Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from <i>Thalictrum foetidum</i>, <i>Berberis</i> sp. and <i>Chelidonium majus</i> Extracts
<i>Background:</i> Plants are an important origin of natural substances that the raw material for various pharmaceutical and therapeutic applications due to the presence of phytochemicals, such as alkaloids. Alkaloids, which are found in different plant species, possess numerous biologic...
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MDPI AG
2019-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/19/3417 |
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| author | Anna Petruczynik Tomasz Tuzimski Tomasz Plech Justyna Misiurek Karolina Szalast Grażyna Szymczak |
| author_facet | Anna Petruczynik Tomasz Tuzimski Tomasz Plech Justyna Misiurek Karolina Szalast Grażyna Szymczak |
| author_sort | Anna Petruczynik |
| collection | DOAJ |
| description | <i>Background:</i> Plants are an important origin of natural substances that the raw material for various pharmaceutical and therapeutic applications due to the presence of phytochemicals, such as alkaloids. Alkaloids, which are found in different plant species, possess numerous biological activities. Some alkaloids have strong cytotoxic effects on various cancer cells. The search for new drugs to treat various cancers is one of the most important challenges of modern scientific research. <i>Objective:</i> This study aimed to investigate of cytotoxic activity of extracts that were obtained from <i>Chelidonium Majus</i>; <i>Berberis</i> sp.; <i>Thalictrum foetidum</i> containing various alkaloids on selected cancer cell lines. The aim was also the quantification of selected alkaloids in the investigated extracts by HPLC. <i>Methods:</i> The analysis of alkaloids contents were performed while using HPLC in reversed phase (RP) mode using Polar RP column and mobile phase containing acetonitrile, water, and ionic liquid. The cytotoxic effect of the tested plant extracts and respective alkaloids’ standards were examined while using human pharyngeal squamous carcinoma cells (FaDu), human tongue squamous carcinoma cells (SCC-25), human breast adenocarcinoma cell line (MCF-7), and human triple-negative breast adenocarcinoma cell line (MDA-MB-231). <i>Conclusion:</i> All of the investigated plant extracts possess cytotoxic activity against cancer cell lines: FaDu, SCC-25, MCF-7, and MDA-MB-231. The highest cytotoxic activity against FaDu and MDA-MB-231 cells was observed for <i>Chelidonium majus</i> root extract, while the highest cytotoxic activity against SCC-25 and MCF-7 cells was estimated for the <i>Thalictrum foetidum</i> root extract. There obtained significant differences in the cytotoxic activity of extracts that were obtained from the roots and herbs of <i>Chelidonium majus</i> and <i>Thalictrum foetidum.</i> Based on these results, investigated plant extracts can be recommended for further investigations of anticancer activity. |
| first_indexed | 2024-12-21T19:53:20Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T19:53:20Z |
| publishDate | 2019-09-01 |
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| spelling | doaj.art-0fb3c2e534c8425eab6bacfb433040992022-12-21T18:52:09ZengMDPI AGMolecules1420-30492019-09-012419341710.3390/molecules24193417molecules24193417Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from <i>Thalictrum foetidum</i>, <i>Berberis</i> sp. and <i>Chelidonium majus</i> ExtractsAnna Petruczynik0Tomasz Tuzimski1Tomasz Plech2Justyna Misiurek3Karolina Szalast4Grażyna Szymczak5Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Pharmacology, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Inorganic Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Pharmacology, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandBotanical Garden of Maria Curie-Skłodowska University in Lublin, 20-819 Lublin, Poland<i>Background:</i> Plants are an important origin of natural substances that the raw material for various pharmaceutical and therapeutic applications due to the presence of phytochemicals, such as alkaloids. Alkaloids, which are found in different plant species, possess numerous biological activities. Some alkaloids have strong cytotoxic effects on various cancer cells. The search for new drugs to treat various cancers is one of the most important challenges of modern scientific research. <i>Objective:</i> This study aimed to investigate of cytotoxic activity of extracts that were obtained from <i>Chelidonium Majus</i>; <i>Berberis</i> sp.; <i>Thalictrum foetidum</i> containing various alkaloids on selected cancer cell lines. The aim was also the quantification of selected alkaloids in the investigated extracts by HPLC. <i>Methods:</i> The analysis of alkaloids contents were performed while using HPLC in reversed phase (RP) mode using Polar RP column and mobile phase containing acetonitrile, water, and ionic liquid. The cytotoxic effect of the tested plant extracts and respective alkaloids’ standards were examined while using human pharyngeal squamous carcinoma cells (FaDu), human tongue squamous carcinoma cells (SCC-25), human breast adenocarcinoma cell line (MCF-7), and human triple-negative breast adenocarcinoma cell line (MDA-MB-231). <i>Conclusion:</i> All of the investigated plant extracts possess cytotoxic activity against cancer cell lines: FaDu, SCC-25, MCF-7, and MDA-MB-231. The highest cytotoxic activity against FaDu and MDA-MB-231 cells was observed for <i>Chelidonium majus</i> root extract, while the highest cytotoxic activity against SCC-25 and MCF-7 cells was estimated for the <i>Thalictrum foetidum</i> root extract. There obtained significant differences in the cytotoxic activity of extracts that were obtained from the roots and herbs of <i>Chelidonium majus</i> and <i>Thalictrum foetidum.</i> Based on these results, investigated plant extracts can be recommended for further investigations of anticancer activity.https://www.mdpi.com/1420-3049/24/19/3417isoquinoline alkaloidsHPLC-DAD<i>Chelidonium majus</i><i>Berberis</i> sp.<i>Thalictrum foetidum</i>cytotoxic activityetoposide |
| spellingShingle | Anna Petruczynik Tomasz Tuzimski Tomasz Plech Justyna Misiurek Karolina Szalast Grażyna Szymczak Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from <i>Thalictrum foetidum</i>, <i>Berberis</i> sp. and <i>Chelidonium majus</i> Extracts Molecules isoquinoline alkaloids HPLC-DAD <i>Chelidonium majus</i> <i>Berberis</i> sp. <i>Thalictrum foetidum</i> cytotoxic activity etoposide |
| title | Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from <i>Thalictrum foetidum</i>, <i>Berberis</i> sp. and <i>Chelidonium majus</i> Extracts |
| title_full | Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from <i>Thalictrum foetidum</i>, <i>Berberis</i> sp. and <i>Chelidonium majus</i> Extracts |
| title_fullStr | Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from <i>Thalictrum foetidum</i>, <i>Berberis</i> sp. and <i>Chelidonium majus</i> Extracts |
| title_full_unstemmed | Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from <i>Thalictrum foetidum</i>, <i>Berberis</i> sp. and <i>Chelidonium majus</i> Extracts |
| title_short | Comparison of Anticancer Activity and HPLC-DAD Determination of Selected Isoquinoline Alkaloids from <i>Thalictrum foetidum</i>, <i>Berberis</i> sp. and <i>Chelidonium majus</i> Extracts |
| title_sort | comparison of anticancer activity and hplc dad determination of selected isoquinoline alkaloids from i thalictrum foetidum i i berberis i sp and i chelidonium majus i extracts |
| topic | isoquinoline alkaloids HPLC-DAD <i>Chelidonium majus</i> <i>Berberis</i> sp. <i>Thalictrum foetidum</i> cytotoxic activity etoposide |
| url | https://www.mdpi.com/1420-3049/24/19/3417 |
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