Chen4Nan Yao5State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaSphingolipids are ubiquitous in eukaryotes and certain prokaryotes, where they serve as vital components of biological membranes and bioactive molecules. Chloroplasts have complex membrane structures that play crucial roles in photosynthesis, but their specific sphingolipidome remains unreported. In this study, we used liquid chromatography–mass spectrometry (LC-MS/MS) to analyze the sphingolipidome of purified <i>Arabidopsis thaliana</i> chloroplasts. We detected 92 chloroplast sphingolipids. The chloroplast sphingolipidome differed from total leaf (TL) samples, with a higher content of free long-chain bases and hydroxyceramides and a greater proportion of complex sphingolipids with 16C fatty acid (FA) forms. Notably, chloroplast glucosylceramides were predominantly the d18:1 h16:0 and t18:1 h16:0 forms rather than the 24C FA form found in TL and other cellular structures. Comparing the sphingolipidomes of different cellular structures underscores the inhomogeneity of the intracellular distribution of sphingolipids. This provides a robust reference for further elucidating the function of sphingolipids in plant cells.https://www.mdpi.com/2223-7747/13/2/299<i>Arabidopsis thaliana</i>chloroplastsphingolipidomeLC-MS/MS |
| spellingShingle | Chang Yang Ling-Yan Wang Yong-Kang Li Jia-Ting Lin Ding-Kang Chen Nan Yao Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome Plants <i>Arabidopsis thaliana</i> chloroplast sphingolipidome LC-MS/MS |
| title | Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome |
| title_full | Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome |
| title_fullStr | Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome |
| title_full_unstemmed | Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome |
| title_short | Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome |
| title_sort | arabidopsis leaf chloroplasts have a specific sphingolipidome |
| topic | <i>Arabidopsis thaliana</i> chloroplast sphingolipidome LC-MS/MS |
| url | https://www.mdpi.com/2223-7747/13/2/299 |
| work_keys_str_mv | AT changyang arabidopsisleafchloroplastshaveaspecificsphingolipidome AT lingyanwang arabidopsisleafchloroplastshaveaspecificsphingolipidome AT yongkangli arabidopsisleafchloroplastshaveaspecificsphingolipidome AT jiatinglin arabidopsisleafchloroplastshaveaspecificsphingolipidome AT dingkangchen arabidopsisleafchloroplastshaveaspecificsphingolipidome AT nanyao arabidopsisleafchloroplastshaveaspecificsphingolipidome |